Maidacinda, Savicalista (2017) Pengaruh Pemberian Serbuk Daun Kemangi (Ocimum Canum Sims.) Terhadap Ekspresi Nrf2 Pada Ovarium Tikus Putih (Rattus Norvergicus) Premenopause. Sarjana thesis, Universitas Brawijaya.
Abstract
Daun kemangi (Ocimum canum Sims.) merupakan salah satu sumber antioksidan eksogen yang alami karena mengandung senyawa flavonoid. Mekanisme kerja dari flavonoid dapat menangkal akumulasi ROS pada tikus premenopause. Penelitian ini bertujuan untuk mengetahui pengaruh serbuk daun kemangi (Ocimum canum) terhadap ekspresi Nrf2 pada ovarium tikus putih (Rattus novergicus) premenopause. Penelitian ini menggunakan tikus betina umur 11 bulan dengan berat badan rata-rata 180-220 g. Tikus (Rattus norvegicus) dibagi menjadi empat kelompok yaitu kontrol (K), dosis 1 (0,25 g/kg BB tikus), dosis 2 (0,5 g/kg BB tikus), dan dosis 3 (1 g/kg BB). Tikus diberi perlakuan serbuk daun kemangi setiap hari dengan cara oral selama 15 hari yang dimulai dari fase estrus yang sama. Ekspresi Nrf2 dideteksi dengan menggunakan metode imunohistokimia (IHK). Hasil ekspresi Nrf2 diuji dengan ANOVA, apabila data terdistribusi normal dan varian data sama maka analisis dilanjutkan dengan uji Tukey. Hasil penelitian pemberian serbuk daun kemangi dapat meningkatkan ekspresi Nrf2 antara kelompok kontrol dengan kelompok perlakuan.
English Abstract
Leaves of basil (Ocimum canum Sims.) is one of the sources of exogenous antioxidants because it contains compounds of flavonoids. Mechanism of flavonoids as antioxidants can warding off the accumulation of ROS in mice premenopausal. This research aims to know the influence of the powder leaves Basil (Ocimum canum) against Nrf2 expression in ovaries of white rats (Rattus novergicus) premenopausal. This research uses the female mice at the age of 11 months with the average body weight of 180-220 grams. Rat (Rattus norvegicus) is divided into four groups, controls (K), dose 1 (0.25 g/kg rat), dose 2 (0.5 g/kg rat), and dose 3 (1 g/kg). The mice were given oral treatment for 15 days starting from the same estrus phase. Nrf2 expression can be seen from the results of staining with immunohistochemical method (IHC). The result of expression Nrf2 then tested by ANOVA. After that, it will be tested by Tukey test. The result is powder basil leaves can affect expression of Nrf2 significantly between treatment group with a control group.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FMIPA/2017/317/051709657 |
| Uncontrolled Keywords: | Antioksidan, daun kemangi, flavonoid, Nrf2, radikal bebas |
| Subjects: | 500 Natural sciences and mathematics > 570 Biology > 570.7 Education, research, related topics |
| Divisions: | Fakultas Matematika dan Ilmu Pengetahuan Alam > Biologi |
| Depositing User: | Nur Cholis |
| Date Deposited: | 20 Oct 2017 02:22 |
| Last Modified: | 23 Nov 2021 07:27 |
| URI: | http://repository.ub.ac.id/id/eprint/4073 |
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