Sabillah, Ilman Rois (2017) Pengaruh Latihan Fisik (Treadmill) Dengan Peningkatan Kecepatan Secara Sekuensial Dan Durasi Terhadap Kadar Malondialdehyde (MDA) Dan Gambaran Histopatologi Testis Pada Tikus (Rattus norvegicus) Model Diabetes Mellitus Tipe 2. Sarjana thesis, Universitas Brawijaya.
Abstract
Diabetes mellitus tipe 2 (DMT2) adalah penyakit gangguan metabolik yang disebabkan oleh retensi insulin dan penurunan fungsi sel β pankreas. Stress oksidatif menyebabkan peroksidasi lipid yang berakibat terjadi kenaikan MDA dan kerusakan pada membrane mitokondria serta apoptosis pada testis. Latihan fisik (treadmill) merupakan salah satu upaya untuk mengurangi efek DMT2 dan meningkatkan homeostasis glukosa dan meningkatkan sensitifitas insulin. Penelitian ini bertujuan untuk mengetahui pengaruh latihan fisik (treadmill) terhadap kadar malondialdehid (MDA) dan gambaran histopatologi testis pada tikus (Rattus norvegicus) model DMT2. Penelitian menggunakan tiga kelompok tikus yaitu normal tanpa perlakuan, DMT2 dengan induksi pakan tinggi lemak selama 64 hari sebanyak 40 g/ekor/hari dan streptozotocin (STZ) dengan dosis 30 mg/kg BB 2 minggu dengan 1 minggu sekali, dan DMT2 dengan induksi pakan tinggi lemak sebanyak 40 g/ekor/hari dan streptozotocin (STZ) dengan dosis 30 mg/kg BB 2 minggu dengan 1 minggu sekali ditambah latihan fisik.. Latihan fisik dengan kecepatan dan durasi yang meningkat setiap dua minggu yaitu 10 m/menit selama 10 menit, 14 m/menit selama 22 menit, 18 m/menit selama 34 menit, 22 m/menit selama 46 menit, dan 27 m/menit selama 60 menit. Kadar MDA diuji secara Spektofotometri. Data MDA di uji secara kuantitatif menggunakan uji one way ANOVA α=5% dilanjutkan dengan BNT, sedangkan histopatologi testis diuji secara kualitatif deskriptif. Gambaran histopatologi testis diwarnai dengan pewarnaan Hematoxylin Eosin (HE). Hasil penelitian menunjukkan treadmill secara signifikan (p<0,05) menurunkan kadar MDA dan memperbaiki sel sertoli dan sel leydig terlihat dari tubulus semineferus yang semakin padat dan tidak terlihat adanya ruang kosong di tubulus semineferus, sehingga dapat disimpulkan bahwa latihan fisik treadmill dapat menurunkan kadar MDA dan memperbaiki histopatologis testis pada tikus model DMT2.
English Abstract
Diabetes mellitus type 2 (DMT2) is a metabolic disorder disease caused by insulin retention and decreased function of pancreatic β cells. Oxidative stress causes lipid peroxidation resulting in increased MDA and damage to the mitochondrial membrane as well as apoptosis in the testes. Physical exercise (treadmill) is one effort to reduce the effects of DMT2 and increase glucose homeostasis as well as increase insulin sensitivity. This study aim to determine the effect of physical exercise (treadmill) on levels of malondialdehyde (MDA) and testicular histopathology in rat (Rattus norvegicus) DMT2 model. The study was divided into three groups: normal without treatment, DMT2 with a 64-day high-fat feed intake of 40 g / rat / day and streptozotocin (STZ) at 30 mg / kg / week for 2 weeks, and DMT2 with induction of High-fat feed as intake of 40 g / rat / day and streptozotocin (STZ) at a dose of 30 mg / kg BB week for 2 weeks physical exercise. Physical exercise with speed and duration increased 2 weeks in 10 m / min during 10 minutes, 14 m / min for 22 minutes, 18 m / min for 34 minutes, 22 m / min for 46 minutes, and 27 m / min for 60 minutes. MDA levels were tested using Spektofotometric. MDA data were tested quantitatively using one way ANOVA α = 5% test followed by LSD, while testicular histopathology was described in qualitatively. The histopathologic picture of the testis was stained with Hematoxylin Eosin (HE) staining. The result showed that physical exercise (treadmill) decreased MDA level significantly (p<0,05), improved sertoli and leydig and there are no was able to space in semineferus tubules. The conclussion is the physical exercise (treadmill) decrease MDA level and repair histopathology of testes in rat model of DMT2.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FKH/2017/158/051709503 |
| Uncontrolled Keywords: | DMT2, Histopatologi Testis, Latihan Fisik, MDA |
| Subjects: | 600 Technology (Applied sciences) > 616 Diseases > 616.4 Diseases of endocrine, hematopoietic, lymphatic, glandular system; diseases of male breast > 616.46 Diseases of islands of Langerhans > 616.462 Diabetes mellitus |
| Divisions: | Fakultas Kedokteran Hewan > Kedokteran Hewan |
| Depositing User: | Yusuf Dwi N. |
| Date Deposited: | 19 Oct 2017 02:21 |
| Last Modified: | 07 Oct 2020 03:31 |
| URI: | http://repository.ub.ac.id/id/eprint/3999 |
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