Putra, Danny Govindra (2017) Pengaruh Pemberian Air Minum Dari Pipa Polyvinyl Chloride (PVC) Yang Terlapis Pb Terhadap Kadar Malondialdehida (MDA) Dan Gambaran Histopatologi Cerebrum Pada Tikus Putih (Rattus norvegicus). Sarjana thesis, Universitas Brawijaya.
Abstract
Plumbum (Pb) merupakan logam berat yang menjadi bahan dari pipa Polyvinyl chloride (PVC) sebagai lapisan tahan korosi. Polyvinyl Chloride (PVC) merupakan plastik yang banyak digunakan untuk pembuatan pipa air. Air minum yang disalurkan melewati pipa berlapis Pb akan menyebabkan Pb yang terlarut dalam air. Keberadaan Pb di dalam tubuh karena meminum air yang mengandung Pb menyebabkan berbagai kerusakan sel, organ, atau jaringan terutama pada cerebrum. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian air yang mengandung Plumbum (Pb) dari beberapa pipa PVC terhadap kadar Malondialdehida (MDA) dan gambaran histopatologi cerebrum pada tikus putih (Rattus norvegicus). Penelitian ini dibagi dalam 5 kelompok perlakuan yaitu kontrol negatif, kontrol positif diinduksi Pb 5 mg/ekor/hari, kelompok 3 diinduksi air rendaman pipa PVC tipe “A” (0,00±0,00), kelompok 4 diinduksi air rendaman pipa PVC tipe “B” (0,05±0,00), dan kelompok 5 diinduksi air rendaman pipa PVC tipe “C” (0,08±0,01). Kadar MDA cerebrum dianalisa dengan metode spektofotometri, uji One Way ANOVA, dan uji Tukey (p<0,05), serta histopatologi cerebrum menggunakan pewarnaan HE yang diamati adalah sel neuron dan neuroglia. Hasil penelitian menunjukan bahwa penggunaan pipa PVC tipe “A” tidak mempengaruhi kenaikan kadar MDA dan perubahan struktur sel neuron, sedangkan pipa PVC “B” dan “C” mempengaruhi kenaikan kadar MDA dan perubahan struktur sel neuron. Kesimpulan dari penelitian ini yaitu pemberian air minum rendaman pipa PVC yang mengandung Pb dapat meningkatkan radikal bebas (MDA cerebrum) dan merusak gambaran histopatologi cerebrum berdasarkan gambaran sel neuron dan sel neuroglia.
English Abstract
Lead (Pb) were heavy metal used in manufacturing Polyvinyl chloride (PVC) pipe as coating agent against corrosion. Polyvinyl Chloride (PVC) is a plastic that usually used for water pipe. Water that went through lead pipe would cause a rise of Pb inside the water contents. If the Pb were inside the body because of drinking the water which plumbum contain it, it could cause cell, organ or tissue damage, especially on cerebral. This study is aimed to determine the effect of adding water which is containing plumbum of some PVC pipes on content level of malondialdehyde (MDA) and the description of cerebral histopathology in white rats (Rattus norvegicus). This study were conducted using 5 treatment group, those were negative control, positive control that were induced with 5 mg/rat/day of Pb, 3rd group induced with water submerged with lead PVC pipe type “A” (0,00±0,00), 4th group induced with water submerged with lead PVC pipe type “B” (0,05±0,00) and 5th group induced with water submerged with lead PVC pipe type “C” (0,08±0,01). MDA cerebrum levels were analyzed by spectrophotometric method, One Way ANOVA test, and Tukey test (P<0,05), also histopathology cerebrum using HE staining and was observed by neuron cells and neuroglia cells using a light microscope. The result of this research indicates that the use of PVC pipe type “A” did not effect the increase in MDA levels and changes in the neuron cells. But PVC pipe type “B” and “C” give an effects the increase in MDA levels and changes in the neuron cells. In conclusion from this research of Drinking Water from PVC pipe containing Pb can increase free radical (MDA cerebrum) and damage the histopathology cerebrum by the neuron cells and neuroglia cells.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FKH/2017/148/051709469 |
| Uncontrolled Keywords: | Plumbum (Pb), PVC, MDA, Cerebrum |
| Subjects: | 600 Technology (Applied sciences) > 615 Pharmacology and therapeutics > 615.9 Toxicology > 615.925 Specific inorganic poisons |
| Divisions: | Fakultas Kedokteran Hewan > Kedokteran Hewan |
| Depositing User: | Yusuf Dwi N. |
| Date Deposited: | 18 Oct 2017 07:11 |
| Last Modified: | 23 Dec 2020 04:39 |
| URI: | http://repository.ub.ac.id/id/eprint/3961 |
Actions (login required)
 |
View Item |