Hasian, Tabita (2017) Uji Efektivitas Sifat Hepatoprotektor Ekstrak Bawang Lanang (Allium sativum L.) Pada Tikus Wistar Jantan Yang Diinduksi Parasetamol (Kajian Kadar MDA, SOD, dan Histopatologi Hepar). Sarjana thesis, Universitas Brawijaya.
Abstract
Hati adalah organ yang sangat penting bagi tubuh. Penggunaan parasetamol dengan dosis yang berlebihan dapat mengganggu fungsi hati, sehingga hati tidak berfungsi sebagaimana fungsinya. Salah satu bahan alami yang dapat melindungi hati dari kerusakan adalah bawang lanang (Allium sativum L.). Ekstrak bawang lanang yang disimpan dengan pelarut polar seperti air memiliki kandungan utama S-allyl-L-Cysteines (SAC) yang memberikan efek proteksi terhadap radikal bebas dan juga sebagai hepatoprotektor. Pada penelitian ini, pengujian in-vivo ekstrak bawang lanang menggunakan desain penelitian Pre Test and Post Test Control Group Design. Pemilihan objek penelitian untuk pengelompokan dan pemberian perlakuan menggunakan metode Rancangan Acak Lengkap (RAL) dengan 7 kelompok perlakuan. Data yang diperoleh kemudian dianalisis dengan analisa ragam ANOVA (Analysis of Varian), jika berpengaruh nyata (p-value <0,05) maka dilakukan Uji Lanjut BNT dengan selang kepercayaan α = 5%. Hasil penelitian menunjukkan bahwa ekstrak air bawang lanang memiliki aktivitas antioksidan IC50 sebesar 65,074 μg/ml, dengan jumlah total fenol sebesar 544,26 mg GAE/g dan total flavonoid sebesar 218,315 mg QE/g. Pemberian ekstrak bawang lanang pada tikus wistar jantan mampu meningkatkan kadar SOD dan menurunkan kadar MDA serum darah. Pemberian ekstrak bawang lanang pada tikus wistar jantan yang telah dipapar parasetamol dosis toksik mampu menekan peningkatan kadar MDA serta mempertahankan kadar SOD didalam tubuh, serta mampu melindungi sel-sel hati dari kerusakan akibat paparan parasetamol dosis toksik
English Abstract
Liver is a very important organ for the body. The use of paracetamol with excessive dose can disrupt the function of the liver, and make the liver does not work as it should. One of the natural ingredients which can protect the liver from damage is lanang garlic (Allium sativum L.). Extract lanang garlic stored with polar solvent (ex: water) have a major content of S-allyl-L-cysteines (SAC) that give a protective effect against free radicals. In this research, in-vivo test of single bulb garlic extracts was doing with Pre Test And Post Test Control Group Design. Selection of research object for grouping and treatment was doing with Random Complete Design (RAL) with 7 groups. The data obtained are then analyzed with ANOVA (Analysis of Varian), if significant (p-value <0,05) then conducted further trials of LSD with confidence interval α = 5%. The results showed that Lanang garlic extracts had IC50 antioxidant activity of 65,074 μg/ml, with total phenolic amount 544,26 mg GAE/g, and total flavonoid amount 216,315 mg QE/g. The consumption of Lanang garlic extracts in male wistar rats was able to increase SOD levels and decrease MDA levels. Consumption of Lanang garlic extracts in male wistar rats that have been exposed by paracetamol toxic dose able to suppress the increase of MDA levels and preserve SOD levels in the body, and also able to protect liver cells from damage due to exposure of paracetamol toxic dose.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FTP/2017/613/051710070 |
| Uncontrolled Keywords: | Bawang Lanang, Hepatoprotektor, Superoksida Dismutase (SOD), Malondialdehid (MDA), Histopatologi Hepar. |
| Subjects: | 600 Technology (Applied sciences) > 615 Pharmacology and therapeutics > 615.3 Organics drugs > 615.32 Drugs derived from plants and mikroorganisms > 615.324 32 Drugs derived from spesific plant (Aloe vera) > 615.324 33 Drugs derived from spesific plant (Garlic) |
| Divisions: | Fakultas Teknologi Pertanian > Teknologi Hasil Pertanian |
| Depositing User: | Budi Wahyono Wahyono |
| Date Deposited: | 18 Oct 2017 06:46 |
| Last Modified: | 30 Dec 2020 14:34 |
| URI: | http://repository.ub.ac.id/id/eprint/3951 |
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