Ciptadi, Onky Ostrava (2017) Studi Pemberian Terapi Ekstrak Etanol Daun Ceremai (Phyllanti acidus) Terhadap Kadar MDA Dan Gambaran Histopatologi Aorta Tikus Model Hiperkolesterolemia. Sarjana thesis, Universitas Brawijaya.
Abstract
Hiperkolesterolemia adalah suatu kondisi dimana meningkatnya konsentrasi kolesterol dalam darah yang melebihi nilai normal. Konsentrasi kolesterol darah pada tikus (Rattus norvegicus) dikatakan normal apabila kadar kolesterol total 50-135 mg/dl. Radikal bebas akan terakumulasi dalam tubuh penderita hiperkolesterol. Malondialdehid (MDA) merupakan salah satu marker terjadinya kerusakan oksidatif oleh radikal bebas pada membran sel. Penelitian ini bertujuan untuk mengetahui potensi ekstrak etanol daun ceremai (Phyllanthus acidus (L.) Skeels) dalam menurunkan kadar kolesterol total dan kadar malondialdehida (MDA), serta memperbaiki kerusakan jaringan aorta pada tikus hiperkolesterolemia. Penelitian ini menggunakan hewan coba tikus putih (Rattus norvegicus) jantan umur 2 bulan dengan berat badan rata-rata 200 gram, yang dibagi menjadi 4 kelompok yaitu kelompok kontrol negatif, kelompok kontrol positif, kelompok perlakuan 3 dengan dosis terapi 150 mg/kg BB dan kelompok perlakuan 4 dengan dosis 300 mg/kg BB. Kadar MDA diukur menggunakan uji Thiobarbituric Acid (TBA) dan gambaran histopatologi aorta diamati dengan pewarnaan hemaktosilin-eosin (HE). Hasil penelitian menunjukan bahwa pemberian terapi ekstrak daun ceremai (Phyllanthus acidus (L.) Skeels) dengan dosis 150 mg/kgBB dan 300 mg/kgBB dapat menurunkan kadar malondialdehida (MDA) pada tikus (Rattus norvegicus) model hiperkolesterolemia yang secara sangat signifikan dibandingkan dengan kontrol positif sebesar 37,18% dan 59,93 (p<0,01). Pemberian ekstrak daun ceremai dapat memperbaiki gambaran histopatologi aorta tikus (Rattus norvegicus) model hiperkolesterolemia dengan adanya perbaikan jaringan aorta dan penurunan intensitas sel inflamasi. Kesimpulan dari penelitian ini adalah ekstrak daun ceremai (Phyllanthus acidus (L.) Skeels) dapat digunakan sebagai terapi preventif terhadap hiperkolestrolemia.
English Abstract
Hypercholestrolemina is increating the concentration of cholesterol in blood to exceed the normal value. The normal concentrations cholestrol in rats (Rattus norvegicus) blood 50-135 mg/dl. Free Radicals would be earned in the body sufferers of hypercholesterol. Malondialdehid (MDA) is cause of oxidative damage by free radicals in the cell membrane. Exam aims of fe this research is to know the potential ethanol extracts inducse leaves cereme (Phyllanti acidus) to the level of cholesterol and malondialdehida (MDA), well as repair the damaged of aortic tissue in hypercholesterolemia rats. The research used white male rat (Rattus norvegicus) and the body weight was 200 grams, divides into 4 groups, the negative control group, of positive control group, the thirdand fourth group treatment group 3 with a dose of therapy 150 mg/kg and 300 mg/kg for fouth group. The levels of malondialdehid (MDA) were measured using test of Thiobarbituric Acid (TBA) and using microscop for the description of histopathology aorta observed by coloring hematoxylin-eosin (HE). The results of research is showed therapy cereme leaf extract (Phyllanthus acidus) with a dose of 150 mg/300 mg/kgBB can reduce levels of malondialdehida (MDA) in hypercholestrolemia rats (Rattus norvegicus) which is very significant in amount compared to positive control of 37.18% and 59,93 (p<0.01). Giving cereme leaf extract can correct the histopathology picture of the aorta rats (Rattus norvegicus) hypercholesterolemia model with the change in network duct doves and inflammatory cell structure that is experiencing a downturn. The conclusions of this research is leaf extract cereme (Phyllanthus acidus) can be used as a preventative therapy against hypercholesterolemia.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FKH/2017/146/051709467 |
| Uncontrolled Keywords: | Aorta, Daun Ceremai, Hiperkolesterol, Malondialdehida (MDA) |
| Subjects: | 600 Technology (Applied sciences) > 615 Pharmacology and therapeutics > 615.3 Organics drugs > 615.32 Drugs derived from plants and mikroorganisms > 615.323 79 Drugs derived from spesific plant (Oxalidaceae) |
| Divisions: | Fakultas Kedokteran Hewan > Kedokteran Hewan |
| Depositing User: | Yusuf Dwi N. |
| Date Deposited: | 18 Oct 2017 06:46 |
| Last Modified: | 22 Oct 2020 14:28 |
| URI: | http://repository.ub.ac.id/id/eprint/3949 |
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