Saputro, Wahyu Gesit (2017) Pengaruh Variasi pH dan Suhu Penyimpanan Terhadap Kestabilan Aktivitas Xilanase Ekstrak Kasar Dari Trichoderma Viride Diamobilisasi Dengan Matriks Bentonit. Sarjana thesis, Universitas Brawijaya.
Abstract
Enzim xilanase merupakan enzim ekstraseluler yang memiliki kemampuan menghidrolisis xilan menjadi xilosa. Enzim xilanase bersifat induktif, sehingga untuk memperoleh produk yang maksimal, dibutuhkan suatu induser sebagai pemicu pembentukan enzim xilanase, yaitu xilan. Xilan banyak dijumpai pada limbah pertanian seperti klobot jagung dengan kandungan hemiselulosa 32%. Salah satu mikroorganisme yang dapat menghasilkan xilanase adalah kapang Trichoderma viride. Amobilisasi dilakukan untuk meningkatkan stabilitas xilanase dan meningkatkan efisiensi pemakaian enzim sehingga dapat digunakan berulang kali. Xilanase diamobilisasi menggunakan matriks bentoit dengan metode adsorpsi fisik. Suhu sangat mempengaruhi kestabilan struktur dan aktivitas enzim, perubahan pH juga dapat mempengaruhi aktivitas dan stabilitas struktur enzim. Pada penelitian ini bertujuan untuk mempelajari pengaruh suhu dan pH terhadap kestabilan aktivitas xilanase. Pengaruh tersebut diamati dengan cara mengukur aktivitas xilanase sisa setalah xilanase amobil disimpan selama penyimpanan 0, 1, 2, 3, 4, 5, 6, 7 hari pada variasi suhu (frezeer, refrigrator, 30, 50 oC) dan variasi pH (3, 4, 5, 6). Aktivitas xilanase ditentukan dengan cara mengukur kadar gula pereduksi secara spektrofotometri menggunakan reagen DNS (asam dinitrosalisilat). Hasil penelitian menunjukkan bahwa pH dan suhu berpengaruh terhadap kestabilan aktivitas xilanase amobil. Xilanase amobil pada penyimpanan pH 5 memiliki persen aktivitas sisanya sebesar 63,13%, stabil hingga hari ke 6. Sedangkan xilanase amobil pada penyimpanan suhu 50 oC memiliki persen aktivitas enzim sisa sebesar 51,40%, stabil hingga hari ke 6.
English Abstract
The enzyme xylanase is an extracellular enzyme that has the ability to hydrolyze xylan into xylose. The xylanase enzyme is inductive, so in order to obtain the maximum product, an inducer is required as a trigger for the formation of xylanase enzyme, that is xylan. Xylan is commonly found in agricultural waste such as corn cloves with 32% hemicellulose content. One of the microorganisms that can produce xylanase is Trichoderma viride mold. Immobilization is performed to improve the stability of xylanase and increase the efficiency of enzyme use so it can be used repeatedly. Xylanase is immobilized using bentonite matrix by physical adsorption method. Temperature greatly affects structural stability and enzyme activity, pH changes can also affect the activity and stability of the enzyme structure. In this study aims to study the effect of temperature and pH on the stability of xylanase activity. The effect was observed by measuring residual xylanase activity after immobilized xylanase was stored for storage of 0, 1, 2, 3, 4, 5, 6, 7 days in temperature variations (freezer, refrigerator, 30, 50 oC) and pH variation (3, 4, 5, 6). The activity of xylanase was determined by measuring the spectrophotometric reducing sugar by using the DNS reagent (dinitrosalisilic acid ). The results showed that pH and temperature had an effect on the stability of immobilized xylanase activity. The immobilized xylanase pH 5 has the remaining activity percent of 63.13%, stable until day 6. While the immobilized xylanase at 50 oC temperature storage has a residual activity percentage of 51.40%, stable to day 6.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FMIPA/2017/449/051709789 |
| Uncontrolled Keywords: | Xilanase, Amobilisasi, Kestabilan, pH, Suhu |
| Subjects: | 500 Natural sciences and mathematics > 579 Natural history of microorganisms, fungi, algae > 579.5 Fungi > 579.56 Ascomycotina ( ascomycetes) > 579.567 Pyrenomycetes > 579.567 7 Hypocreales |
| Divisions: | Fakultas Matematika dan Ilmu Pengetahuan Alam > Kimia |
| Depositing User: | Nur Cholis |
| Date Deposited: | 17 Oct 2017 04:08 |
| Last Modified: | 18 Nov 2021 04:20 |
| URI: | http://repository.ub.ac.id/id/eprint/3808 |
Preview |
Text
BAGIAN DEPAN.pdf Download (511kB) | Preview |
Preview |
Text
DAFTAR PUSTAKA.pdf Download (1MB) | Preview |
Preview |
Text
BAB 4.pdf Download (1MB) | Preview |
Preview |
Text
BAB 1.pdf Download (1MB) | Preview |
Preview |
Text
BAB 5.pdf Download (1MB) | Preview |
Preview |
Text
LAMPIRAN.pdf Download (1MB) | Preview |
Preview |
Text
BAB 2.pdf Download (1MB) | Preview |
Preview |
Text
BAB 3.pdf Download (1MB) | Preview |
Actions (login required)
 |
View Item |