Subkhi, Pandu Gumelar (2017) Efek Pemberian Ekstrak Ragi (Saccharomyces cerevisiae) Pada Tikus Putih (Rattus norvegicus) Model Sepsis Induksi Escherichia coli Berdasarkan Kadar Superoksida Dismutase (Sod) Plasma Dan Gambaran Histopatologi Aorta Abdominal. Sarjana thesis, Universitas Brawijaya.
Abstract
Sepsis merupakan suatu sindroma klinik yang ditandai dengan keberadaan mikroorganisme atau toksin mikroorganime dalam sistem sirkulasi, sehingga menyebabkan aktivasi respon inflamasi secara sistemik. Tujuan penelitian ini adalah untuk mengetahui efek pemberian ekstrak ragi (Saccharomyces cereviseae) dalam meningkatan kadar SOD plasma darah dan menurunkan nekrosis sel endotel aorta abdominal tikus putih (Rattus norvegicus) model sepsis hasil induksi Escherichia coli. Penelitian ini bersifat eksperimental menggunakan Rancangan Acak Lengkap (RAL) dan terdiri dari lima kelompok, yaitu kontrol negatif, kontrol positif (diinduksi Escherichia coli) dan tiga kelompok terapi (diinduksi Escherichia coli dan diberi terapi dengan dosis bertingkat, yaitu 10, 20, dan 30 mg/kg BB). Sepsis diinduksi dengan biakan Escherichia coli standar Mc Farland 0,5 (1,5 x 108 cfu/ml) intraperitoneal pada hari ke-8 penelitian. Terapi ekstrak ragi diberikan per-oral enam jam pasca induksi, dan dilanjutkan setiap 24 jam selama lima hari. Analisis yang digunakan dalam penelitian ini adalah analisis One Way ANOVA dengan uji lanjutan BNT 5% untuk kadar SOD plasma serta analisa deskriptif untuk gambaran histopatologi aorta abdominal. Hasil penelitian menunjukkan bahwa pemberian terapi ekstrak ragi memberikan pengaruh yang signifikan terhadap kenaikan kadar SOD plasma dan menurunkan nekrosis sel endotel aorta abdominal. Berdasarkan hasil penelitian ini, dosis terapi 30 mg/kg BB merupakan dosis optimum untuk terapi sepsis, dapat disimpulkan bahwa ekstrak ragi (Saccharomyces cereviseae) dapat digunakan sebagai terapi sepsis.
English Abstract
Sepsis is a clinical syndrome characterized by the presence of microorganisms or the toxin of microorganisms in circulatory system. The purpose of this research is to determine the effect of yeast (Saccharomyces cereviseae) extract in increasing the level of plasma SOD and histopathology of abdominal aorta of sepsis rat (Rattus norvegicus) model induced by Escherichia coli. This research is an experimental using Completely Randomized Design (CRD) and consist of five goups, negative control, possitive control (induced with Escherichia coli), and three treatment groups (induced with Escherichia coli and treated with different doses yeast extract that are, 10 mg/kg BW, 20 mg/kg BW, and 30 mg/kg BW). Sepsis induced by Escherichia coli with a dose 1,5 x 108 cfu/ml injected intraperitoneally at the eighth day of the research. Yeast extract treatment was given orally six hours post induction, and continued every 24 hours for five days. Data were analyzed using One Way ANOVA with Least Significant Difference (LSD) 5% for SOD activity and descriptive analytic for aorta's histopathological appearence. The result of this research were therapeutizing of sepsis rat with yeast extract could give significant effect of SOD activity. Besides, it also capable for decreasing the necrotic endothelial cell of abdominal aorta. The conclusion of this research, the optimum dose of yeast extract for sepsis treatment is 30 mg/kg BW. Therefore, yeast extract had potential as therapeutizing for sepsis.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FKH/2017/121/051709490 |
| Uncontrolled Keywords: | Escherishia Coli, Histopatologi aorta, Ekstrak ragi (Saccharomyces cereviseae), SOD |
| Subjects: | 600 Technology (Applied sciences) > 616 Diseases > 616.9 Other disease > 616.94 Bacterial blood diseases > 616.944 Septicemia |
| Divisions: | Fakultas Kedokteran Hewan > Kedokteran Hewan |
| Depositing User: | Yusuf Dwi N. |
| Date Deposited: | 17 Oct 2017 03:39 |
| Last Modified: | 17 Jun 2022 06:04 |
| URI: | http://repository.ub.ac.id/id/eprint/3790 |
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