Arantika, Resti Vanda (2017) Uji Biokompatibilitas Respon Imun Akut Kulit Xenograft Aseluler Yang Dikombinasi Dengan Platelet Rich Plasma (Prp) Terhadap Ekspresi Interleukin-6 (IL-6) Dan Jumlah Sel Radang Pada Daerah Implantasi Di Tikus Putih (Rattus novergicus). Sarjana thesis, Universitas Brawijaya.
Abstract
Tissue engineering merupakan salah satu metode dalam bidang ilmu kedokteran yang sangat berkembang. Tiga komponen dasar tissue engineering dari jaringan biologis yaitu sel, growth factor, dan perancah (scaffold). Pemanfaatan kulit kambing sebagai scaffold biodegradable dalam bentuk matrix accellular yang dikombinasikan dengan PRP (Platelet Rich Plasma) allograft mengandung growth factor memerlukan pegujian biokompatibilitas. Penelitian ini menggunakan metode insisi kulit dan implantasi scaffold dan PRP pada subkutan tikus. Penelitian ini terbagi menjadi empat kelompok, yaitu kontrol (tanpa perlakuan), perlakuan scaffold, perlakuan PRP, dan perlakuan scaffold kombinasi PRP. Parameter yang dianalisis yaitu ekspresi IL-6 menggunakan pewarnaan imunohistokimia (IHK) dan jumlah sel radang di jaringan subkutan tikus menggunakan preparat histopatologi dengan pewarnaan Hematoxylin-Eosin (HE). Hasil dihitung secara kuantitatif dengan program immunoratio dan Image Raster 3. Data dianalisis dengan OneWay ANOVA dan dilanjutkan dengan Uji Beda Nyata Jujur (Tukey) menggunakan SPSS 22.0 for Windows. Penelitian ini menunjukkan terjadinya peningkatan yang signifikan (p<0,05) yaitu berupa peningkatan rerata persentase area ekspresi IL-6 (23.88%; 55.06%; 38.24%; 40.48%) dan rerata jumlah sel radang (0.80 sel/lapang pandang; 4.42 sel/lapang pandang; 4.36 sel/lapang pandang; 4.02 sel/lapang pandang) di area implantasi pada setiap kelompok perlakuan. Kesimpulan dari penelitian ini yaitu kombinasi scaffold xenograft dan PRP allograft yang diimplantasikan pada subkutan kulit tikus putih dapat memicu regenerasi jaringan terhadap respon imun akut berdasarkan peningkatan ekspresi IL-6 dan jumlah sel radang.
English Abstract
Tissue engineering is the one of methods in medical science which is highly developed. The three basic components tissue engineering from biological tissue namely, cell, growth factor, and scaffold. Goat skin is used as a biodegradable scaffold in matrix accellular form combined with PRP (Platelat Rich Plasma) allograft contains growth factor to speed up the wounds healing need biocompability test. This research used skin incision and implantation scaffold and PRP in rat’s subcutant. This research divided into four groups, are the control (without treatment), treatment with scaffold, treatment with PRP allograft, and scaffold combined with PRP treatment. The analyzed parameters are IL-6 expression using immunohistochemical staining (IHC) and the number of inflammatory cells using histopathology preparations stained with Hematoxylin-eosin (HE). The results quantified by immunoratio program and Image Raster 3. Data were analyzed with One Way ANOVA and Tukey using SPSS 22.0 for Windows. This research indicates the occurrence of a significant increase (p < 0.05), shown with the mean percentages enhancement of IL-6 expression area (23.88%; 55.06%; 38.24%; 40.48%)and the mean number of inflammatory cells (0.80 cell/page; 4.42 cell/page; 4.36 cell/page; 4.02 cell/page) in the implantation area of each group treatment. The conclusions of this study is the combination of scaffold xenograft and PRP allograft as implant biomaterial in subcutaneous rat can trigger the regeneration of tissue against acute immune response based on increasd expression of IL-6 and the number of inflammatory cell.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FKH/2017/112/051709481 |
| Uncontrolled Keywords: | Tissue Engineering, Scaffold, PRP (Platelet Rich Plasma), Growth Factor |
| Subjects: | 500 Natural sciences and mathematics > 571 Physiology and related subjects > 571.5 Tissue biology and regional physiology > 571.538 Tissue culture |
| Divisions: | Fakultas Kedokteran Hewan > Kedokteran Hewan |
| Depositing User: | Yusuf Dwi N. |
| Date Deposited: | 17 Oct 2017 01:26 |
| Last Modified: | 06 Nov 2020 05:39 |
| URI: | http://repository.ub.ac.id/id/eprint/3754 |
Actions (login required)
 |
View Item |