Ermadona, Miftakhul Mahfirah (2017) Pengaruh Glucomannan Hydrolysates (Gmh) Dan Balance Activ Terhadap Peningkatan Kadar Sig A, Kadar Β-Defensins Serta Jumlah Koloni Bakteri Lactobacillus Spp Pada Bacterial Vaginosis Wanita Usia Subur. Magister thesis, Universitas Brawijaya.
Abstract
Bacterial Vaginosis (BV) adalah infeksi yang disebabkan oleh ketidakseimbangan flora normal pada vagina dimana Lactobacillus spp., penghasil hydrogen peroksidase (H2O2) digantikan oleh bakteri anaerob seperti Gardnerella vaginalis, Mobiluncus spp , Prevotella spp., Bacterides, dan Mycoplasma sp., yang umumnya terjadi pada Wanita Usia Subur (WUS). Pergantian Lactobacillus spp., ini menyebabkan penurunan H2O2 yang umumnya ditandai dengan adanya produksi secret vagina yang banyak, berwarna abu-abu hingga kuning, tipis, homogen, berbau amis (fishy odor), dan terdapat peningkatan pH. Diperkirakan 75% wanita pernah mengalami gejala ini setidaknya sekali seumur hidup. Angka kejadian BV di dunia dapat dibilang cukup tinggi. Di Indonesia angka kejadian BV di RSUD dr. Soetomo Surabaya mengalami peningkatan prevalensi selama tahun 2011-2013 yaitu BV 45%-50%. BV dapat didukung karena adanya faktor resiko antara lain wanita yang memiliki banyak pasangan atau berganti-ganti pasangan seksual, pemakaian kontrasepsi IUD, penggunaan antibiotik jangka panjang, bilas vagina (douching) dan merokok. Akibat terjadinya BV pada wanita usia subur dapat memperparah infeksi saluran genital, endometritis, infeksi pascaoperasi, dan infeksi saluran kemih dapat meningkatkan resiko penyakit Pelvic Inflamatory Deases serta meningkatkan risiko komplikasi pada penyakit menular seksual, HIV, maupun infeksi penyakit kelamin lainnya. Glucomannan Hydrolysates (GMH) yang diesktrak (sebagai polisakarida) dari tanaman Konjac. GMH telah diuji baik secara in vitro maupun in vivo dan berhasil yang ditunjukkan dengan peningkatan pertumbuhan lactobacilli atau bifidobacteria dan berkurangnya patogen. Selain GMH juga digunakan Balance Activ yang didalamnya terdapat beberapa komposisi antara lain Lactic Acid, Glycogen, Propylene Glycol, Methylhydroxypropyl Cellulose, Sodium Lactate, Water, PH 3.8 yang dapat menetralkan bau tidak sedap, cepat meredakan ketidaknyamanan dan discharge abnormal yang menjadi masalah umum akibat adanya BV. Tujuan penelitian ini untuk membuktikan pengaruh pemberian metronidazole+Glucomannan Hydrolysates (GMH), Balance Activ serta Balance Activ+Glucomannan Hydrolysates (GMH) terhadap peningkatan kadar sIg A, β-Defensins, serta jumlah koloni bakteri Lactobacillus spp pada bacterial vaginosis wanita usia subur. Rancangan penelitian menggunakan True Experimental dengan jenis penelitiannya The Randomized pretest - posttest secara in vivo yang dilakukan di Laboratorium Mikrobiologi dan Biomedik Fakultas Kedokteran Universitas Brawijaya Malang serta Laboratorium RSUD. Dr. Iskak Tulungagung. Hasil Penelitian didapatkan pada parameter sIg A kelompok terapi metronidazole mengalami peningkatan secara signifikan di bandingkan dengan kelompok terapi yang lain. Pada parameter β-Defensins kelompok terapi metronidazole+GMH lebih tinggi hasilnya dibandingkan dengan kelompok terapi yang lain. Dan pada parameter jumlah koloni bakteri Lactobacillus spp kelompok terapi metronidazole+GMH dan Balance Activ+GMH mempunyai hasil yang hampir sama dalam meningkatkan jumlah koloni bakteri Lactobacillus spp. vii Metronidazole mempunyai sifat resisten, hal ini dapat dilihat dari hasil penelitian pada hari ke 11 kadar sIg A meningkat secara signifikan dan turun secara signifikan pada hari ke 22, hal yang sama telah banyak dilaporkan bahwa kesembuhan BV dalam jangka waktu 1 bulan sesudah terapi akan kambuh kembali, akan tetapi metronidazole masih menjadi terapi standart terhadap infeksi bakteri anaerob. Balance Activ yang mengandung komposisi Lactic Acid dan glikogen diharapkan dapat menurunkan pH vagina sehingga bakteri Lactobacillus spp dapat tumbuh serta GMH merupakan prebiotik yang diharapkan dapat memberikan nutrisi pada Lactobacillus spp agar dapat tumbuh lebih baik dan menggeser bakteri patogen.
English Abstract
Bacterial Vaginosis (BV) is an infection caused by an imbalance of the normal flora in the vagina where Lactobacillus spp., Producing hydrogen peroxidase (H2O2) is replaced by anaerobic bacteria such as Gardnerella vaginalis, Mobiluncus spp, Prevotella spp., Bacterides, and Mycoplasma sp. , which generally occur in women of fertile age (WUS). Substitution Lactobacillus spp., This led to a reduction of H2O2, which is generally characterized by the production of vaginal secretions are many, are gray to yellow, thin, homogeneous, smelled fishy (fishy odor), and there is an increase in pH. It is estimated that 75% of women have experienced this symptom at least once in their lifetime. The number of BV events in the world can be quite high. In Indonesia the incidence of BV in hospitals dr. Soetomo Surabaya experienced an increase in prevalence during 2011-2013 ie BV 45%-50%. BV can be supported because of the risk factors include women who have multiple partners or changing sexual partners, use of IUD, long-term antibiotic use, rinse the vagina (douching) and smoking. As a result, the occurrence of BV in women of childbearing age may exacerbate genital tract infection, endometritis, postsurgical infections, and urinary tract infection increases the risk of Pelvic inflammatory disease and increase the risk of complications Deases on sexually transmitted diseases, HIV, and other sexually transmitted infections. Glucomannan hydrolysates (GMH) are extracted (a polysaccharide) from the Konjac plant. GMH has been tested both in vitro and in vivo and successfully demonstrated by an increase in growth of lactobacilli or bifidobacteria and pathogen reduction. In addition to GMH is also used Balance Activ in which there are several compositions, among others, Lactic Acid, Glycogen, Propylene Glycol, Methylhydroxypropyl Cellulose, Sodium Lactate, Water, pH 3.8 to neutralize odors, quickly relieve discomfort and discharge, abnormal becomes a common problem due to their BV. The purpose of this study to prove the effect of metronidazole+glucomannan hydrolysates (GMH), Balance Activ and Balance Activ+glucomannan hydrolysates (GMH) on the increased levels of SIG A, β-Defensins, as well as the number of bacterial colonies bacterial vaginosis Lactobacillus spp in women of childbearing age. True Experimental research design used with this type of study The randomized pretest - posttest in vivo were conducted at the Laboratory of Microbiology, Faculty of Medicine and Biomedical and Laboratory Brawijaya University. Hospital Dr. Iskak Tulungagung. The results of the study were obtained on the parameters of the 1 group of metronidazole therapy significantly improved compared with the other treatment groups. In the β-Defensins group the metronidazole+GMH therapy group was higher in comparison with the other treatment groups. And on parameters the number of colonies of bacteria Lactobacillus spp group therapy metronidazole+GMH and Balance Activ+GMH have almost the same results in increasing the number of bacterial colonies Lactobacillus spp. ix That metronidazole has resistance properties, this can be seen from the results of the study on day 11 the sIg A level increased significantly and fell significantly on day 22, the same has been widely reported that the healing BV within 1 month after therapy will relapse Again, but metronidazole is still a standard therapy against anaerobic bacterial infections. Balance active compositions containing Lactic Acid and glycogen are expected to lower the pH of the vagina so that the bacteria Lactobacillus spp can grow and GMH is a prebiotic which is expected to provide nutrients to the Lactobacillus spp order to be able to grow better and shift the pathogenic bacteria.
| Item Type: | Thesis (Magister) |
|---|---|
| Identification Number: | TES/615.324 64/ERM/p/2017/041709000 |
| Uncontrolled Keywords: | POLYSACCHARIDES, AMORPHOPHALLUS, LACTOBACILLUS, VAGINA - DISEASES |
| Subjects: | 600 Technology (Applied sciences) > 615 Pharmacology and therapeutics > 615.3 Organics drugs > 615.32 Drugs derived from plants and mikroorganisms > 615.323 64 Drugs derived from specific plants (Capparales) |
| Divisions: | S2/S3 > Magister Kebidanan, Fakultas Kedokteran |
| Depositing User: | Nur Cholis |
| Date Deposited: | 03 Oct 2017 03:53 |
| Last Modified: | 30 Nov 2021 07:02 |
| URI: | http://repository.ub.ac.id/id/eprint/3169 |
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