Rahmawati, Naili (2017) Pengaruh Ekstrak Air Daun Kelor (Moringa Oleifera Lamk) Terhadap Kadar Sod Dan Indeks Apoptosis Endometrium Tikus Wistar Yang Dipapar Depo Medroxyprogesterone Acetate. Magister thesis, Universitas Brawijaya.
Abstract
DMPA adalah kontrasepsi hormonal yang paling sering digunakan dan memiliki efektivitas yang tinggi. Mekanisme kerja DMPA adalah menekan produksi hormon estrogen dengan menurunkan sekresi GnRH (gonadotropin releasing hormone). Estrogen mempunyai kemampuan sebagai antioksidan yaitu upregulator ekspresi gen enzim antioksidan, terutama enzim superoksida dismutase (SOD) didalam mitokondria. DMPA dapat menurunkan kadar SOD ovarium dan menyebabkan apoptosis pada sel endometrium. Kelor (moringa oleifera Lamk) memiliki kandungan zat antioksidan yaitu polifenol yang dapat mencegah kerusakan akibat radikal bebas dan mencegah reaksi berantai peroksida lipid pada membran sel. Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak air daun kelor (moringa oleifera Lamk) terhadap kadar SOD dan indeks apoptosis endometrium Tikus Wistar yang dipapar DMPA. Rancangan penelitian yang digunakan adalah true experimental dengan pendekatan post test only control group design. Sampel yang digunakan adalah Tikus Wistar berjumlah 25 tikus, terbagi dalam 5 kelompok yaitu kelompok kontrol negatif (diinjeksi aquabides 0,2 ml dan diberi aquabides secara oral sebanyak 1 cc), kelompok kontrol positif (Dipapar DMPA 2,7 mg dan diberi aquabides secara oral), kelompok perlakuan 1 (DMPA dan ekstrak air daun kelor dosis 100 mg/kg BB), Kelompok perlakuan 2 (DMPA dan ekstrak air daun kelor dosis 150 mg/kg BB), Kelompok perlakuan 3 (DMPA dan ekstrak air daun kelor dosis 200 mg/kg BB). Tikus diadaptasi selama 7 hari dan diberi perlakuan selama 28 hari. Pengukuran kadar SOD menggunakan metode Colorymetry, sedangkan pengukuran indeks apoptosis dengan metode Immunohistokimia. Data hasil pengamatan dianalisis dengan uji Anova One Way, Uji LSD dan Uji Pearson Product Moment. Berdasarkan pada hasil analisis kadar SOD uterus dengan menggunakan uji Anova One Way dan Uji Pearson Product Moment diperoleh hasil p-value > 0,05, artinya secara statistik tidak ada pengaruh ekstrak air daun kelor dalam berbagai dosis terhadap kadar SOD uterus tikus wistar, namun secara deskriptif kelompok perlakuan 1 dan kelompok perlakuan 2 menunjukkan peningkatan kadar SOD. Penelitian ini menunjukkan terdapat perbedaan signifikan dan hubungan yang signifikan pemberian berbagai dosis ekstrak air daun kelor terhadap indeks apoptosis endometrium tikus wistar yang dipapar DMPA dan terdapat hubungan yang signifikan antara kadar SOD uterus dengan indeks apoptosis tikus wistar yang dipapar DMPA. Penelitian ini menyimpulkan bahwa pemberian ekstrak air daun kelor tidak berpengaruh pada kadar SOD uterus tikus wistar yang dipapar DMPA namun berpengaruh pada indeks apoptosis endometrium tikus wistar yang dipapar DMPA dan ada hubungan antara kadar SOD uterus dengan indeks apoptosis endometrium tikus wistar yang dipapar DMPA
English Abstract
DMPA is a hormonal contraceptive that most often used and has a high level of effectiveness. Mechanism of action of DMPA that suppress the production of estrogen by lowering GnRH (gonadotropin releasing hormone). Estrogen has the ability as a powerful antioksidan as upregulator gene expression of antioxidant enzymes, especially enzimes superoxide dismutase (SOD) in the mitokondria. DMPA can decrease SOD level ovarium and causes endometrium cell apoptosis. Moringa oleifera Lamk contains antioxidant is polifenol that prevent free radical damage and prevent the chain reaction of lipid peroxidation in cell membranes. This research aimed to understand the influence of the water extract of moringa oleifera Lamk on superoksida dismutase level and indeks apoptosis endometrium of wistar rats treated by depo medroxyprogesterone acetate. Research’s design used true experimental with a posttest only control group design approach. The sample used Wistar Rats amounted to 25, divided into 5 groups. Negative control group distilled water injection 0,2 ml and distilled water oral 1 cc, positive control (exposed to 2,7 mg DMPA and distilled water oral 1 cc), the first treatment group (DMPA and moringa oleifera Lamk water extract 100 mg/kg BW), group 2 (DMPA and moringa oleifera Lamk water extract 150 mg/kg BW), group 3 (DMPA and moringa oleifera Lamk water extract 200 mg/kg BW). Rats adapted for 7 days and were treated for 28 days. SOD level was measured with colorymetry method whereas indeks apoptosis was measured with immunohistochemistry method. Data of the observation result were analyzed using Anova One Way Test, LSD Test and Pearson Product Moment Test. Based on the result of the analysis of uterus SOD level by using Anova One Way test dan Pearson Product Moment test found the result of p-value > 0,05, meaning that statistically there no influential of moringa oleifera Lamk water extract in various dosage in the uterus SOD level, but descriptively show that group 1 and group 3 the uterus SOD level to rise. The Study show significant different and significant relationship in endometrium indeks apoptosis rats wistar were treated by DMPA and show significant relationship in uterus SOD level with endometrium indeks apoptosis rats wistar were treated by DMPA. The study concluded that administration of Moringa oleifera Lamk water extract no influence on uterus SOD level, but influence on endometrium indeks apoptosis rats wistar were treated by DMPA and show relationship in uterus SOD level with endometrium indeks apoptosis rats wistar were treated by DMPA
| Item Type: | Thesis (Magister) |
|---|---|
| Identification Number: | TES/615.323 64/RAH/p/2017/041709150 |
| Uncontrolled Keywords: | MORINGA OLEIFERA, APOPTOSIS, ENDMETRIUM, MEDROXYPROGESTERONE, SUPEROXIDE DISMUTASE |
| Subjects: | 500 Natural sciences and mathematics > 583 Magnoliopsida (Dicotyledons) > 583.6 Dilleniidae > 583.64 Capparales |
| Divisions: | S2/S3 > Magister Kebidanan, Fakultas Kedokteran |
| Depositing User: | Nur Cholis |
| Date Deposited: | 20 Sep 2017 04:03 |
| Last Modified: | 29 Nov 2021 07:31 |
| URI: | http://repository.ub.ac.id/id/eprint/2772 |
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