Nurzaky, Khoiri and Dr. Ir. Marjuki, M.Sc and Dr. Ir. Dicky Pamungkas, M.Sc (2025) Pemanfaatan Yeast Hasil Ekstraksi Limbah Bioetanol sebagai Direct Fed Microbials terhadap Ekosistem dan Produk Fermentasi Rumen secara In Vitro. Magister thesis, Universitas Brawijaya.
Abstract
Penelitian ini dilakukan untuk menganalisis pengaruh yeast sisa fermentasi molases sebagai direct fed microbials untuk ternak ruminansia. Pengujian dilakukan secara in vitro untuk menilai pengaruh yeast terhadap ternak ruminansia melalui ekosistem mikroba dan produk fermentasi rumen berupa kecernaan, produksi gas, degradasi pakan, VFA, NH3, dan ESPM. Penelitian ini dibagi menjadi dua tahapan yaitu tahap pertama penelitian berupa kultivasi sel yeast dan pembuatan yeast bubuk. Tahap kedua penelitian berupa analisis secara in vitro berupa kecernaan, produksi gas, degradasi pakan, VFA (asetat, butirat, dan propionat), NH3, ESPM, dan populasi mikroba (bakteri dan fungi). Hasil penelitian tahap pertama ditujukan untuk mendapatkan sampel yeast dari hasil kultivasi menggunakan inokulum yang berasal dari limbah bioetanol yang telah dimurnikan, yang akan digunakan untuk bahan pengujian pada tahap kedua penelitian. Hasil uji viabilitas sel pada yeast hasil pengolahan sebesar 37,79%. Hasil penelitian tahap kedua menunjukkan bahwa penambahan yeast sebagai pakan aditif pada pakan lengkap secara sangat nyata meningkatkan nilai kecernaan bahan kering, nilai kecernaan bahan organik, produksi gas, degradasi pakan, VFA, NH3, dan efisiensi sintesis protein mikroba (P<0,01). Hasil penelitian in vitro, menunjukkan nilai kecernaan bahan kering (KcBK) dan nilai kecernaan bahan organik (KcBO) tertinggi pada perlakuan P3 masing-masing sebesar 66,09% dan 66,99%. Analisis produksi gas juga menunjukkan nilai tertinggi pada perlakuan P3 untuk total produksi gas 48 jam (161,15 ml/500 mg BK), potensi produksi gas (171,24 ml/500 mg BK), dan laju produksi gas (0,057 ml/jam). Hasil keseluruhan tahapan penelitian menunjukkan hasil yang baik terkait pemanfaatan yeast sebagai bahan aditif pada pakan lengkap, serta penambahan yeast sebesar 2% memberikan hasil yang berpengaruh paling nyata terhadap produk fermentasi rumen (kecernaan, produksi gas, degradasi pakan, VFA, NH3, ESPM) serta populasi mikroba rumen (bakteri dan fungi) secara in vitro.
English Abstract
This study was conducted to analyze the the effect of yeast from molasses fermentation residue as directfed microbials for ruminant livestock. Testing was conducted in vitro to assess the effect of yeast on ruminant livestock through rumen microbial ecosystem and products of rumen fermentation such as digestibility, gas production, degradability, VFA, NH3, and MPSE. This study was divided into two phases, the first phase of research in the form of yeast cell cultivation and yeast powder production. The second phase of research in the form of in vitro analysis in the form of digestibility, gas production, degradability, VFA, NH3, MPSE, and microbial population (bacteri and fungi). The results of the first phase of research were intended to obtain yeast sample that cultivated using inoculum from purified bioethanol waste which would be used for testing materials in the second phase of research. The results of cell viability in processed yeast were 37.79%. The results of the second phase of research showed that the addition of yeast as a feed additive to complete feed significantly increased the value of dry matter digestibility, organic matter digestibility, gas production, VFA, NH3 concentration, and microbial protein synthesis efficiency (MPSE) (P <0.01). The results of the in vitro study showed the highest dry matter digestibility (DMD) and organic matter digestibility (OMD) in the P3 treatment, which were 66.09% and 66.99%,. The gas production results also showed the highest value in the p3 treatment for total gas production 48h (161.15 ml/500 mg DM), gas production potential (171.24 ml/500 mg DM), and gas production rate (0.057 ml/hour). The overall results of the research phases showed good results related to the use of yeast as an additive in complete feed, and the addition of 2% yeast gave the most significant results on the in vitro products of rumen fermentation (digestibility, gas production, degradability, VFA, NH3, and MPSE) and microbial population (bacteri and fungi)
| Item Type: | Thesis (Magister) |
|---|---|
| Identification Number: | - |
| Uncontrolled Keywords: | fermentasi, in vitro, kecernaan, produksi gas, yeast-digestibility, fermentation, gas production, in vitro, yeast |
| Divisions: | S2/S3 > Magister Ilmu Ternak, Fakultas Peternakan |
| Depositing User: | Sugeng Moelyono |
| Date Deposited: | 07 Feb 2025 07:24 |
| Last Modified: | 07 Feb 2025 07:24 |
| URI: | http://repository.ub.ac.id/id/eprint/236117 |
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