Madania, Mega Nurul and Zubaidah Ningsih A.S, S.Si., M.Phil., Ph.D. and Anastasia Fitria Devi, S.T., M.FoodSt., Ph.D (2024) Studi Permeasi dan Efek Antioksidan Nanostructured Lipid CarrierKurkumin: In Vitro, Ex Vivo, dan In Vivo. Magister thesis, Universitas Brawijaya.
Abstract
Kurkumin merupakan senyawa bioaktif dari kunyit yang memiliki manfaat farmakologis seperti antioksidan, antiinflamasi, dan antikanker. Namun, penggunaannya terbatas karena bioavailabilitas, stabilitas, dan permeasinya yang rendah. Untuk mengatasi hal ini, kurkumin dienkapsulasi dalam nanostructured lipid carrier (NLC-CUR) yang mengandung komponen zat aktif, lipid, dan surfaktan. Surfaktan dapat memengaruhi karakteristik NLC dan mekanisme pengiriman obat melalui kulit sehingga dalam penelitian ini membandikan dua jenis surfaktan yaitu surfaktan non-ionik (Tween 80) dan surfaktan kationik (CTAB). Proses pembuatan NLC menggunakan metode phase inversion temperature (PIT). Karakterisasi dari NLC yaitu morfologi, stabilitas, ukuran partikel, indeks polidispersitas, zeta potensial, dan persentase enkapsulasi. Hasilnya, NLC-CURT80 memiliki ukuran partikel lebih kecil dan distribusi lebih merata, tetapi efisiensi enkapsulasinya lebih rendah dibandingkan NLC-CUR-CTAB. Sedangkan, NLCCUR-CTAB memiliki zeta potensial yang sangat positif dan stabilitas yang lebih baik. NLC-CUR menunjukkan laju permeasi awal yang lebih cepat dibandingkan dengan kurkumin yang hanya dilarutkan dalam air (CUR-Native). Laju permeasi awal NLC-CUR-T80 114 kali lebih cepat dibandingkan dengan NLC-CUR-CTAB dan 10 kali lebih cepat dibandingkan dengan CUR-Native. Sementara itu, pada membran poliamida, NLC-CUR-T80 meningkatkan laju permeasi kurkumin hingga 20 kali lebih cepat dibandingkan dengan NLC-CUR-CTAB dan 17 kali lebih cepat dibandingkan dengan CUR-Native. Pengujian anti UV menunjukkan bahwa CURNative, NLC-CUR-T80, dan NLC-CUR-CTAB menunjukkan absorbansi dominan di UV-A (320-400 nm) dan sebagian kecil di UV-B (280-320 nm), menandakan sifat anti-UV kurkumin lebih kuat di UV-A. NLC-CUR dapat meningkatkan sifat anti-UV dan kestabilannya, melalui penolakan sinar secara fisik maupun penyerapan UV. Uji aktivitas antioksidan menunjukkan aktivitas scavenging H2O2 tertinggi pada NLC-CUR-CTAB yang kemungkinan disebabkan oleh enkapsulasi kurkumin dalam sistem NLC dapat menjaga stabilitas kurkumin. Berdasarkan uji keamanan obat, gel NLC-CUR-T80 dan gel NLC-CUR-CTAB tidak termasuk iritan menurut standar GHS, 2011 dan memiliki potensi iritasi sangat ringan menurut ISO 10993-10:2010, sehingga keduanya dinilai aman digunakan.
English Abstract
Curcumin is a bioactive compound from turmeric with pharmacological benefits such as antioxidant, anti-inflammatory, and anticancer properties. However, its use is limited due to low bioavailability, stability, and permeability. To overcome this, curcumin was encapsulated in nanostructured lipid carriers (NLC-CUR) containing active compounds, lipids, and surfactants. Surfactants can influence the characteristics of NLC and the mechanism of drug delivery through the skin, so this study compared two types of surfactants: non-ionic surfactant (Tween 80) and cationic surfactant (CTAB). The NLC was prepared using the phase inversion temperature (PIT) method. The NLC was characterized in terms of morphology, stability, particle size, polydispersity index, zeta potential, and encapsulation percentage. The results showed that NLC-CUR-T80 had a smaller particle size and more uniform distribution, but lower encapsulation efficiency compared to NLCCUR-CTAB. Meanwhile, NLC-CUR-CTAB had a highly positive zeta potential and better stability. NLC-CUR showed a faster initial permeation rate compared to curcumin dissolved in water (CUR-Native). The initial permeation rate of NLCCUR-T80 was 114 times faster than NLC-CUR-CTAB and 10 times faster than CUR-Native. Meanwhile, on a polyamide membrane, NLC-CUR-T80 increased the permeation rate of curcumin up to 20 times faster than NLC-CUR-CTAB and 17 times faster than CUR-Native. The anti-UV test showed that CUR-Native, NLCCUR-T80, and NLC-CUR-CTAB showed dominant absorbance in the UV-A (320- 400 nm) and a small part in the UV-B (280-320 nm), indicating that the anti-UV properties of curcumin were stronger in the UV-A. NLC-CUR can improve the antiUV properties and its stability, through physical light rejection and UV absorption. Antioxidant activity tests showed the highest H2O2 scavenging activity in NLCCUR-CTAB, which is possibly due to the encapsulation of curcumin in the NLC system, which can maintain the stability of curcumin. Based on the drug safety test, NLC-CUR-T80 gel and NLC-CUR-CTAB gel were not classified as irritants according to the GHS 2011 standard and had very mild irritation potential according to ISO 10993-10:2010, so both were considered safe for use.
| Item Type: | Thesis (Magister) |
|---|---|
| Identification Number: | 0424090079 |
| Uncontrolled Keywords: | Kurkumin, nanostructured lipid carrier, tween 80, cetrimonium bromida, permeasi. |
| Divisions: | S2/S3 > Magister Kimia, Fakultas MIPA |
| Depositing User: | Unnamed user with username nova |
| Date Deposited: | 04 Feb 2025 04:51 |
| Last Modified: | 04 Feb 2025 04:51 |
| URI: | http://repository.ub.ac.id/id/eprint/235551 |
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