Alam, Chairiffo Nur and Dr. Ir. Yulia Nuraini, MS. (2024) Pengaruh Pemberian Konsorsium Bakteri Indigeneous Resistan Kadmium (Cd) dan Pupuk Organik Terhadap Total Populasi Bakteri Resistan Cd, dan Respirasi Tanah Serta Pertumbuhan Tanaman Kangkung Pada Tanah Tercemar Kadmium. Sarjana thesis, Universitas Brawijaya.
Abstract
Kadmium (Cd) adalah salah satu unsur logam berat yang berpotensi mencemari lingkungan. Munculnya permasalahan residu kadmium dalam produk pertanian dengan kadar 0,02-0,3 ppm akibat penggunaan bahan agrokimia dalam tanah dapat diatasi dengan bioremediasi lahan. Penelitian mengenai uji efektivitas dari bakteri indigeneous resistan kadmium yang dikonsorsiumkan untuk meningkatkan respirasi tanah dan populasi bakteri pada tanah tercemar kadmium menjadi terobosan baru di bidang pertanian guna mengatasi masalah dampak dari cemaran kadmium pada tanah dan sebagai upaya peningkatan hasil kegiatan budidaya. Tujuan dari penelitian ini yaitu: (1) menganalisis efektivitas konsorsium bakteri indegenous resistan kadmium dalam respirasi tanah dan aktivitas bakteri. (2) menguji kemampuan konsorsium bakteri indegenous resistan kadmium dalam meningkatkan pertumbuhan tumbuhan pada tanah tercemar kadmium. Penelitian dilakukan pada bulan Desember 2023 hingga bulan Maret 2024 di Laboratorium Kimia Tanah dan Biologi Tanah, serta green house Lahan Percobaan Jatimulyo, Fakultas Pertanian Universitas Brawijaya. Konsorsium bakteri indegenous terdiri B1, B6, dan B12. Metode yang digunakan dalam penelitian ini yaitu Rancangan Acak Lengkap (RAL) dengan 7 perlakuaan dan 4 kali ulangan. Perlakukan penelitian yaitu perlakuan (1) K (Kontrol), (2) TB1 (5 mL konsorsium bakteri resistan Cd), (3) TB2 (10 mL konsorsium bakteri resistan Cd), (4) TO1 (10 ton/ha atau 125 g/polybag pupuk organik), (5) TO2 (Pemberian 20 ton/ha atau 250 g/polybag pupuk organik), (6) TBO1 (5 mL konsorsium bakteri + 125 g/polybag pupuk kompos) dan (7) TBO2 (10 mL konsorsium bakteri + 250 g/polybag pupuk organik). Respirasi tanah, total populasi bakteri reistan Cd, N total tanah, pH tanah, C-organik, tinggi tanaman, jumlah daun. Analisis data menggunakan Microsoft Excel 2010, R Studio, data pengamatan yang diperoleh dilakukan uji F 5%, uji BNJ 5%, uji korelasi (r), dan uji regresi (R2). Hasil penelitian menunjukan pemberian konsorsium bakteri indegenous resistan kadmium dan pupuk organik berpengaruh nyata terhadap respirasi tanah, perlakuan yang paling tinggi yaitu perlakuan TBO2 (10 mL konsorsium bakteri dan 20 ton/ha pupuk organik) dengan rerata 35,14 mg CO2 hari-1 dan peningkatan terhadap kontrol sebesar 18,07%. Pemberian konsorsium bakteri indegenous resistan kadmium dan pupuk organik berpengaruh nyata terhadap total populasi bakteri resistan Cd dengan perlakuan tertinggi ada pada TBO2 dengan rerata populasi bakteri sebanyak 56,6 x 109 (CFU/g). Pemberian konsorsium bakteri indigenous resistan kadmium dan pupuk organik tidak berpengaruh nyata dalam meningkatkan pertumbuhan tanaman, pertumbuhan tinggi tanaman kangkung pada perlakuan TB2 (5 MST) dengan rerata tinggi tanaman 36,33 cm sedangkan pertumbuhan jumlah daun tanaman kangkung pada perlakuan TB2 memiliki rerata banyak helai daun 13 helai.
English Abstract
Cadmium (Cd) is one of the heavy metal elements that has the potential to pollute the environment. The problem of cadmium residue due to the use of chemical fertilizers and pesticides in soil can be addressed through land bioremediation. Research on the effectiveness test of cadmium-resistant indigenous bacteria consortia to improve soil respiration and bacterial populations in cadmiumcontaminated soil represents a new breakthrough in the agricultural field to overcome the impacts of cadmium contamination in soil and as an effort to increase cultivation yields. The objectives of this research are: (1) to analyze the effectiveness of cadmium-resistant indigenous bacterial consortia in soil respiration and bacterial activity, and (2) to test the ability of cadmium-resistant indigenous bacterial consortia in improving plant growth in cadmium-contaminated soil. The research was conducted from December 2023 to March 2024 at the Soil Chemistry and Soil Biology Laboratory, as well as the Jatimulyo Experimental Field greenhouse, Faculty of Agriculture, Brawijaya University. The indigenous bacterial consortium consisted of B1, B6, and B12. The method used in this research was a Completely Randomized Design (CRD) with 7 treatments and 4 replications. The research treatments were: (1) K (Control), (2) TB1 (5 mL of cadmium-resistant bacterial consortium), (3) TB2 (10 mL of cadmium-resistant bacterial consortium), (4) TO1 (10 tons/ha or 125 g/polybag of organic fertilizer), (5) TO2 (20 tons/ha or 250 g/polybag of organic fertilizer), (6) TBO1 (5 mL of bacterial consortium + 125 g/polybag of compost fertilizer), and (7) TBO2 (10 mL of bacterial consortium + 250 g/polybag of organic fertilizer). The parameters measured were soil respiration, total population of cadmium-resistant bacteria, total N in the soil, soil pH, organic C, plant height, and leaf count. Data analysis was performed using Microsoft Excel 2010 and R Studio, with the obtained data subjected to an F-test at 5%, HSD test at 5%, correlation test (r), and regression test (R²). The results showed that the application of cadmium-resistant indigenous bacterial consortia and organic fertilizers had a significant effect on soil respiration, with the highest treatment being TBO2 (10 mL of bacterial consortium and 20 tons/ha of organic fertilizer), with an average of 35.14 mg CO₂ day⁻¹ and an increase of 18.07% compared to the control. The application of cadmium-resistant indigenous bacterial consortia and organic fertilizers also had a significant effect on the total population of cadmium-resistant bacteria, with the highest treatment in TBO2, with an average bacterial population of 56.6 x 10⁹ CFU/g. However, the application of cadmium-resistant indigenous bacterial consortia and organic fertilizers did not significantly affect plant growth. The highest plant height for water spinach was recorded in the TB2 treatment (5 WAT) with an average plant height of 36.33 cm, while the highest leaf count was also recorded in TB2 with an average of 13 leaves.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | 0524040572 |
| Divisions: | Fakultas Pertanian > Ilmu Tanah |
| Depositing User: | Unnamed user with username nova |
| Date Deposited: | 14 Feb 2025 03:53 |
| Last Modified: | 14 Feb 2025 03:53 |
| URI: | http://repository.ub.ac.id/id/eprint/233184 |
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