Prayoga, Caecillia Nanda and Prof. Irnia Nurika, STP, MP, Ph.D and Prof. Ir. Nur Hidayat, MP (2024) Pengaruh Penambahan Sukrosa Dan Yeast extract Terhadap Peningkatan Yield Lipid Pada Delignifikasi Limbah Tandan Kosong Kelapa Sawit (TKKS) Menggunakan Jamur Schizophyllum Commune. Sarjana thesis, Universitas Brawijaya.
Abstract
Tandan kosong kelapa sawit (TKKS) menjadi limbah utama dari industri pengolahan kelapa sawit. Limbah TKKS memiliki komponen penyusunnya yaitu material yang kaya unsur karbon antara lain selulosa 42,7%, hemiselulosa 27,3% dan lignin 17,2%. Kandungan TKKS yang kaya akan lignoselulosa memiliki potensi untuk digunakan sebagai bahan baku dalam produksi salah satu jenis biomassa, yaitu biolipid. Keunggulan produk biolipid dari mikroorganisme yaitu siklus hidup yang pendek dan tidak membutuhkan luas lahan untuk proses produksinya.. Produksi lipid dapat dipengaruhi oleh beberapa faktor yaitu suhu, pH, induser dan C/N rasio. Faktor utama yang digunakan dalam produksi peningkatan yield lipid yaitu sumber karbon jenis sukrosa dan sumber nitrogen jenis yeast extract. Penelitian ini dilakukan dengan metode Rancangan Acak Kelompok (RAK) yang terdiri dari percobaan dua faktor. Faktor penambahan sukrosa dan yeast extract masing�masingnya terdiri dari 3 level. Pada penelitian ini dilakukan beberapa pengujian meliputi Total Soluble Phenol (TSP), Total Gula Reduksi (TGR), pH, susut berat, lignoselulosa dan yield lipid. Hasil penelitian menunjukkan bahwa jamur pendegradasi lignin Schizophyllum commune dengan penambahan sukrosa dan yeast extract terbukti mampu meningkatkan hasil degradasi lignoselulosa TKKS. Diperoleh nilai susut berat tertinggi sejumlah 29% dari penambahan sukrosa 30 g/L dan yeast extract 1 g/L. Nilai TGR tertinggi sejumlah 62 mg/g diepoleh dari penambahan sukrosa 30 g/L dan yeast extract 0,5 g/L. Nilai TSP tertinggi diperoleh dari penambahan TSP sejumlah 0,23 mg/g. Nilai pH seluruh sampel mengalami penurunan. Perlakuan terbaik pada penelitian ini, yaitu yield lipid diperoleh nilai tertinggi sejumlah 2,75% dengan penambahan sukrosa 30 g/L dan yeast extract 0,75 g/L dan perlakuan tersebut sebagai perlakuan terbaik. Diperoleh kandungan degradasi lignin tertinggi sejumlah 21,96% diperoleh dari sampel kontrol TKKS
English Abstract
Empty palm oil bunches (TKKS) are the main waste from the palm oil processing industry. TKKS waste has its constituent components, namely materials rich in carbon elements, including 42.7% cellulose, 27.3% hemicellulose and 17.2% lignin. The EFB content which is rich in lignocellulose has the potential to be used as a raw material in the production of one type of biomass, namely biolipids. The advantage of biolipid products from microorganisms is that they have a short life cycle and do not require a large area of land for the production process. Lipid production can be influenced by several factors, namely temperature, pH, inducer and C/N ratio. The main factors used in the production of increased lipid yield are a carbon source such as sucrose and a nitrogen source such as yeast extract. This research was conducted using a Randomized Block Design (RAK) method which consisted of a two-factor experiment. The factors for adding sucrose and yeast extract each consist of 3 levels. In this research, several tests were carried out including Total Soluble Phenol (TSP), Total Reducing Sugar (TGR), pH, weight loss, lignocellulose and lipid yield. The research results showed that the lignin-degrading fungus Schizophyllum commune with the addition of sucrose and yeast extract was proven to be able to increase the yield of EFB lignocellulose degradation. The highest weight loss value of 29% was obtained from the addition of 30 g/L sucrose and 1 g/L yeast extract. The highest TGR value of 62 mg/g was obtained from the addition of 30 g/L sucrose and 0.5 g/L yeast extract. The highest TSP value was obtained from the addition of 0.23 mg/g TSP. The pH value of all samples decreased. The best treatment in this research, namely lipid yield, obtained the highest value of 2.75% with the addition of 30 g/L sucrose and 0.75 g/L yeast extract and this treatment was the best treatment. The highest lignin degradation content of 21.96% was obtained from the TKKS control sample
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | 052410 |
| Uncontrolled Keywords: | Delignifikasi, Lipid, Schizophyllum commune, Tandan Kosong Kelapa Sawit (TKKS) -Delignification, Lipids, Schizophyllum commune, Palm Oil Empty Bunches (TKKS |
| Divisions: | Fakultas Pertanian > Sosial Ekonomi Pertanian |
| Depositing User: | Sugeng Moelyono |
| Date Deposited: | 14 Nov 2024 03:42 |
| Last Modified: | 14 Nov 2024 03:42 |
| URI: | http://repository.ub.ac.id/id/eprint/228619 |
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