Pamungkas, Bayu Aji and Dr. Ir. Abdul Latief Abadi, MS. (2024) Potensi Bakteri Endofit Akar Tanaman Platycerium elephantotis Sebagai Promotor Pertumbuhan Tanaman Bawang Merah (Allium ascalonicum). Magister thesis, Universitas Brawijaya.
Abstract
Bawang merah (Allium ascalonicum) sangat diminati di Indonesia, namun produksi dalam negeri tidak mencukupi, menyebabkan peningkatan impor. Hama, penyakit, tanah masam, dan perubahan iklim menghambat produksi, sering diatasi dengan pestisida sintetik yang berdampak negatif. Bakteri endofit dari tanaman epifit Platycerium elephantotis, yang dapat meningkatkan ketahanan dan pertumbuhan tanaman, menawarkan solusi potensial. Penelitian ini bertujuan mengidentifikasi potensi bakteri endofit dari P. elephantotis sebagai Plant Growth Promoting Bacteria (PGPB) untuk meningkatkan pertumbuhan dan ketahanan bawang merah. Penelitian dilakukan mulai bulan November 2023-Juli 2024. Pengambilan sampel tanaman P. elephantotis var. superbum dari Taman Konservasi Arjuna Park, Malang. Isolasi bakteri endofit dilakukan di Laboratorium Penyakit Tumbuhan, Universitas Brawijaya. Uji hipersensitif dilakukan pada tanaman tembakau dan analisis molekuler menggunakan amplifikasi DNA target 16sRNA. Potensi bakteri sebagai Plant Growth Promoting Bacteria (PGPB) diuji melalui pelarutan fosfat, penambatan nitrogen, produksi IAA, dan uji antagonis terhadap jamur F. oxysporum. Uji cekaman asam dan kering serta uji pertumbuhan in vivo pada bawang merah dilakukan di Desa Dadaprejo, Junrejo. Pengamatan organisme pengganggu tanaman (OPT) dan analisis biokimia tanaman meliputi kandungan klorofil, karotenoid, antosianin, enzim PO, dan total fenolik. Data dianalisis dengan ANOVA dan uji lanjut BNT menggunakan Microsoft Excel 2019. Hasil in vitro mendapatkan 6 isolat bakteri yang berpotensi sebagai PGPB diantaranya Bacillus cereus PE 5, Bacillus thuringiensis PE 7, Bacillus paramycoides PE 24, Stenotrophomonas maltophilia PE 25, Bacillus cereus PE 30, dan Acinetobacter nosocomonalis PE 49. Berdasarkan hasil uji in vivo, isolat bakteri B. cereus PE 5, merupakan isolat terbaik dalam meningkatkan produksi dan ketahanan tanaman bawang merah.
English Abstract
Shallots (Allium ascalonicum) are in high demand in Indonesia, but domestic production is insufficient, leading to increased imports. Pests, diseases, acidic soil, and climate change hinder production, often addressed with synthetic pesticides that have negative impacts. Endophytic bacteria from the epiphytic plant Platycerium elephantotis, which can enhance plant resilience and growth, offer a potential solution. This study aims to identify the potential of endophytic bacteria from P. elephantotis as Plant Growth Promoting Bacteria (PGPB) to improve the growth and resilience of shallots. The research was conducted from November 2023 to July 2024. Samples of P. elephantotis var. superbum were collected from Arjuna Park Conservation Garden, Malang. Endophytic bacteria isolation was carried out in the Plant Disease Laboratory, Universitas Brawijaya. Hypersensitivity tests were conducted on tobacco plants and molecular analysis used 16sRNA target DNA amplification. The potential of bacteria as Plant Growth Promoting Bacteria (PGPB) was tested through phosphate solubilization, nitrogen fixation, IAA production, and antagonistic tests against F. oxysporum fungi. Acid and drought stress tests, as well as in vivo growth tests on shallots, were conducted in Dadaprejo Village, Junrejo. Observations of pests (OPT) and biochemical analysis of plants included chlorophyll, carotenoid, anthocyanin, PO enzyme, and total phenolics content. Data were analyzed using ANOVA and further BNT tests with Microsoft Excel 2019. In vitro results identified six bacterial isolates with potential as PGPB, including Bacillus cereus PE 5, Bacillus thuringiensis PE 7, Bacillus paramycoides PE 24, Stenotrophomonas maltophilia PE 25, Bacillus cereus PE 30, and Acinetobacter nosocomonalis PE 49. Based on in vivo tests, the B. cereus PE 5 isolate was the best in improving the production and resilience of shallot plants.
| Item Type: | Thesis (Magister) |
|---|---|
| Identification Number: | 042405 |
| Divisions: | S2/S3 > Magister Ilmu Tanaman, Fakultas Pertanian |
| Depositing User: | Unnamed user with username nova |
| Date Deposited: | 04 Oct 2024 01:43 |
| Last Modified: | 04 Oct 2024 01:43 |
| URI: | http://repository.ub.ac.id/id/eprint/227535 |
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