Ratnawati, Dian and . Prof. Dr. Ir. Trinil Susilawati, MS., ASEAN Eng., and Prof. Dr. Ir. Gatot Ciptadi, DESS., IPU., ASEAN Eng. and Prof. Dr. Dra. Sri Rahayu, M.Kes (2024) Keberhasilan Pemanfaatan Ekstrak Kedelai Nanopartikel Dalam Melindungi Spermatozoa Pada Proses Pendinginan Dan Pembekuan Pada Sapi Bali. Doktor thesis, Universitas Brawijaya.
Abstract
Penelitian ini bertujuan untuk mengkaji formula pengencer CEP dan ekstrak kedelai nanopartikel (223,1 nm) terhadap kualitas, karakter motilitas dan integritas DNA spermatozoa pada semen cair dan semen beku sapi Bali. Materi penelitian menggunakan 2 ekor sapi Bali. Metode penelitian secara experimental dengan Rancangan Acak Kelompok (RAK), dengan 13 ulangan (semen cair) dan 12 ulangan (semen beku). Perlakuan formula semen cair: P1 (CEP + KT 10%), P2 (CEP + EKN 40%), P3 (CEP + EKN 50%), dan P4 (CEP + EKN 60%). Perlakuan formula semen beku: P1 (CEP + KT 10%), P2 (CEP + EKN 50%), dan P3 (CEP + EKN 60%). Parameter yang diamati diantaranya: motilitas, viabilitas, abnormalitas, membran plasma utuh, SOD, MDA, DNA Fragmentasi dan SEM. Data motilitas, viabilitas, abnormalitas, MPU dianalisis dengan menggunakan ANOVA (SPSS 16). Data SOD, MDA, dan DNA Fragmentasi dianalisis dengan menggunakan Microsoft excel dan SEM disajikan secara deskriptif. Hasil penelitian semen cair menunjukkan abnormalitas P4 (18,81%) lebih tinggi (p<0,05) daripada P1 (9,13%), P2 (9,25%), dan P3 (10,38%). Motilitas progresif pada simpan dingin hari ke-9 pada pengencer P3 (42,94%) dan P4 (47,38%) lebih tinggi (p<0,05) daripada P1 (27,72%) dan P2 (31,44%). Kadar SOD dan MDA pada hari ke-7 simpan dingin pada P1 (2,92 U/ml; 11,46 μM), P2 (0,11 U/ml; 28,14 μM), P3 (2,82 U/ml; 14,52 μM), dan P4 (3,34 U/ml; 5, 29 μM). Tingkat fragmentasi DNA pada level rendah pada semua perlakuan: P1 (3,65%), P2 (5,35%), P3 (4,15%), dan P4 (4,85%). Hasil penelitian semen beku post thawing menunjukkan viabilitas spermatozoa pada kelompok P1 (72,21%) lebih tinggi (p<0,05) daripada P2 (62,04%) dan P3 (58,83%). Nilai abnormalitas spermatozoa pada kelompok P2 (8,88%) dan P3 (10,04%) lebih tinggi (p<0,05) dari nilai rataan P1 (6,71%). Motilitas progresif spermatozoa post thawing pada P1 (65,41%) lebih tinggi (p<0,05) daripada P2 (51,36%) dan P3 (45,07%). Kadar SOD dan MDA spermatozoa post thawing pada P1 (1,14 U/ml; 12,14 μM), P2 (3,36 U/ml; 11,48 μM), dan P3 (3,08 U/ml; 6,68 μM). Tingkat fragmentasi DNA pada P1 (17,2%) lebih rendah daripada P2 (41,0%) dan P3 (39,2%). Formula pengencer CEP dan ekstrak kedelai nanopartikel (50% dan 60%) dapat mendukung kualitas spermatozoa semen cair sapi Bali dengan daya simpan sampai dengan hari ke-9. Formula pengencer CEP dan kuning telur 10% pada semen beku menghasilkan kualitas spermatozoa yang lebih baik daripada pengencer CEP dan ekstrak kedelai nanopartikel 50% dan 60% (motilitas, viabilitas, abnormalitas, fragmentasi DNA).
English Abstract
This study aims to examine the CEP diluent formula and nanoparticle soybean extract nanopartikel (223,1 nm) on the quality, motility characteristics and DNA integrity of spermatozoa in liquid and frozen semen of Bali cattle. The research material used 2 Balinese bulls. The research method was experimental using a Randomized Block Design, with 13 replications (liquid semen) and 12 replications (frozen semen). Liquid semen formula treatment: P1 (CEP + KT 10%), P2 (CEP + EKN 40%), P3 (CEP + EKN 50%), and P4 (CEP + EKN 60%). Frozen semen formula treatment: P1 (CEP + KT 10%), P2 (CEP + EKN 50%), and P3 (CEP + EKN 60%). The parameters observed were: spermatozoa motility, viability, abnormality, intact plasma membrane, SOD, MDA, DNA fragmentation and SEM. Data of motility, viability, abnormality, MPU were analyzed using ANOVA (SPSS 16). Data of SOD, MDA, and DNA Fragmentation were analyzed using Microsoft Excel and SEM presented descriptively. The results of the liquid semen study showed that P4 abnormalities (18.81%) were higher (p<0.05) compared to P1 (9.13%), P2 (9.25%), and P3 (10.38%). Progressive motility on the 9th day of cold storage in diluents P3 (42.94%) and P4 (47.38%) was higher (p<0.05) than P1 (27.72%) and P2 (31.44% ). Level of SOD and MDA on the 7th day of cold storage at P1 (2.92 U/ml; 11.46 μM), P2 (0.11 U/ml; 28.14 μM), P3 (2.82 U/ml ; 14.52 μM), and P4 (3.34 U/ml; 5, 29 μM). The level of DNA fragmentation was low in all treatments: P1 (3.65%), P2 (5.35%), P3 (4.15%), and P4 (4.85%). The results of post-thawing frozen semen research showed that spermatozoa viability in group P1 (72.21%) was higher (p<0.05) compared to P2 (62.04%) and P3 (58.83%). The spermatozoa abnormality value in groups P2 (8.88%) and P3 (10.04%) was higher (p<0.05) than the average value of P1 (6.71%). The progressive motility of post-thawing spermatozoa in P1 (65.41%) was higher (p<0.05) compared to P2 (51.36%) and P3 (45.07%). Post-thawing spermatozoa SOD and MDA levels at P1 (1.14 U/ml; 12.14 μM), P2 (3.36 U/ml; 11.48 μM), and P3 (3.08 U/ml; 6, 68 μM). The level of DNA fragmentation in P1 (17.2%) was lower than P2 (41.0%) and P3 (39.2%). The CEP diluent formula and soybean extract nanoparticles (50% and 60%) can support the quality of Bali cattle liquid semen spermatozoa with a shelf life of up to the 9th day. The CEP diluent formula and 10% egg yolk in frozen semen produced better spermatozoa quality than CEP diluent and soy nanoparticle extract 50% and 60% (motility, viability, abnormality, DNA fragmentation).
| Item Type: | Thesis (Doktor) |
|---|---|
| Identification Number: | 062405 |
| Uncontrolled Keywords: | CEP, ekstrak kedelai nanopartikel, sapi Bali |
| Divisions: | S2/S3 > Doktor Ilmu Ternak, Fakultas Peternakan |
| Depositing User: | Unnamed user with username nova |
| Date Deposited: | 06 Nov 2024 06:28 |
| Last Modified: | 06 Nov 2024 06:28 |
| URI: | http://repository.ub.ac.id/id/eprint/227192 |
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