Arwa, Hafizhah Zhafirah and Dr. Ir. Joni Kusnadi, M.Si and Siti Zaiton bt Mat So’ad, Assoc. Prof. Dr. (2024) Phytochemical Analysis and Antioxidant Activity Evaluation of Anacardium occidentale L. and Vitex pinnata L. Leaves Ethanol Extract And Fractions. Sarjana thesis, Universitas Brawijaya.
Abstract
Cashew leaves (Anacardium occidentale L.) and leban leaves (Vitex pinnata L.) are both native to Southeast Asia and have been consumed or eaten raw for years due to their beneficial components, including the phenolic compounds that possess antioxidative properties. This study aims to investigate the phytochemical composition and antioxidant properties of ethanol extract obtained from the leaves of Vitex pinnata L. and Anacardium occidentale L in Southeast Asia. The phytochemical compounds of interest, known for their antioxidant potential, include secondary metabolites such as tannins, alkaloids, phenolic compounds, flavonoids, alkaloids, steroids, triterpenoids, and cardiac glycosides. Qualitative colorimetric methods were employed for phytochemical screening, while quantitative assessments were made for total phenolic and total flavonoid content. Antioxidant activity was determined using the DPPH assay, measuring absorbance through spectrophotometry. Leaf extracts of A. occidentale and V. pinnata showed significant antioxidant activity due to their phytochemicals, excluding cardiac glycosides and alkaloids. A. occidentale had the highest total phenolic content (TPC) in the ethyl acetate fraction (353.47±3.62 mg GAE/g) and the highest total flavonoid content (TFC) also in the ethyl acetate fraction (15.39±2.23 mg QE/g). V. pinnata had the highest TPC in the methanol fraction (163.33±6.97 mg GAE/g) and the highest TFC in the methanol fraction (39.13±1.96 mg QE/g). Antioxidant activity (DPPH assay) was strongest in A. occidentale's ethyl acetate fraction (IC50: 35.83±5.11 µg/ml) and V. pinnata's methanol fraction (IC50: 15.43±0.91 µg/ml). Ascorbic acid, the standard, had an IC50 of 7.11±1.48 µg/ml. For future research, modern extraction methods, compound isolation techniques, or advanced antioxidant activity assays could enhance ourunderstanding of the antioxidant potential of these plant leaves.
English Abstract
Cashew leaves (Anacardium occidentale L.) and leban leaves (Vitex pinnata L.) are both native to Southeast Asia and have been consumed or eaten raw for years due to their beneficial components, including the phenolic compounds that possess antioxidative properties. This study aims to investigate the phytochemical composition and antioxidant properties of ethanol extract obtained from the leaves of Vitex pinnata L. and Anacardium occidentale L in Southeast Asia. The phytochemical compounds of interest, known for their antioxidant potential, include secondary metabolites such as tannins, alkaloids, phenolic compounds, flavonoids, alkaloids, steroids, triterpenoids, and cardiac glycosides. Qualitative colorimetric methods were employed for phytochemical screening, while quantitative assessments were made for total phenolic and total flavonoid content. Antioxidant activity was determined using the DPPH assay, measuring absorbance through spectrophotometry. Leaf extracts of A. occidentale and V. pinnata showed significant antioxidant activity due to their phytochemicals, excluding cardiac glycosides and alkaloids. A. occidentale had the highest total phenolic content (TPC) in the ethyl acetate fraction (353.47±3.62 mg GAE/g) and the highest total flavonoid content (TFC) also in the ethyl acetate fraction (15.39±2.23 mg QE/g). V. pinnata had the highest TPC in the methanol fraction (163.33±6.97 mg GAE/g) and the highest TFC in the methanol fraction (39.13±1.96 mg QE/g). Antioxidant activity (DPPH assay) was strongest in A. occidentale's ethyl acetate fraction (IC50: 35.83±5.11 µg/ml) and V. pinnata's methanol fraction (IC50: 15.43±0.91 µg/ml). Ascorbic acid, the standard, had an IC50 of 7.11±1.48 µg/ml. For future research, modern extraction methods, compound isolation techniques, or advanced antioxidant activity assays could enhance ourunderstanding of the antioxidant potential of these plant leaves.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | 052410 |
| Uncontrolled Keywords: | Vitex pinnata L, Anacardium occidentale L, Phytochemical Screening, Antioxidant Activity |
| Divisions: | Fakultas Teknologi Pertanian > Keteknikan Pertanian |
| Depositing User: | soegeng Moelyono |
| Date Deposited: | 13 Aug 2024 06:11 |
| Last Modified: | 13 Aug 2024 06:11 |
| URI: | http://repository.ub.ac.id/id/eprint/223860 |
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