Akbar, Muhamad Jefri Trinuansa and Dr. dr. Nur Samsu, SpPD., KGH and dr. Achmad Rifai (2024) Perbandingan Efek Losartan dan Candesartan Terhadap Kadar Cystatin C Serum dan Ekspresi Receptor For Advanced Glycation End Product (RAGE) pada Ginjal Tikus Model Diabetes yang Mendapatkan Asam Rosmarinat. Magister thesis, Universitas Brawijaya.
Abstract
Pendahuluan: Nefropati diabetik merupakan salah satu komplikasi diabetes melitus dan penyebab utama penyakit ginjal stadium akhir. Asam rosmarinat dilaporkan memiliki efikasi sebagai anti peradangan dan menurunkan komponen stres oksidatif. Losartan dan candesartan telah dilaporkan mampu menghambat progresivitas dari nefropati diabetik. Tujuan: Penelitian ini bertujuan untuk mengetahui efek pemberian kombinasi asam rosmarinat dengan losartan atau candesartan terhadap kadar Cystatin C serum dan ekspresi RAGE ginjal pada tikus model diabetes. Metode: Sebanyak 16 tikus Wistar jantan umur 10-12 minggu dibagi menjadi 4 kelompok: tanpa perlakuan (KN), tikus model diabetes (KP), KP + asam rosmarinat 75 mg/kg/hari + losartan 2,5 mg/kg/hari (KP1), dan KP + asam rosmarinat 75 mg/kg/hari + candesartan 1 mg/kg/hari (KP2). Pembuatan hewan coba dilakukan dengan memberikan diet tinggi lemak selama 21 hari dan induksi streptozotocin intraperitonial dosis tunggal. 1 minggu pasca induksi streptozotocin, tikus diberikan perlakuan selama 8 minggu. Kada Cystatin C serum diukur menggunakan ELISA, ekspresi RAGE ginjal diukur menggunakan imunohistokimia. Hasil Penelitian: Kombinasi asam rosmarinat dengan losartan (1.68 ± 0.07 vs 1.96 ± 0.13; p=0.01) dan kombinasi asam rosmarinat dengan candesartan (1.62 ± 0.03 vs 1.96 ± 0.13; p=0.00) menurunkan kadar Cystain C serum secara signifikan. Kombinasi asam rosmarinat dengan losartan (5.75 ± 1.26 vs 22.50 ± 2.65; p=0.00) dan kombinasi asam rosmarinat dengan candesartan (4.75 ± 1.26 vs 22.50 ± 2.65; p=0.00) menurunkan ekspresi RAGE ginjal secara signifikan pada tikus diabetes. Kesimpulan: Kombinasi asam rosmarinat dengan losartan atau candesartan menurunkan kadar Cystatin C serum dan ekspresi RAGE ginjal pada tikus diabetes
English Abstract
Background: Diabetic nephropathy is a complication of diabetes mellitus and the main cause of end-stage renal disease. Rosmarinic acid is reported to have anti�inflammatory efficacy and reduces oxidative stress components. Losartan and candesartan have been reported to inhibit the progression of diabetic nephropathy. Aim: This study aims to determine the effect of administering a combination of rosmarinic acid with losartan or candesartan on serum Cystatin C levels and renal RAGE expression in diabetic mice models. Methods: A total of 16 male Wistar rats aged 10-12 weeks were divided into 4 groups: without treatment (KN), diabetes model rats (KP), KP + rosmarinic acid 75 mg/kg/day + losartan 2.5 mg/kg/day (KP1), and KP + rosmarinic acid 75 mg/kg/day + candesartan 1 mg/kg/day (KP2). Experimental animals were created by providing a high-fat diet for 21 days and a single dose of intraperitoneal streptozotocin induction. 1 week after streptozotocin induction, mice were treated for 8 weeks. Serum Cystatin C levels were measured using ELISA, renal RAGE expression was measured using immunohistochemistry. Results: The combination of rosmarinic acid with losartan (1.68 ± 0.07 vs 1.96 ± 0.13; p=0.01) and the combination of rosmarinic acid with candesartan (1.62 ± 0.03 vs 1.96 ± 0.13; p=0.00) significantly reduced serum Cystain C levels. The combination of rosmarinic acid with losartan (5.75 ± 1.26 vs 22.50 ± 2.65; p=0.00) and the combination of rosmarinic acid with candesartan (4.75 ± 1.26 vs 22.50 ± 2.65; p=0.00) significantly reduced renal RAGE expression in diabetic rats. Conclusion: The combination of rosmarinic acid with losartan or candesartan reduces serum Cystatin C levels and renal RAGE expression in diabetic rats.
| Item Type: | Thesis (Magister) |
|---|---|
| Identification Number: | 042406 |
| Uncontrolled Keywords: | Nefropati diabetik, asam rosmarinat, losartan, candesartan, Cystatin C, RAGE-Diabetic nephropathy, rosmarinic acid, losartan, candesartan, Cystatin C, RAGE |
| Divisions: | Profesi Kedokteran > Spesialis Ilmu Penyakit Dalam, Fakultas Kedokteran |
| Depositing User: | soegeng sugeng |
| Date Deposited: | 02 Aug 2024 03:09 |
| Last Modified: | 02 Aug 2024 03:09 |
| URI: | http://repository.ub.ac.id/id/eprint/223405 |
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