Nurindah, Amanda Safira and drh. Galuh Chandra Agustina, M.Si and drh. Yudit Oktanella, M.Si (2024) Pengaruh Lipopolisakarida (LPS) dan GnRH Antagonis terhadap Kadar Hormon Estrogen dan Ekspresi ERα Ginjal Hewan Model Hipofungsi Ovarium. Sarjana thesis, Universitas Brawijaya.
Abstract
Hipofungsi ovarium merupakan suatu kondisi dengan penurunan produktivitas reproduksi tidak hanya terjadi pada manusia tetapi juga pada hewan terutama hewan ternak yang dapat menimbulkan kerugian ekonomi. Penelitian ini bertujuan untuk mengamati kadar hormon estrogen dalam serum dan ekspresi ERα dalam ginjal pada hewan model hipofungsi ovarium yang diinjeksi Lipopolisakarida dan GnRH antagonis yakni Ganirelix acetate pada mencit. Penelitian ini menggunakan tiga kelompok perlakuan hewan coba mencit galur Swiss webster berjenis kelamin betina, usia ³4 bulan, dan berat badan 20-30 gram. Kelompok perlakuan dibagi menjadi kontrol negatif (K-), kelompok P1 dengan injeksi Lipopolisakarida 0.0115 mg/ekor, dan kelompok P2 dengan injeksi Ganirelix acetate 0.00195 mg/ekor. Kadar hormon estrogen diamati melalui metode kompetitif ELISA dan ekspresi ERα ginjal diamati melalui pewarnaan imunohistokimia. Analisa data hasil kadar hormon estrogen dan ekspresi ERα ginjal menggunakan uji One-Way ANOVA. Kemudian dilanjutkan dengan uji lanjutan Tukey HSD. Hasil dari penelitian ini menunjukan bahwa kadar hormon estrogen tidak berbeda signifikan antara kelompok kontrol negatif dengan kelompok P1 maupun P2. Kemudian, ekspresi ERα ginjal juga menunjukkan tidak berbeda signifikan antara kelompok kontrol negatif dengan kelompok P1 maupun P2. Kesimpulan dari penelitian ini adalah kedua perlakuan induksi belum mampu memberikan hasil hipofungsi ovarium pada hewan model mencit.
English Abstract
Both humans and animals, particularly livestock, can experience ovarian hypofunction, a condition marked by reduced reproductive production that can result in financial losses. This research aimed to measure serum levels of estrogen hormone and the expression of ERα in the kidneys of mice used as models for ovarian hypofunction caused by lipopolysaccharides and GnRH antagonists, specifically Ganirelix acetate. Three research groups of female Swiss Webster breed mice weighing between 20 and 30 grams at ≥ four months of age were utilized in this investigation. The treatment group was split into three groups: P1 (Lipopolysaccharide injection, 0.0115 mg/mouse); P2 (Ganirelix acetate injection, 0.00195 mg/mouse); and negative control (K-). The renal expression of ERα is evaluated using immunohistochemistry labeling, while the amounts of estrogen hormone are measured using the Competitive ELISA method. One-Way ANOVA was used to statistically analyze the data on kidney ERα expression and estrogen hormone levels followed by Tukey's HSD test. This study showed that estrogen levels were not significantly different between the negative control group and the P1 or P2 groups. Also, renal ERα expression showed negative control group results, P1 and P2 groups were not significantly different. This study concludes that both treatments have been unable to provide ovarian hypofunction results in mouse model animals.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | 0524120011 |
| Uncontrolled Keywords: | Estrogen, ERa, Lipopolisakarida, GnRH, Hipofungsi ovarium-Estrogen, ERa, Lipopolysaccharide, GnRH Antagonist, Ovarian hypofunction. |
| Divisions: | Fakultas Kedokteran Hewan > Kedokteran Hewan |
| Depositing User: | soegeng sugeng |
| Date Deposited: | 08 May 2024 05:06 |
| Last Modified: | 08 May 2024 05:06 |
| URI: | http://repository.ub.ac.id/id/eprint/218653 |
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