Ainiyati, Savira Oktavia and Dr. Husnul Khotimah, S.Si., M.Kes. and Dr. dr. I Wayan Arsana Wiyasa, Sp.OG(K)-FER (2024) Pengaruh Isolat Biji Mahoni (Swietenia macrophylla King) terhadap Ekspresi Bax dan Bcl-2 sebagai Preventif pada Embrio Zebrafish Model Diabetes Melitus Gestasional. Magister thesis, Universitas Brawijaya.
Abstract
Diabetes melitus gestasional (DMG) terjadi pada onset kehamilan. Salah satu patofisiologis DMG terjadi akibat stres oksidatif melalui peningkatan produksi ROS yang akan memicu respon inflamasi sehingga meningkatkan protein pro-apoptosis dan/atau menghambat anti-apoptosis. Biji mahoni (Swietenia macrophylla King) dapat menjadi kandidat terapi non farmakologis karena adanya kandungan senyawa aktif saponin yang memiliki keefektifan dalam mengurangi level glukosa darah. Hewan coba yang dapat dijadikan sebagai model diabetes melitus yaitu zebrafish karena memiliki perangkat biologis dan fisiologis yang mirip dengan manusia, yaitu dalam pengaturan glukosa. Adapun tujuan dari penelitian ini yaitu membuktikan pengaruh isolat biji mahoni (Swietenia macrophylla King) memiliki efek preventif terhadap ekspresi Bax dan Bcl-2 pada embrio zebrafish model DMG. Penelitian ini menggunakan metode true experimental dengan rancangan randomized post test only controlled group design. Embrio zebrafish dibagi menjadi 7 kelompok intervensi (kontrol; glukosa 3%; dan glukosa 3% + isolat biji mahoni dengan konsentrasi 1,25; 2,5; 5; 10; 20 μg/mL). Pemberian intervensi dilakukan dari 2 hingga 72 hpf, kemudian diukur ekspresi Bax dan Bcl-2 menggunakan reverse transcription quantitative polymerase chain reaction (RT-qPCR). Hasil penelitian menunjukkan pemberian isolat biji mahoni konsentrasi 1.25, 2.5, 5, 10, 20 μg/mL dapat meningkatkan ekspresi Bax sebagai marker pro-apoptosis. Adapun konsentrasi 2.5 μg/mL mengembalikan ekspresi Bax mendekati normal. Sedangakan untuk ekspresi Bcl-2 terdapat perbedaan signifikan setelah diberikan isolat biji mahoni konsentrasi 10 μg/mL dan 20 μg/mL terhadap kelompok glukosa 3%. Berdasarkan hasil rasio kedua marker tersebut, pemberian isolat biji mahoni menunjukkan hasil yang memungkinkan adanya dominasi dari gen Bax. Isolat biji mahoni memiliki efek mencegah penurunan ekspresi Bax dan peningkatan ekspresi Bcl-2 sebagai preventif apoptosis pada embrio zebrafish model DMG.
English Abstract
Gestational diabetes mellitus (GDM) occurs at the onset of pregnancy. One of the pathophysiological factors of GDM occurs due to oxidative stress through increased production of ROS that will trigger an inflammatory response thus increasing pro-apoptotic proteins and/or inhibiting anti-apoptosis. Mahogany seeds (Swietenia macrophylla King) can be a candidate for non-pharmacological therapy due to the presence active compound of saponins that have the effectiveness in reducing blood glucose levels. Experimental animals that can be used as a model of diabetes mellitus are zebrafish because they have biological and physiological devices similar to humans, one of which is in glucose regulation. So, the purpose of this study is to prove the effect of mahogany seed isolate (Swietenia macrophylla King) has a preventive effect on the expression of Bax and Bcl-2 in zebrafish embryos modeling GDM. This study used true experimental method with randomized post test only controlled group design. Zebrafish embryos were divided into 7 intervention groups (control; 3% glucose; and 3% glucose + mahogany seed isolate with concentrations of 1.25; 2.5; 5; 10; 20 μg/mL). The intervention was conducted from 2 to 72 hpf, then the expression of Bax and Bcl-2 was measured using reverse transcription quantitative polymerase chain reaction (RT-qPCR). The results showed that the administration of mahogany seed isolate at 1.25, 2.5, 5, 10, 20 μg/mL can increase Bax expression as a pro-apoptotic marker. The dose of 2.5 μg/mL can return Bax expression to near normal. While for Bcl-2 expression is significantly different after being given mahogany seeds isolate 10 μg/mL and 20 μg/mL with the 3% glucose group. The result of ratio of the two markers showed that the administration of mahogany seed isolate allowing the dominance of the Bax gene. Mahogany seed isolate has the effect of preventing a decrease in Bax expression and an increase in Bcl-2 expression as a preventive of apoptosis in zebrafish embryos modeled by GDM.
| Item Type: | Thesis (Magister) |
|---|---|
| Identification Number: | 0424060007 |
| Uncontrolled Keywords: | Diabetes melitus gestasional, Zebrafish, Swietenia macrophylla King, Bax, Bcl-2, Gestational diabetes mellitus, Zebrafish, Swietenia macrophylla King, Bax, Bcl-2 |
| Divisions: | S2/S3 > Magister Ilmu Biomedis, Fakultas Kedokteran |
| Depositing User: | Unnamed user with email dini@ub.ac.id |
| Date Deposited: | 02 Apr 2024 04:40 |
| Last Modified: | 02 Apr 2024 04:40 |
| URI: | http://repository.ub.ac.id/id/eprint/217838 |
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