Shiami., Rizku Laily and Prof. Dr. Ir. Abdul Latief Abadi,, MS. (2024) Identifikasi Patogen Penyebab Penyakit Kanker Batang Pada Tanaman Apel Tropis Di Wilayah Batu Jawa Timur Dan Pengujian Antagonis Secara In Vitro. Sarjana thesis, Universitas Brawijaya.
Abstract
Tanaman apel menjadi ikon dari kota Batu, Jawa Timur. Komoditas tersebut menjadi kunci dari perekonomian warga setempat khususnya pada bidang destinasi wisata petik apel. Produktivitas dari tanaman apel kian tahun kian menurun yang disebabkan oleh salah satu faktor berupa adanya serangan penyakit pada tanaman apel. Penelitian ini ditujukan untuk mengidentifikasi patogen penyebab kanker batang yang menyerang tanaman apel diwilayah Batu, Jawa Timur. Penelitian ini dilaksanakan pada bulan September 2022 hingga September 2023 bertempat di lahan apel wilayah Batu, Jawa Timur dan Laboratorium Penyakit Tumbuhan Departemen Hama dan Penyakit Tumbuhan, Fakultas Pertanian Universitas Brawijaya. Kegiatan penelitian ini meliputi pembuatan media PDA, isolasi patogen dan jamur antagonis, pemurnian jamur, karakterisasi jamur, species barcoding, dan uji metabolit sekunder. Data yang didapatkan diuji dengan analisis sidik ragam (ANOVA) dan hasil dari pengujian species barcoding dianalisis menggunakan analisis BLAST dan dilanjutkan dengan pembuatan pohon filogenetik. Hasil penelitian diperoleh patogen penyebab kanker batang berupa C.acutatum. Jamur antagonis berupa Trichhoderma sp. dan Gliocladium sp. yang diujikan dengan patogen diketahui mampu menghambat pertumbuhan patogen dengan nilai daya hambat tinggi >60%. Kandungan senyawa metabolit sekunder dari jamur antagonis yang diiujikan menghasilkan senyawa flavonoid, alkaloid, saponin, steroid, terpenoid.
English Abstract
Apple plant is an icon of Batu city, East Java. This commodity is the key to the local economy, especially in the field of apple picking tourism destinations. The productivity of apple plants has been decreasing year by year, which is caused by one of the factors, namely the presence of disease attacks on apple plants. This study aims to identify the pathogen that causes stem cancer that attacks apple plants in the Batu area, Malang. This research was conducted from September 2022 to September 2023 in the apple land area of Malang, East Java and the Plant Disease Laboratory of the Department of Pests and Plant Diseases, Faculty of Agriculture, Brawijaya University. The activities of this research include making PDA media, isolating pathogens and antagonistic fungi, purifying fungi, characterizing fungi, species barcoding and secondary metabolite testing. The data obtained were tested with analysis of variance (ANOVA) and the results of the species barcoding test were analyzed using BLAST analysis and followed by making a phylogenetic tree. The results of the study obtained the pathogen that causes stem cancer in the form of C.acutatum. Antagonistic fungi in the form of Trichhoderma sp. and Gliocladium sp. which were tested with pathogens were known to be able to inhibit the growth of pathogens with high inhibition values >60%. The content of secondary metabolite compounds from the antagonistic fungi tested produced flavonoids, alkaloids, saponins, steroids, terpenoids.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | 0524040168 |
| Divisions: | Fakultas Pertanian > Agroekoteknologi |
| Depositing User: | Unnamed user with username nova |
| Date Deposited: | 25 Mar 2024 03:39 |
| Last Modified: | 25 Mar 2024 03:39 |
| URI: | http://repository.ub.ac.id/id/eprint/217538 |
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