Caula, Juhi Sekar and drh. Yudit Oktanella, M. Si (2024) Efek Penambahan EGCG dalam Pengencer Semen Beku Kambing Sebagai Senyawa Antiapoptotik Terhadap Viabilitas dan Ekspresi BCL-2 Spermatozoa Kambing Post-Thawing. Sarjana thesis, Universitas Brawijaya.
Abstract
Inseminasi buatan merupakan suatu teknologi reproduksi guna mengoptimalkan kualitas pejantan. Namun demikian, IB pada ternak kambing masih terdapat kendala karena kualitas semen beku yang didapatkan sulit memenuhi standar minimal. Kualitas semen beku yang kurang memenuhi standar ditandai dengan kurangnya daya hidup spermatozoa dan peningkatan kematian spermatozoa setelah thawing. Selama proses pembekuan akan terjadi peningkatan akumulasi ROS sehingga dapat memicu penurunan viabilitas dan peningkatan apoptosis karena penurunan protein apoptosis BCL-2. Penelitian ini bertujuan untuk mengetahui efek pemberian beberapa dosis EGCG untuk mempertahankan viabilitas dan pencegahan apoptosis dengan peningkatan protein BCL-2 antiapoptosis pada spermatozoa kambing post-thawing. Sample pada penelitian ini adalah semen beku kambing kacang sebagai kontrol negatif dan beberapa dosis EGCG yang terdiri dari lima perlakuan yaitu 50 mg/dL (P1), 100 mg/dL (P2), 200 mg/dL (P3), 400 mg/dL (P4), dan 800 mg/dL (P5). Pemeriksaan viabilitas sel spermatozoa dengan menggunakan software Open CASA® sedangkan pemeriksaan ekspresi protein BCL-2 dengan teknik imunositokimia. Data hasil penelitian dianalisis dengan metode one way ANOVA dengan software Statistical Product and Service Solution (SPSS). Pada hasil, penambahan EGCG berpengaruh nyata (P<0,05) terhadap viabilitas dan ekspresi BCL-2 spermatozoa kambing post-thawing. Penambahan dosis EGCG sebanyak 100 mg/dL menunjukan hasil yang optimal untuk mempertahankan viabilitas spermatozoa dengan rataan 64,6% ± 1,51 dan ekspresi BCL-2 paling tinggi adalah dosis 800 mg/dL dengan hasil rataan 61,95% ± 21,8. Oleh karena itu dapat disimpulkan bahwa penambahan EGCG dalam semen beku kambing mampu mempertahankan viabilitas sel dan meningkatkan ekspresi BCl-2 pada spermatozoa kambing post-thawing.
English Abstract
Artificial insemination is a reproductive technology to optimize quality of male animals. However, AI in goat farming still has problems because the quality of the frozen semen obtained is difficult to meet minimum standards. Frozen semen quality that does not meet standards is characterized by a lack of spermatozoa viability and increased spermatozoa death after thawing. During the freezing process there will be an increase in ROS accumulation which can trigger a decrease in viability and an increase in apoptosis due to a decrease in the apoptotic protein BCL-2. This study aims to determine the effect of administering several doses of EGCG to maintain viability and prevent apoptosis by increasing the antiapoptotic BCL-2 protein in post-thawing goat spermatozoa. The samples in this study were frozen goat semen as a negative control and several doses of EGCG consisting of five treatments, namely 50 mg/dL (P1), 100 mg/dL (P2), 200 mg/dL (P3), 400 mg/dL (P4), and 800 mg/dL (P5). Examination of spermatozoa cell viability using Open CASA® software while examination of BCL-2 protein expression using immunocytochemical techniques. The research data were analyzed using the one way ANOVA method with Statistical Product and Service Solution (SPSS) software. In the results, the addition of EGCG had a significant effect (P<0.05) on the viability and expression of BCL-2 post-thawing goat spermatozoa. The addition of EGCG dose of 100 mg / dL showed optimal results to maintain spermatozoa viability with an average of 64.6% ± 1.51 and the highest BCL-2 expression was a dose of 800 mg / dL with an average result of 61.95% ± 21.8. Therefore, it can be concluded that the addition of EGCG to frozen goat semen is able to maintain cell viability and increase BCl-2 expression in post-thawing goat spermatozoa.
Item Type: | Thesis (Sarjana) |
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Identification Number: | 0524130046 |
Uncontrolled Keywords: | Spermatozoa kambing, Post-thawing, EGCG, BCL-2, Apoptosis, Viabilitas |
Divisions: | Fakultas Kedokteran Hewan > Kedokteran Hewan |
Depositing User: | Endang Susworini |
Date Deposited: | 04 Mar 2024 06:06 |
Last Modified: | 04 Mar 2024 06:06 |
URI: | http://repository.ub.ac.id/id/eprint/216499 |
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