Nabiladhea, Rona and Dr.Ir. Aji Sutrisno, M.Sc.Ph.D and Prof. Dr. Muhammad Taher,, BPharm., Apt. (2022) Antibacterial and Cytotoxic Activities of Silver Nanoparticle Biogenically Synthesized by Psychotria angulata Leaf Extract. Magister thesis, Universitas Brawijaya.
Abstract
Multidrug-resistant microorganisms have presently restricted the efficacy of antibacterial agents, while the treatment options for cancer, which is one of the primary causes of mortality due to human diseases, are also limited. This study aimed to reveal the antibacterial and cytotoxic activities of silver nanoparticles (AgNPs) synthesized from Psychotria angulata leaf extract. The leaves were extracted using Ultrasonic-assisted Extraction (UAE) method and phytochemically profiled with Liquid Chromatography-Mass Spectrometry (LC-MS) analysis. The obtained phytochemicals such as flavonoids, alkaloids and phenols indicate that P. angulate serve its potential as a source for AgNPs synthesis. The biosynthesized AgNPs were characterized using UV-Vis spectroscopy, Fourier transform infrared spectroscopy, Zeta potential, and SEM. The presence of AgNPs was confirmed by the UV-Vis spectra which exhibited a distinctive peak at 444.3 nm. The FTIR spectrum revealed absorption bands at various frequencies, from 404.03 to 3528.82 cm-1 that are associated with certain functional groups that contribute to the formation and stability of AgNPs. The good stability of AgNPs was indicated by the zeta potential value of -13.2 mV, while SEM confirmed the AgNPs have spherical shape with a size of ± 76.72 nm. The antibacterial activity was tested with disk diffusion and microdilution method against B. subtilis, S. aeureus, E. coli and P. aeruginosa, with a zone of inhibition at 8.83 ± 0.22, 9.33 ± 0.54, 10 ± 0.7, and 9.6 ± 0.5 mm, respectively. The cytotoxic activity was assessed through MTT assay against MCF-7 cell line that resulted in 27.43% cell viability. In conclusion, biosynthesized AgNPs from P. angulata have both antibacterial and cytotoxic properties, implying more research is needed to develop effective antibacterial and anticancer formulations.
English Abstract
Multidrug-resistant microorganisms have presently restricted the efficacy of antibacterial agents, while the treatment options for cancer, which is one of the primary causes of mortality due to human diseases, are also limited. This study aimed to reveal the antibacterial and cytotoxic activities of silver nanoparticles (AgNPs) synthesized from Psychotria angulata leaf extract. The leaves were extracted using Ultrasonic-assisted Extraction (UAE) method and phytochemically profiled with Liquid Chromatography-Mass Spectrometry (LC-MS) analysis. The obtained phytochemicals such as flavonoids, alkaloids and phenols indicate that P. angulate serve its potential as a source for AgNPs synthesis. The biosynthesized AgNPs were characterized using UV-Vis spectroscopy, Fourier transform infrared spectroscopy, Zeta potential, and SEM. The presence of AgNPs was confirmed by the UV-Vis spectra which exhibited a distinctive peak at 444.3 nm. The FTIR spectrum revealed absorption bands at various frequencies, from 404.03 to 3528.82 cm-1 that are associated with certain functional groups that contribute to the formation and stability of AgNPs. The good stability of AgNPs was indicated by the zeta potential value of -13.2 mV, while SEM confirmed the AgNPs have spherical shape with a size of ± 76.72 nm. The antibacterial activity was tested with disk diffusion and microdilution method against B. subtilis, S. aeureus, E. coli and P. aeruginosa, with a zone of inhibition at 8.83 ± 0.22, 9.33 ± 0.54, 10 ± 0.7, and 9.6 ± 0.5 mm, respectively. The cytotoxic activity was assessed through MTT assay against MCF-7 cell line that resulted in 27.43% cell viability. In conclusion, biosynthesized AgNPs from P. angulata have both antibacterial and cytotoxic properties, implying more research is needed to develop effective antibacterial and anticancer formulations.
| Item Type: | Thesis (Magister) |
|---|---|
| Identification Number: | 0422100041 |
| Uncontrolled Keywords: | Antibacterial activity, cytotoxic activity, Psychotria angulata, silver nanoparticles |
| Divisions: | S2/S3 > Magister Keteknikan Pertanian, Fakultas Teknologi Pertanian |
| Depositing User: | soegeng sugeng |
| Date Deposited: | 23 Feb 2024 06:30 |
| Last Modified: | 23 Feb 2024 06:30 |
| URI: | http://repository.ub.ac.id/id/eprint/216467 |
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