Khairunnisa, Arsila and drh. Reza Yesica, M.Sc and drh. Gretania Residiwati, M. Si., Ph.D (2024) Efek Suplementasi Air Zamzam Terhadap Diameter, Viabilitas, dan Germinal Vesicle Breakdown Rate Oosit Ikan Zebra (Danio Rerio) dengan Paparan Heat Stres. Sarjana thesis, Universitas Brawijaya.
Abstract
Suhu merupakan salah satu faktor lingkungan yang penting karena pengaruhnya terhadap metabolisme, perilaku makan, kinerja pertumbuhan, kelangsungan hidup, dan ketahanan terhadap penyakit. Heat stress dapat menekan pertumbuhan, efisiensi pakan, fungsi fisiologis, respon imun, dan status oksidatif pada organ hewan. Adanya peningkatan produksi Reactive Oxidative Stress (ROS) yang berlebihan dan menyebabkan stres oksidatif. Antioksidan diperlukan untuk menyeimbangkan kembali sel-sel yang mengalami dampak yang diakibatkan oleh stress oksidatif. Tujuan dari dilakukannya penelitian ini yaitu untuk menganalisis pengaruh suplementasi air Zamzam dengan dosis 2ml terhadap diameter, viabilitas, dan Germinal Vesicle Breakdown (GVBD) rate pada oosit ikan zebra (Danio rerio) yang dipapar heat stress. Ikan Zebra berusia 7 bulan yang telah dipelihara, dikoleksi oositnya dan diberikan perlakuan yang dibagi menjadi dua kelompok besar yaitu kelompok Non Heat Stress (NHS;28) dan Heat Stress (HS;32,34). Data dianalisis secara kuantitatif menggunakan Software Package for the Social Science (SPSS) dengan Two Way Analysis of Variant (ANOVA) (p < 0,05). Hasil uji Two Way ANOVA menunjukkan bahwa suplementasi air Zamzam memberikan pengaruh positif terhadap diameter, viabilitas dan GVBD rate. Hal ini ditunjukkan oleh signifikasi terhadap diameter oosit (p=0,004), viabilitas (p=0,002), GVBD rate (p=0,000).
English Abstract
Temperature is an important environmental factor because it influences metabolism, feeding behavior, growth performance, survival, and disease resistance. Heat stress can suppress growth, feed efficiency, physiological function, immune response, and oxidative status in animal organs. There is an increase in the excessive production of Reactive Oxidative Stress (ROS) which causes oxidative stress. Antioxidants are needed to rebalance cells that are experiencing the effects of oxidative stress. This research aims to analyze the effect of Zamzam water supplementation with a dose of 2ml on diameter, viability, and Germinal Vesicle Breakdown (GVBD) rate in zebrafish (Danio rerio) oocytes exposed to heat stress. 7-month-old zebrafish that had been reared, had their oocytes collected and given treatment which were divided into two large groups, namely the Non Heat Stress (NHS;28) and the Heat Stress (HS;32,34). Data were analyzed quantitatively using the Software Package for the Social Science (SPSS) with Two Way Analysis of Variant (ANOVA) (p < 0.05). The results of the Two Way ANOVA test showed that Zamzam water supplementation had a positive influence on diameter, viability and GVBD rate. This was shown by the significance of oocyte diameter (p=0.004), viability (p=0.002), GVBD rate (p=0.000).
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | 0524130029 |
| Uncontrolled Keywords: | Ikan Zebra, air Zamzam, heat stress, antioksidan, oosit |
| Divisions: | Fakultas Kedokteran Hewan > Kedokteran Hewan |
| Depositing User: | Endang Susworini |
| Date Deposited: | 26 Feb 2024 08:15 |
| Last Modified: | 26 Feb 2024 08:15 |
| URI: | http://repository.ub.ac.id/id/eprint/216205 |
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