Pengaruh Pemberian Kombinasi Metformin dan Lactobacillus plantarum DAD-13 Terhadap Profil Mikrobiota Tikus Model Non-Alcoholic Fatty Liver Disease (NAFLD).

Nugraha, Gilang Sotya and Dr. dr. Syifa Mustika, Sp.PD, K-GEH. FINASIM and dr. Yuanita Mulyastuti, M.Si. (2024) Pengaruh Pemberian Kombinasi Metformin dan Lactobacillus plantarum DAD-13 Terhadap Profil Mikrobiota Tikus Model Non-Alcoholic Fatty Liver Disease (NAFLD). Sarjana thesis, Universitas Brawijaya.

Abstract

Latar Belakang: Mikrobiota usus memiliki peran sentral dalam metabolisme tubuh. Disbiosis, yang merupakan suatu kondisi di mana terjadi perubahan komposisi, distribusi, dan aktivitas mikrobiota usus, memiliki kaitan erat dengan kelainan metabolisme tubuh seperti obesitas dan non-alcoholic fatty liver disease (NAFLD). Pada kasus NAFLD, ditemukan peningkatan Bacterioidetes, penurunan Firmicutes, dan peningkatan jumlah Proteobacteria yang kemudian memengaruhi produksi Short-Chain Fatty Acids (SCFA) dan ethanol. Metode: Studi ini dilaksanakan menggunakan pendekatan penelitian berupa true experimental study dengan post test only group design. Kelompok subjek penelitian dibagi menjadi tiga, yaitu kelompok western diet, kelompok diet normal, dan kelompok intervensi kombinasi metformin – Lactobacillus plantarum. Pengumpulan sampel dilakukan melalui pendekatan Bahan Biologis Tersimpan dari penelitian sebelumnya yang menerapkan metode umbrella consent. Sampel feses kemudian ditanam pada media Eosin Methylene Blue (EMB) untuk mengidentifikasi Escherichia coli dan pada media Brucella Blood Agar untuk mendeteksi Clostridium spp. Jumlah koloni dihitung (dalam satuan CFU/gram). Selanjutnya, dilakukan analisis data menggunakan uji statistik, yaitu dengan melakukan uji normalitas, uji homogenitas. uji komparasi dengan metode one way-ANOVA, dan uji Kruskal – Wallis. Hasil: Rerata jumlah koloni dari E. coli adalah pada KMP sebesar 189.324,33 ± 136.563,28, pada KDN sebesar 111.924,60 ± 98.058,55, pada KWD sebesar 315.385,71 ± 191.751,80. Sedangkan rerata jumlah koloni Clostridium pada KMP adalah sebesar 15.500 ± 11.649,64, pada KDN sebesar 7.666,67 ± 4.802,77, dan pada KWD sebesar 10.111,11 ± 3.407, 50. Uji One-Way ANOVA dari koloni Clostridium menunjukkan P-value sebesar 0,160 (p > 0,05) sementara uji Kruskal-Wallis jumlau koloni E. coli menunjukkan P-value sebesar 0,461 (P> 0,05) Kesimpulan: Penelitian ini menunjukkan peningkatan bakteri Clostridium spp. dan penurunan bakteri Escherichia coli pada kelompok tikus yang diberikan intervensi kombinasi metformin dan Lactobacillus plantarum DAD-13 yang tidak signifikan.

English Abstract

Introduction : The gut microbiota plays a central role in the body's metabolism. Dysbiosis, a condition characterized by changes in the composition, distribution, and activity of gut microbiota, is closely associated with metabolic disorders such as obesity and non-alcoholic fatty liver disease (NAFLD). In cases of NAFLD, an increase in Bacteroidetes, a decrease in Firmicutes, and an elevation in the number of Proteobacteria had been identified, subsequently affecting the production of Short-Chain Fatty Acids (SCFA) and ethanol. Methods : This study employed a true experimental design with a post-test-only group design. The research subjects were divided into three groups: a Western diet group, a normal diet group, and an intervention group receiving a combination of metformin and Lactobacillus plantarum. Sample collection utilized a Stored Biological Materials from previous study that applied the umbrella consent method. Fecal samples were then cultured on Eosin Methylene Blue (EMB) agar to identify Escherichia coli and on Brucella Blood Agar to detect Clostridium spp. Colony counts (in CFU/gram) revealed that the intervention group receiving a combination of metformin and Lactobacillus plantarum exhibited a decrease in Escherichia coli colonies compared to the Western diet group. Conversely, there was an increase in the number of Clostridium spp. in the metformin-Lactobacillus plantarum group compared to the Western diet group. Subsequently, data analysis was conducted using statistical test, including the normality test, the homogeneity test, one-way ANOVA, and the Kruskal– Wallis test. Result : The average number of E. coli colonies for the treatment groups KMP, KDN, and KWD are 189,324.33 ± 136,563.28, 111,924.60 ± 98,058.55, and 315,385.71 ± 191,751.80, respectively. On the other hand, the mean number of Clostridium colonies for the same treatment groups are 15,500 ± 11,649.64 (KMP), 7,666.67 ± 4,802.77 (KDN), and 10,111.11 ± 3,407.50 (KWD) respectively. The One-Way ANOVA test for Clostridium colonies yields a P-value of 0.160 (p > 0.05), indicating no significant difference, while the Kruskal-Wallis test for E. coli colonies shows a P-value of 0.461 (P > 0.05), also suggesting no significant difference. Conclusion : This study indicates an increase in Clostridium spp. bacteria and a decrease in Escherichia coli bacteria in the group of rats that were given the combination intervention of metformin and Lactobacillus plantarum DAD-13, although the changes observed were not statistically significant.

Item Type:	Thesis (Sarjana)
Identification Number:	0524060096
Divisions:	Fakultas Kedokteran > Pendidikan Dokter
Depositing User:	Endang Susworini
Date Deposited:	21 Feb 2024 04:10
Last Modified:	21 Feb 2024 04:10
URI:	http://repository.ub.ac.id/id/eprint/215973

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