Dewi, Annisa Bintang Dwinanda and Prof. Dr. Agustin Krisna Wardani, STP, M.Si. and Bambang Sunarko, Prof. Dr.rer.nat. (2023) Sintesis Nikotinamida dan Asam Nikotinat Melalui Biotransformasi 3-Sianopiridin Menggunakan Rhodococcus pyridinivorans strain I-Benzo. Sarjana thesis, Universitas Brawijaya.
Abstract
Nicotinamide and nicotinic acid are two forms of vitamin B3 that are important to the body because they act as precursors of the body's metabolic coenzymes. Generally, nicotinamide and nicotinic acid produced by chemical hydrolysis of 3-cyanopyridine. However, the method is considered as less environmentally friendly and high costs because it requires high pH and temperature-pressure. Therefore, the objective of this study was to test the ability of the local bacteria Rhodococcus pyridinivorans strain I-Benzo to transform 3-cyanopyridine. The research is broadly divided into 4 stages as follows: 1) testing the effect of various substrates on the growth of R. pyridinivorans strain I-Benzo; 2) determining the growth curve on selected substrates (20 mM benzonitrile; 3) testing the biotransformation of 3-cyanopyridine; and 4) optimizing the transformation reaction conditions. The results showed that R. pyridinivorans strain I-Benzo was able to use alifatic nitrile and amide (acetonitrile and acetamide) as well as aromatic nitrile (benzonitrile) as a source of carbon (C), nitrogen (N) and energy for its growth. R. pyridinivorans strain I-Benzo grew in 20 mM benzonitrile with a lag phase that ended at 12 hours and continued with an exponential phase that ended at 67 hours. R. pyridinivorans strain I-Benzo grew with doubling time (td) at 15 hours 25 minutes and specific growth rate (μ) at 0,0449/hour with cell biomass obtained was 0,8118 g/L (dry weight cell). In addition, R. pyridinivorans strain I-Benzo was able to transform 3-cyanopyridine into nicotinamide and nicotinic acid with the indication of enzymes involved are nitril-hydratase and amidase. Biotransformation of 100 mM 3-cyanopyridine produced nicotinic acid with a yield of 93% during 4 hours of reaction. The maximum activities of nitrile-hydratase and amidase respectively were obtained at 210,3 units and 28,95 units. Optimum transformation conditions can be achieved with a 3-cyanopyridine concentration of 50 mM, pH 7, temperature 40oC and cell concentration of 10 mg/ml.
English Abstract
Nicotinamide and nicotinic acid are two forms of vitamin B3 that are important to the body because they act as precursors of the body's metabolic coenzymes. Generally, nicotinamide and nicotinic acid produced by chemical hydrolysis of 3-cyanopyridine. However, the method is considered as less environmentally friendly and high costs because it requires high pH and temperature-pressure. Therefore, the objective of this study was to test the ability of the local bacteria Rhodococcus pyridinivorans strain I-Benzo to transform 3-cyanopyridine. The research is broadly divided into 4 stages as follows: 1) testing the effect of various substrates on the growth of R. pyridinivorans strain I-Benzo; 2) determining the growth curve on selected substrates (20 mM benzonitrile; 3) testing the biotransformation of 3-cyanopyridine; and 4) optimizing the transformation reaction conditions. The results showed that R. pyridinivorans strain I-Benzo was able to use alifatic nitrile and amide (acetonitrile and acetamide) as well as aromatic nitrile (benzonitrile) as a source of carbon (C), nitrogen (N) and energy for its growth. R. pyridinivorans strain I-Benzo grew in 20 mM benzonitrile with a lag phase that ended at 12 hours and continued with an exponential phase that ended at 67 hours. R. pyridinivorans strain I-Benzo grew with doubling time (td) at 15 hours 25 minutes and specific growth rate (μ) at 0,0449/hour with cell biomass obtained was 0,8118 g/L (dry weight cell). In addition, R. pyridinivorans strain I-Benzo was able to transform 3-cyanopyridine into nicotinamide and nicotinic acid with the indication of enzymes involved are nitril-hydratase and amidase. Biotransformation of 100 mM 3-cyanopyridine produced nicotinic acid with a yield of 93% during 4 hours of reaction. The maximum activities of nitrile-hydratase and amidase respectively were obtained at 210,3 units and 28,95 units. Optimum transformation conditions can be achieved with a 3-cyanopyridine concentration of 50 mM, pH 7, temperature 40oC and cell concentration of 10 mg/ml.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | 052310 |
| Uncontrolled Keywords: | Rhodococcus pyridinivorans strain I-Benzo, biotransformasi, 3-sianopiridin, nikotinamida, asam nikotinat-Rhodococcus pyridinivorans strain I-Benzo, biotransformation, 3-cyanopyridine, nicotinamide, nicotinic acid |
| Divisions: | Fakultas Teknologi Pertanian > Keteknikan Pertanian |
| Depositing User: | soegeng sugeng |
| Date Deposited: | 29 Jan 2024 03:14 |
| Last Modified: | 29 Jan 2024 03:14 |
| URI: | http://repository.ub.ac.id/id/eprint/213497 |
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