Usfahik, Yuliana Andreni and Dr. Yuli Nugraeni, drg., Sp.KG (2023) Perbedaan Jumlah Bakteri Streptococcus mutans pada Bulu Sikat Gigi Charcoal dan Non-Charcoal secara In Vitro. Sarjana thesis, Universitas Brawijaya.
Abstract
Latar belakang: sikat gigi yang umum digunakan untuk membersihkan rongga mulut dapat terkontaminasi berbagai jenis mikroorganisme sehingga memungkinkan penyebaran penyakit baik dari lingkungan maupun antar individu. Streptococcus mutans merupakan salah satu mikroorganisme yang paling banyak mengkontaminasi bulu sikat gigi dan juga merupakan bakteri utama penyebab karies serta berbagai kesehatan gigi dan mulut lainnya. Kandungan charcoal pada sikat gigi diklaim memiliki sifat antibakteri sehingga dapat mengurangi kontaminasi bakteri namun belum banyak bukti ilmiah yang mendukung klaim ini. Tujuan: mengetahui perbedaan jumlah bakteri Streptococcus mutans pada bulu sikat gigi charcoal dan non-charcoal secara in vitro. Metode: suspensi bakteri dipaparkan pada 32 sampel sikat gigi yang terdiri dari 16 sikat gigi charcoal dan 16 sikat gigi non-charcoal. Setelah 24 jam bulu sikat gigi dibilas kemudian dikultur pada media agar selama 24 jam. Penghitungan bakteri dilakukan dengan metode Total Plate Count menggunakan colony counter manual. Hasil: berdasarkan uji independent t-test p<0,005 menunjukkan perbedaan jumlah bakteri Streptococcus mutans pada bulu sikat gigi charcoal dan non-charcoal signifikan secara statistik, jumlah bakteri pada bulu sikat gigi charcoal lebih rendah dibandingkan dengan jumlah bakteri pada bulu sikat gigi non-charcoal. Kesimpulan: terdapat perbedaan jumlah bakteri Streptococcus mutans pada bulu sikat gigi charcoal dan non-charcoal.
English Abstract
Background: Toothbrushes that are commonly used to clean the oral cavity can be contaminated with various types of microorganisms, thus allowing the spread of disease both from the environment and between individuals. Streptococcus mutans is one of the microorganisms that most commonly contaminates toothbrush bristles and is also the main bacterium that causes caries and various other dental and oral health issues. The charcoal content in toothbrushes is claimed to have antibacterial properties so that it can reduce bacterial contamination, but there is not much scientific evidence to support this claim. Objective: this study aims to determine the differences of Streptococcus mutans bacterial count between charcoal and noncharcoal bristles through In Vitro. Methods: bacterial suspension was exposed to 32 toothbrush samples consisting of 16 charcoal toothbrushes and 16 noncharcoal toothbrushes. After 24 hours, the toothbrush bristles were rinsed and then cultured on agar medium for 24 hours. Bacterial counting was carried out using the Total Plate Count method using a manual colony counter. Result: based on an independent t-test p<0.005, the differences of Streptococcus mutans bacterial count between charcoal and noncharcoal bristles was statistically significant, the number of bacteria on charcoal toothbrush bristles was lower than the number of bacteria on non-charcoal toothbrush bristles. Conclusion: there were differences of Streptococcus mutans bacterial count between charcoal and noncharcoal bristles.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | 0523140003 |
| Uncontrolled Keywords: | Streptococcus mutans, charcoal, non-charcoal, bulu sikat gigi-mutans, charcoal, noncharcoal, toothbrush bristle |
| Divisions: | Fakultas Kedokteran Gigi > Kedokteran Gigi |
| Depositing User: | soegeng sugeng |
| Date Deposited: | 22 Jan 2024 04:00 |
| Last Modified: | 22 Jan 2024 04:00 |
| URI: | http://repository.ub.ac.id/id/eprint/212985 |
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Text (DALAM MASA EMBARGO)
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