NABILA EDINING, PUTRI and Dr. Widya Dwi Rukmi Putri,, STP., MP. and Dego Yusa Ali,, STP, M.Sc., Assoc. (2023) Ethnopharmacological Study and Toxicity Test of Halia Bara (Zingiber officinale Theilade) Extract Using Zebrafish (Danio rerio) Embryo. Sarjana thesis, Universitas Brawijaya.
English Abstract
Halia Bara (Zingiber officinale Theilade) is known as red ginger. Halia Bara contains bioactive compounds such as phenolics, flavonoids, alkaloids, gingerol, and shogaol. Halia Bara might be toxic because presence of dominant gingerol and 6-shogaol compounds that characterize the ginger family. These compounds are the most abundant in the Halia Bara rhizome. This research aims to determine the toxicity level of Halia Bara extract using zebrafish embryos and identify the traditional use of Halia Bara by a qualitative ethnopharmacological study referring to the Malay Manuscript "Kitab Tib Muzium Terengganu". The crude extract will be prepared by macerating the dried rhizomes with water solvent and then drying the Halia Bara using a freeze-drying machine. Halia Bara’s GC-MS (Gas Chromatography-Mass Spectrophotometry) results show 40 peaks, with geraniol as the major peak component. Halia Bara extract will divide into serial dilution concentrations (500 μg/ml, 250 μg/ml, 125 μg/ml, 62.5 μg/ml, and 31.25 μg/ml). After treatment at 24, 48, 72, and 96 hpf, the toxic effects of samples on zebrafish embryos will observe. Coagulation, lack of somite formation, non-detachment of the tail, and lack of a heartbeat are the four parameters that indicate mortality. In addition, morphological and teratogenic analyses like as heart rate, yolk size, eye size, body length, and hatching were performed. The average LC value result toxicicty study with Zebrafish Embryo Acute Test (ZFET) was 166,493 ppm. The correlation between concentrations and variables analysis was performed using Minitab 19, and the p value was derived using One Way ANOVA analysis using the Tukey's test with p 0.05 (p-value = 0,000). Therefore, the chemical exposure concentrations influence morphological and teratogenic defects in zebrafish embryos.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | 052310 |
| Uncontrolled Keywords: | Halia Bara, extraction, zebrafish embryos, GC-MS |
| Divisions: | Fakultas Teknologi Pertanian > Keteknikan Pertanian |
| Depositing User: | Unnamed user with username verry |
| Date Deposited: | 12 Jan 2024 02:03 |
| Last Modified: | 12 Jan 2024 02:03 |
| URI: | http://repository.ub.ac.id/id/eprint/208780 |
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