Husna, Safira Mustafida and drh. Yudit Oktanella, M. Si and Handayu Untari, Dr. drh. (2023) Perbandingan Kadar Glukosa dan Gambaran Histopatologi Pankreas pada Tikus (Rattus norvegicus) Model Polycystic Ovary Syndrome (PCOS) yang Diinduksi Testosteron Propionat dan Estradiol Valerat. Sarjana thesis, Universitas Brawijaya.
Abstract
Polycystic Ovary Syndrome merupakan kumpulan gejala dan tanda dari kelainan hiperandrogen serta anovulasi sehingga terbentuk polikistik pada ovarium. Pankreas merupakan organ yang terkena efek langsung dari kondisi PCOS. Resistensi insulin yang terjadi secara terus menerus akan menyebabkan efektivitas glukosa transporter mengalami penurunan dan glukosa yang tersirkulasi dalam darah meningkat sehingga pankreas melakukan kompensasi dengan hiperinsulinemia. Penelitian ini bertujuan untuk mengetahui pengaruh induksi testosteron propionat (TP) dan estradiol valerat (EV) pada hewan model PCOS terhadap kadar glukosa dan gambaran histopatologi pankreas tikus betina. Hewan model yang digunakan yaitu 18 ekor tikus Wistar (Rattus norvegicus) betina dengan berat 130-180 gram dan usia 5-6 bulan yang dibagi menjadi tiga kelompok perlakuan, yaitu kelompok kontrol negatif, kelompok perlakuan 1 yang diinduksi testosteron propionat 100 mg/kg BB selama 12 hari, dan kelompok perlakuan 2 yang diinduksi estradiol valerat 2 mg/kg BB selama 2 hari. Kadar glukosa diukur menggunakan glukometer dan dianalisis menggunakan ANOVA dan uji lanjutan BNT. Gambaran histopatologi pankreas dianalisis dengan metode skoring dan selanjutnya dianalisis dengan uji Kruskal Wallis dan jika terdapat perbedaan nyata dilanjutkan dengan uji Mann Whitney. Kadar glukosa darah pada tikus model PCOS menunjukkan adanya peningkatan. Kadar glukosa kelompok induksi EV mengalami peningkatan yang berbeda signifikan dengan kelompok kontrol dan induksi TP (p<0,05). Gambaran histopatologi pankreas tikus model PCOS menunjukkan kerusakan berupa vakuolisasi, nekrosis sel dan inflamasi pada kelompok perlakuan menunjukkan perbedaan signifikan (p<0,05) terhadap kelompok kontrol. Kesimpulan yang didapat adalah induksi estradiol valerat secara signifikan lebih dapat meningkatkan kadar glukosa darah dan berpengaruh terhadap adanya vakuolisasi dan nekrosis sel pada organ pankreas tikus model PCOS.
English Abstract
Polycystic Ovary Syndrome is a collection of symptoms and signs of hyperandrogenic disorders and anovulation resulting in the formation of polycystic ovaries. The pancreas is an organ that is directly affected by the PCOS condition. Insulin resistance that occurs continuously will cause the decrease of effectiveness glucose transporters and increase glucose circulating in the blood so that the pancreas compensates with hyperinsulinemia. This study aims to determine the effect of testosterone propionate (TP) and estradiol valerate (EV) induction in PCOS animal models on glucose levels and pancreatic histopathological features of female rats. The animal model used was 18 female Wistar rats (Rattus norvegicus) weighing 130-180 grams and aged 5-6 months which were divided into three treatment groups, namely the negative control group, treatment group 1 which was induced by testosterone propionate 100 mg/kg BW for 12 days, and treatment group 2 which was induced by estradiol valerate 2 mg/kg BW for 2 days. Glucose levels were measured using a glucometer and analyzed using ANOVA and BNT follow-up test. Pancreatic histopathological features were analyzed using the scoring method and then analyzed with the Kruskal-Wallis test and if there was a significant difference it was continued with the Mann-Whitney test. Blood glucose levels in the PCOS model rats showed an increase. Glucose levels in the EV-induced group experienced a significantly different increase from the control and TP-induced groups (p<0.05). Pancreatic histopathology of PCOS model rats showed damage in the form of vacuolization, cell necrosis and inflammation in the treatment group which showed significant differences (p<0.05) compared to the control group. The conclusion obtained was that induction of estradiol valerate significantly increased blood glucose levels and had an effect on the presence of vacuolization and cell necrosis in the pancreatic organ of the PCOS rat model
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | 052313 |
| Uncontrolled Keywords: | PCOS, Testosteron Propionat, Estradiol Valerat, Glukosa, Pankreas-PCOS, Testosterone Propionate, Estradiol Valerate, Glucose, Pancreas. |
| Divisions: | Fakultas Kedokteran Hewan > Kedokteran Hewan |
| Depositing User: | Unnamed user with email prayoga |
| Date Deposited: | 11 Jan 2024 03:26 |
| Last Modified: | 11 Jan 2024 03:26 |
| URI: | http://repository.ub.ac.id/id/eprint/208093 |
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