Muhammad Andhika, Andrianto and Irnia Nurika,, STP, MP, Ph.D and Nimas Mayang Sabrina S., STP, M.Sc, MP, Ph.D (2023) Pengaruh Penambahan Induser CuSO 4 dan Suhu Inkubasi Terhadap Degradasi Lignin Menggunakan Bakteri Lysinibacillus spaericus Pada Pretreatment Tandan Kosong Kelapa Sawit (TKKS). Sarjana thesis, Universitas Brawijaya.
Abstract
Tandan Kosong Kelapa Sawit (TKKS) merupakan biomassa lignoselulosa yang dihasilkan oleh produksi kelapa sawit sebagai salah satu limbah. TKKS memiliki potensi yang dapat dikonversi menjadi bioproduk dengan nilai ekonomis tinggi apabila kandungan lignoselulosa dapat didegradasi secara maksimal. Kandungan lignoselulosa terdiri dari selulosa (36-43%), hemiselulosa (15-25%), dan lignin (22-34%). Ketiga polimer tersebut memiliki ikatan kovalen, ester, dan ikatan hidrogen. Kandungan lignoselulosa ini juga menjadikan TKKS sebagai biomassa dengan potensi tinggi untuk dimanfaatkan menjadi bioproduk dengan nilai ekonomis tinggi yang dapat menggantikan bahan-bahan tidak terbarukan. Pretreatment biologis dengan mikroorganisme Lysinibacillus spaericus merupakan salah satu cara untuk merombak struktur lignoselulosa TKKS melalui produksi enzim ligninolitik yang dapat memecah dan memutus ikatan yang terjadi antara ketiga polimer. Diduga dengan adanya penambahan induser CuSO 4 serta variasi suhu inkubasi dapat meningkatkan aktivitas enzim ligninolitik serta kemampuan bakteri dalam mendegradasi lignoselulosa TKKS pada proses pretreatment. Penelitian bertujuan untuk mengevaluasi pengaruh penambahan induser CuSO 4 dan suhu inkubasi terhadap kemampuan bakteri Lysinibacillus spaericus dalam mendegradasi biomassa lignoselulosa TKKS pada proses pretreatment. Pada penelitian ini, pretreatment dilakukan dengan menggunakan bakteri Lysinibacillus spaericus sebagai mikroorganisme pendegradasi lignin terhadap biomassa lignoselulosa TKKS. Pengujian yang dilakukan pada penelitian meliputi uji Total Soluble Phenol (TSP), uji Total Gula Reduksi (TGR), uji pH, pengukuran weight loss, uji kadar air, dan uji kadar lignoselulosa. Rancangan penelitian yang digunakan adalah Rancangan Acak Kelompok (RAK) menggunakan 2 faktor. Faktor konsentrasi induser CuSO 4 (A) dengan tiga level konsentrasi yaitu 0,2 mM; 0,5 mM; dan 0,8 mM. Faktor variasi suhu inkubasi (B) dengan tiga level suhu 25 0 C, 30 0 C, dan 35 0 C. Terdapat 9 kombinasi sampel berbeda antara lain A1B1, A1B2, A1B3, A2B1, A2B2, A2B3, A3B1, A3B2, A3B3. Setiap perlakuan dilakukan pengulangan sebanyak 2 (dua) kali. Hasil dari pengujian akan dianalisis menggunakan Two-Way ANOVA dan uji DMRT. Penambahan induser CuSO 4 0,2; 0,5; dan 0,8 mM tidak memberikan pengaruh peningkatan aktivitas degradasi dibandingkan bakteri tanpa penambahan induser. Perlakuan suhu terbaik terhadap aktivitas degradasi lignoselulosa didapatkan pada suhu 30 0 C. Secara rata-rata, perlakuan bakteri tanpa penambahan induser di suhu 30 C memiliki nilai parameter tertinggi yaitu nilai TGR 9,492 mg/g, nilai TSP 0,0327 mg/g, persentase penurunan lignin sebesar 17,942%, persentase penurunan selulosa sebesar 6,297%, persentase peningkatan hemiselulosa sebesar 34,041%, dan pH terendah dengan nilai 6,85. Nilai persentase susut berat tertinggi didapatkan pada perlakuan penambahan CuSO 4 0,5 mM pada suhu 35 0 C.
English Abstract
Oil Palm Empty Fruit Bunches (OPEFB) is a lignocellulosic biomass produced by palm oil production as one of waste. OPEFB has the potential to be converted into bioproducts with high economic value if the lignocellulosic content can be degraded optimally. The lignocellulosic content in OPEFB consists of cellulose (36-43%), hemicellulose (15-25%), and lignin (22-34%). The three polymers have a covalent bond, ester bond, and hydrogen bond. Biological pretreatment with the microorganism Lysinibacillus spaericus is one way to breakdown the lignocellulosic structure of EFB through the production of ligninolytic enzymes that can breakdown and break the bonds that occur between the three polymers. It is suspected by the addition of CuSO 4 inducer and variations in incubation temperature can increase the activity of ligninolytic enzymes and the ability of bacteria to degrade lignocellulosic OPEFB in the pretreatment process. This study aims to evaluate the effect of the addition of CuSO 4 inducer and incubation temperature towards the ability of Lysinibacillus spaericus bacteria to degrade lignocellulosic OPEFB biomass in the pretreatment process. In this study, pretreatment was carried out using Lysinibacillus spaericus bacterium as a lignin-degrading microorganism for OPEFB lignocellulosic biomass. Tests carried out in this study included the Total Soluble Phenol (TSP) test, total sugar test, pH test, weight loss measurement, moisture content test, and lignocellulose content test. The research design used is Randomized Block Design (RBD) using 2 factors. CuSO 4 inducer concentration (A) with four concentration levels of 0,2 mM; 0,5 mM; and 0,8 mM. Variations in incubation temperature (B) with three temperature levels of 25 viii 0 C, 30 0 C, and 35 C. There are 9 different sample combinations, namely A1B1, A1B2, A1B3, A2B1, A2B2, A2B3, A3B1, A3B2, A3B3. Each treatment was repeated 2 (two) times and the results of the tests were analyzed using Two-Way ANOVA and DMRT test. The addition of CuSO4 inducer 0,2; 0,5; and 0,8 mM did not have the effect of increasing degradation activity compared to bacteria without the addition of inducer. The best temperature treatment for lignocellulosic degradation activity was obtained at 30 C. On average, the bacterial treatment without inducer addition at 30 0 C had the highest parameter values, namely TGR value of 9,492 mg/g, TSP value of 0,0327 mg/g, percentage of lignin reduction of 17,942%, percentage of cellulose reduction of 6,297%, percentage of increase in hemicellulose of 34,041%, and the lowest pH with a value of 6.85. The highest percentage value for weight loss was obtained in the treatment with the addition of 0,5 mM CuSO4 at 35 0 C.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | 052310 |
| Uncontrolled Keywords: | Lysinibacillus spaericus, Peningkatan Aktivitas Degradasi, Pretreatment, TKKS Degradation Activity, Lysinibacillus sphaericus, OPEFB, Pretreatment |
| Divisions: | Fakultas Teknologi Pertanian > Teknologi Industri Pertanian |
| Depositing User: | Unnamed user with username verry |
| Date Deposited: | 10 Jan 2024 08:35 |
| Last Modified: | 10 Jan 2024 08:35 |
| URI: | http://repository.ub.ac.id/id/eprint/207647 |
![[thumbnail of Dalam Masa Embargo]](http://repository.ub.ac.id/style/images/fileicons/text.png) |
Text (Dalam Masa Embargo)
Muhammad Andhika Andrianto.pdf Restricted to Registered users only until 31 December 2025. Download (3MB) |
Actions (login required)
 |
View Item |