Syasa, Anindita Mila and Dr. Ir Mintarto Martosudiro,, MS and Fery Abdul Choliq,, SP., MP., M.Sc (2023) Uji Efektivitas Ekstrak Daun Sirih (Piper Betle L) dalam Menghambat Pertumbuhan Jamur Alternaria sp. Penyebab Penyakit Bercak Coklat pada Tanaman Tomat (Lycopersicum esculentum Mill.). Sarjana thesis, Universitas Brawijaya.
Abstract
Salah satu jenis OPT (organisme pengganggu tanaman) penting yang sering menyerang tomat ialah penyakit bercak coklat yang disebabkan oleh jamur Alternaria sp. Kerusakan yang cukup tinggi karena serangan patogen ini dapat mengakibatkan pertumbuhan tanaman terhambat dan buah yang dihasilkan kecil dan busuk. Banyak dampak negatif dari penggunaan pestisida sintesis sebagai upaya penanganan penyakit ini, sehingga perlu adanya alternatif lain. Salah satunya ialah dengan penggunaan pestisida nabati antifungi atau fungisida. Penelitian ini bertujuan untuk mengetahui efektivitas ekstrak daun sirih dalam mengendalikan penyakit bercak coklat pada tanaman tomat. Penelitian ini akan dilaksanakan di Laboratorium Toksikologi Pestisida dan Laboratorium Penyakit Tumbuhan Jurusan Hama dan Penyakit Tumbuhan (HPT), Fakultas Pertanian (FP) Universitas Brawijaya (UB), pada bulan September 2022 hingga Maret 2023. Penelitian ini menggunakan rancangan acak lengkap (RAL) dengan satu faktor perlakuan. Pengujian dilakukan dengan 6 variasi konsentrasi dan 5 kali ulangan. Perlakuan yang digunakan antara lain kontrol, penambahan ekstrak daun sirih 2.000 ppm, penambahan ekstrak daun sirih 4.000 ppm, penambahan ekstrak daun sirih 6.000 ppm, penambahan ekstrak daun sirih 8.000 ppm, dan penambahan ekstrak daun sirih 10.000 ppm. Parameter yang diamati untuk uji in vitro yaitu penghambatan pertumbuhan koloni, presentase daya hambat koloni dan berat kering miselium. Parameter yang diamati untuk pengujian in vivo ialah diameter bercak dan daya perkecambahan. Data yang diperoleh dari hasil pengamatan dianalisis secara statistik menggunakan analisis ragam dan jika berbeda nyata dilanjutkan dengan uji lanjut BNT (Beda Nyata Terkecil) dengan taraf kesalahan 5% untuk mengetahui beda nyata antar perlakuan. Hasil penelitian menunjukkan bahwa ekstrak daun sirih mampu menghambat pertumbuhan jamur Alternaria sp. secara in vitro pada konsentrasi 4.000 ppm, 6.000 ppm, 8.000 ppm , dan 10.000 ppm, secara in vivo pada konsentrasi 6.000 ppm, 8.000 ppm, dan 10.000 ppm.
English Abstract
One of the important types of disease that often attack tomatoes is brown spot disease caused by the fungus Alternaria sp. The damage is quite high due to the attack of this pathogen can result in stunted plant growth and the resulting fruit is small and rotten. There are many negative impacts from the use of synthetic pesticides as the efort to prevent this disease, other alternatives are needed. One of them is the use of anti-fungal vegetable pesticides or fungicides. This study aims to determine the effectiveness of betel leaf in controlling brown spot disease in tomato plants. This research was conducted at the Mycology and Toxicology Laboratory of the Plant Pests and Diseases Department, Faculty of Agriculture, Brawijaya University in September 2022 - March 2023. This study uses a completely randomized design (CRD) with one treatment factor. The test used consisted of 6 variations of concentration and 5 replications. The treatments used include control, addition of betel leaves extract 2.000 ppm, addition of betel leaves extract 4.000 ppm, addition of betel leaves extract 6.000 ppm, addition of betel leaves extract 8.000 ppm, and addition of betel leaves extract 10.000 ppm. The parameters observed for the in vitro test were colony diameter, percentage of conidial inhibition and the weight of dry mycelium. The parameters observed for in vivo testing were measuring diameter and conidia germination. Data obtained from observations were statistically analyzed using analysis of variance and if significantly different were followed by further tests of LSD (Least Significant Difference) with an error rate of 5% to find out the real difference between treatments. The results showed that betel leaf extract was able to inhibit the growth of Alternaria sp. in vitro are 4.000 ppm, 6.000 ppm, 8.000 ppm, and 10.000 ppm, and in vivo treatment is 6.000 ppm, 8.000 ppm, and 10.000 ppm.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | 052304 |
| Divisions: | Fakultas Pertanian > Agroekoteknologi |
| Depositing User: | Unnamed user with username chikyta |
| Date Deposited: | 10 Jan 2024 08:07 |
| Last Modified: | 10 Jan 2024 08:07 |
| URI: | http://repository.ub.ac.id/id/eprint/207613 |
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