Pratiwi, Maharani Hari Nur and Dr. Uun Yanuhar,, S.Pi., M. Si (2023) Analisis Total Kelimpahan Bakteri Pada Kawasan Sungai Porong. Sarjana thesis, Universitas Brawijaya.
Abstract
Sungai Porong merupakan salah satu sungai besar yang berada pada wilayah Kabupaten Sidoarjo, Jawa Timur. Kondisi Sungai Porong masuk ke dalam perairan yang mengalami penurunan kualitas air yang disebabkan oleh cemaran mikroplastik. Mikroplastik dapat ditemukan di permukaan air dan di dasar atau pada sedimen. Keberadaanya di dasar sedimen dipengaruhi oleh gaya gravitasi dari densitas plastik lebih tinggi dari densitas air. Beberapa penelitian membuktikan bahwa terdapat beberapa bakteri yang memiliki kemampuan mendegradasi polimer mikroplastik, diantaranya Azotobacter, Bacillus megaterium, Pseudomonas sp., Ralstonia eutropha, Halomonas sp, dsb. Oleh karena itu perlu dilakukan penelitian mengenai bakteri baik itu jenis maupun kelimpahnnya, mikroplastik, serta kualitas air. Tujuan penelitian ini adalah untuk mengetahui kandungan mikroplastik serta bakteri yang terdapat pada Sungai Porong, bakteri tersebut dianalisis apakah dapat mendegradasi mikroplastik yang terkandung dalam air dan sedimen. Selain itu juga dilakukan analisis kualitas air sebagai parameter pendukung, yaitu parameter fisika (suhu dan TDS) dan parameter kimia (DO, CO2, TOM, dan pH). Dimana data diambil sebanyak lima kali ulangan. Penelitian ini dilaksanakan pada bulan Desember 2022 – Februari 2023. Metode pengambilan data pada penelitian ini dilakukan dengan 2 cara, yakni secara kuantitatif dan kualitatif. Data kuantitatif didapatkan dari data lapang dan laboratorium yang dilakukan oleh peneliti, sedangkan data kualitatif diperolah dari observasi, wawancara, partisipasi aktif, dokumentasi dan dari studi literatur yang terverifikasi dan terpercaya. Kesimpulan hasil penelitian berdasarkan data yang diperoleh pada pengukuran parameter kualitas air didapatkan nilai suhu berkisar 19,2–290C, kadar TDS berkisar 88,3–652,1 mg/L, nilai pH berkisar 6,7–7,2, konsentrasi DO berkisar 4,5–7,3 mg/L, konsentrasi CO2 berkisar 10,6–20,3 mg/L, dan kadar TOM berkisar 5,83–12,82 mg/L. Rata-rata kelimpahan mikroplastik pada air berkisar 3867–11200 partikel/m3. Rata-rata kelimpahan mikroplastik pada sedimen berkisar 578–1600 partikel/Kg. Rata-rata total kelimpahan bakteri berkisar antara 1,8 x 103 – 1,4 x 106. Jenis bakteri yang ditemukan adalah sebanyak 12 jenis bakteri di antaranya adalah Pseudomonas pseudomallei, Pseudomonas putida, Pantoea agglomerans, Klebsiella oxitoka, Acinetobacter baumannii, Klebsiella ozaenae, Escherichia coli, Streptomyces spp, Cronobacter sakazakii, Yersenia enterolitica, Providencia stuartii, dan Acinetobacter iwoffii. Saran yang diberikan adalah perlu dilakukan penelitian lebih lanjut sehingga bisa didapatkan data bakteri pendegradasi mikroplastik yang lebih detail serta perlu dilakukan pelestarian lingkungan sungai sehingga tingkat pencemaran Sungai Porong bisa menurun.
English Abstract
The Porong River is one of the major rivers in the Sidoarjo Regency, East Java. The condition of the Porong River enters the waters that have decreased water quality caused by microplastic contamination. Microplastics can be found on the surface of the water and on the bottom or in sediments. Its existence at the bottom of the sediment is influenced by the gravitational force of the plastic density being higher than the density of water. Several studies have proven that there are several bacteria that have the ability to degrade microplastic polymers, including Azotobacter, Bacillus megaterium, Pseudomonas sp., Ralstonia eutropha, Halomonas sp, etc. Therefore it is necessary to conduct research on bacteria, both types and abundance, microplastics, and water quality. The purpose of this study was to determine the content of microplastics and bacteria found in the Porong River, the bacteria were analyzed whether they could degrade the microplastics contained in water and sediment. In addition, water quality analysis was also carried out as supporting parameters, namely physical parameters (temperature and TDS) and chemical parameters (DO, CO2, TOM, and pH). Where the data was taken five times. This research was conducted in December 2022 – February 2023. The data collection method in this study was carried out in 2 ways, namely quantitatively and qualitatively. Quantitative data were obtained from field and laboratory data conducted by researchers, while qualitative data were obtained from observation, interviews, active participation, documentation and from verified and reliable literature studies. The conclusion of the research results based on the data obtained on the measurement of water quality parameters obtained temperature values ranging from 19.2–290C, TDS levels ranging from 88.3–652.1 mg/L, pH values ranging from 6.7–7.2, DO concentrations ranging 4.5–7.3 mg/L, CO2 concentrations ranged from 10.6–20.3 mg/L, and TOM levels ranged from 5.83–12.82 mg/L. The average abundance of microplastics in water ranges from 3867–11200 particles/m3. The average abundance of microplastics in sediment ranges from 578–1600 particles/Kg. The average total abundance of bacteria ranged from 1.8 x 103 – 1.4 x 106. The types of bacteria found were as many as 12 types of bacteria including Pseudomonas pseudomallei, Pseudomonas putida, Pantoea agglomerans, Klebsiella oxitoka, Acinetobacter baumannii, Klebsiella ozaenae, Escherichia coli, Streptomyces spp, Cronobacter sakazakii, Yersenia enterolitica, Providencia stuartii, and Acinetobacter iwoffii. The advice given is that it is necessary to carry out further research so that more detailed data on microplastic degrading bacteria can be obtained and it is necessary to preserve the river environment so that the pollution level of the Porong River can be reduced.ozaenae, Escherichia coli, Streptomyces spp, Cronobacter sakazakii, Yersenia enterolitica, Providencia stuartii, and Acinetobacter iwoffii. The advice given is that it is necessary to carry out further research so that more detailed data on microplastic degrading bacteria can be obtained and it is necessary to preserve the river environment so that the pollution level of the Porong River can be reduced.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | 0523080514 |
| Subjects: | 300 Social sciences > 333 Economics of land and energy > 333.9 Other natural resources > 333.95 Biological resources > 333.956 Fishes |
| Divisions: | Fakultas Perikanan dan Ilmu Kelautan > Manajemen Sumberdaya Perairan |
| Depositing User: | soegeng sugeng |
| Date Deposited: | 14 Dec 2023 07:27 |
| Last Modified: | 14 Dec 2023 07:27 |
| URI: | http://repository.ub.ac.id/id/eprint/205247 |
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