Efek Pemberian in-silico epitope based SARS-CoV-2 virus dengan adjuvant ISCOM secara per-oral terhadap peningkatan jumlah sel NK dan IgG Serum pada Mencit.

Sukmawati, Irma Nur and Prof. Dr. dr. Sumarno, DMM, SpMK (K) and dr. Dewi Santosaningsih, Sp. MK., M. Kes., PhD (2022) Efek Pemberian in-silico epitope based SARS-CoV-2 virus dengan adjuvant ISCOM secara per-oral terhadap peningkatan jumlah sel NK dan IgG Serum pada Mencit. Magister thesis, Universitas Brawijaya.

Abstract

Latar belakang.Vaksin adalah salah satu solusi terbaik untuk menanggulangi pandemi COVID-19. Vaksin epitope dapat dicari secara in silico. Pemilihan in silico epitope based SARS-CoV-2 yang digunakan sebagai kandidat vaksin harus dapat memicu respon imun, seperti protein dari spike (S), envelope (E), membrane (M) pada SARS-CoV-2. Tujuan penelitian. Untuk mengetahui potensi in silico epitope based SARS-CoV- 2 dari S, EM, dan SEM yang antigenik, non toksis dan non alergik. Serta mengevaluasi respon imun dengan pengukuran jumlah sel NK di limpa dan kadar IgG serum pada mencit. Metode. Penelitian ini dilakukan dalam 2 tahap, yaitu studi eksploratif in silico dan studi eksperimental. Pada study eksploratif yaitu pemilihan kandidat vaksin yang immunogenik, non toksik, non alergik, tes molecular docking dan konjugasi epitope dengan adjuvant berupa ISCOM yang diamati dengan Mikroskop TEM. Pada tahap eksperimental terdiri dari dua puluh lima ekor mencit (n=25) dibagi menjadi 5 kelompok. Kelompok pertama sebagai kontrol, dan kelompok kedua hanya diberikan ISCOM. Kelompok yang tersisa masing-masing diberikan epitope S, EM, dan SEM yang sudah dikonjugasi dengan ISCOM dan semua diberikan secara per-oral. Pada 5 kelompok dilakukan penghitungan sel NK dengan alat flowsitometer, sedangkan kadar IgG dengan pemeriksaan Elisa. Hasil penelitian.Uji in silico didapatkan tiga epitope dari S (FLVLLPLVSSQCVN), E (VNSVLLFLAFVVFLLVTLASS) dan M (LYIIKLIFLWLLWPVTLACFVLAAVY) yang antigenik, non toksik, dan non alergik. Pemberian in silico epitope based SARS-CoV-2 dapat meningkatkan jumlah sel NK terutama pada pemberian epitope S. Sedangkan kadar IgG serum tertinggi pada pemberian kombinasi epitope SEM. Kesimpulan. Pemberian in silico epitope based SARS-CoV-2 dapat meningkatkan jumlah sel NK di limpa dan Kadar IgG serum pada mencit

English Abstract

Background. Vaccines are one of the best solutions to deal with the COVID-19 pandemic. Epitope vaccines can be searched in silico. The selection of in silico epitope based SARS-CoV-2 which is used as a vaccine candidate must be able to trigger an immune response, such as proteins from the spike (S), envelope (E), membrane (M) in SARS-CoV-2. Research purposes. To determine the potential for in silico epitope based SARS- CoV-2 from S, EM, and SEM which is antigenic, non-toxic and non-allergenic. We evaluate the immune response by measuring the number of NK cells in the spleen and serum IgG levels in mice. Method. This research was conducted in 2 stages, in silico exploratory study and an experimental study. In an exploratory study, namely the selection of antigenic, non-toxic, non-allergic vaccine candidates, molecular docking tests and epitope conjugation with an adjuvant in the form of ISCOM observed with a TEM microscope. In the experimental stage, twenty-five mice (n=25) were divided into 5 groups. The first group was the control, and the second group was only given ISCOM. The remaining groups were each given the S, EM, and SEM epitope which had been conjugated with ISCOM and all were given orally. In 5 groups, NK cells were counted using a flow cytometer, while IgG levels were calculated using Elisa. The results. In silico test showed that three epitope S (FLVLLPLVSSQCVN), E (VNSVLLFLAFVVFLLVTLASS) and M (LYIIKLIFLWLLWPVTLACFVLAAVY) were antigenic, non-toxic, and non-allergic. Administration of in silico epitope based SARS-CoV-2 can increase the number of NK cells, especially when given the S epitope. Meanwhile, the highest serum IgG level was given with the combination of SEM epitope. Conclusion. Administration of in silico epitope based SARS-CoV-2 can increase the number of NK cells in the spleen and serum IgG levels in mice.

Item Type: Thesis (Magister)
Identification Number: 0423060062
Uncontrolled Keywords: in silico, epitope, SARS-CoV-2, Sel NK, IgG Serum
Subjects: 600 Technology (Applied sciences) > 616 Diseases > 616.9 Other disease > 616.904 Special topics of communicable diseases > 616.904 1 Medical microbiology / Drug resistance in microorganisms
Divisions: Profesi Kedokteran > Spesialis Patologi Klinik, Fakultas Kedokteran
Depositing User: Endang Susworini
Date Deposited: 05 Dec 2023 06:21
Last Modified: 05 Dec 2023 06:21
URI: http://repository.ub.ac.id/id/eprint/204751
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