Rizki, Siti Khofifah Budi and Dr. Ating Yuniarti,, S.Pi., M.Aqua and Nasrullah Bai Arifin,, S.Pi., M.Sc. (2023) Pengaruh Salinitas Terhadap Pertumbuhan, Biomassa, Kandungan Nutrisi, dan Karotenoid Aurantiochytrium sp. Sarjana thesis, Universitas Brawijaya.
Abstract
Aurantiochytrium sp. merupakan mikroalga laut yang bersifat heterotrof dan umumnya diisolasi dari berbagai sampel seperti air laut, sedimen, dan guguran daun dari kawasan mangrove. Aurantiochytrium sp. sebagai biflagellata osmoheterotrophic laut diketahui memiliki kandungan biomassa lipid yang tinggi. Karena mereka dapat mengakumulasi lebih dari 20–25% total asam lemak (TFA) dalam biomassa mereka. Selain itu mereka dapat menjadi sumber karotenoid, seperti ω3-PUFAs (DHA, EPA, dan DPA), protein, karbohidrat, dan squalene yang menjanjikan. Produksi biomolekul heterotrofik pada Aurantiochytrium sp., seperti ω3-PUFAs (DHA, EPA, dan DPA), protein, karbohidrat, dan squalene telah dinilai secara intraseluler dalam biomassa dan secara ekstraseluler dalam supernatan. Melihat kandungan nutrisi Aurantiochytrium sp. yang sangat potensial, optimalisasi proses pertumbuhan dalam kulturnya sangat penting untuk produksi kandungan nutrisinya. Strategi optimalisasi kultur yang dapat dilakukan yaitu memperhatikan beberapa faktor seperti salinitas, suhu, pH, pemilihan strain, tingkat oksigen terlarut, sumber karbon, sumber nitrogen, dan konsentrasi strain yang di kultur. Semua faktor ini mempengaruhi pertumbuhan, biomassa, dan produktivitas lipid dari Aurantiochytrium sp. Metode yang digunakan dalam penelitian ini yaitu metode eksperimen menggunakan Rancangan Acak Lengkap (RAL) dengan 4 perlakuan dan 3 ulangan. Perlakuan salinitas mediayang digunakan adalah 7 ppt, 16 ppt, 25 ppt, dan 32 ppt. Parameter utama yang diuji adalah pertumbuhan, biomassa, lipid, karbohidrat, protein dan karotenoid. Parameter penunjang yang diukur dalam penelitian adalah pH, suhu, salinitas, dan serapan glukosa. Hasil penelitian ini menunjukkan bahwa salinitas yang berbeda memiliki pengaruh nyata terhadap pertumbuhan, biomassa, kandungan nutrisi, dan karotenoid Aurantiochytrium sp.. Salinitas awal media 7 ppt memiliki hasil yang terbaik terhadap pertumbuhan dengan nilai doubling time 4,768 jam serta laju pertumbuhan spesifik sebesar 0,146 jam-1, biomassa sebesar 1,437 g/L, lipid sebesar 46,20%, protein 1,36 μg/gr, karbohidrat sebesar 33,97%, dan karotenoid sebesar 0,0846 μg/gr, . Hasil pengukuran parameter penunjang menunjukkan pH berkisar antara 6 hingga 7, suhu berkisar antara 28-30oC, dan salinitas berkisar antara 15-40 ppt. Berdasarkan penelitian ini, dapat disimpulkan bahwa pemberian salinitas awal media yang berbeda memberikan pengaruh berbeda nyata terhadap pertumbuhan, biomassa, kandungan nutrisi, dan karotenoid Aurantiochytrium sp. Penelitian selanjutnya disarankan menggunakan salinitas berbeda dalam penelitian Aurantiochytrium sp. dengan mengevaluasi parameter lainnya seperti squalene, pigmen, EPA, dan DHA serta nutrisi lainnya.
English Abstract
Aurantiochytrium sp. is a marine microalgae that is heterotrophic and is generally isolated from various samples such as seawater, sediments, and leaf fall from mangrove areas. Aurantiochytrium sp. as marine osmoheterotrophic biflagellates are known to have high lipid biomass content. Because they can accumulate more than 20–25% total fatty acids (TFA) in their biomass. In addition they can be a promising source of carotenoids, such as ω3-PUFAs (DHA, EPA, and DPA), protein, carbohydrates, and squalene. The production of heterotrophic biomolecules in Aurantiochytrium sp., such as ω3-PUFAs (DHA, EPA, and DPA), proteins, carbohydrates, and squalene has been assessed intracellularly in biomass and extracellularly in supernatants. Looking at the nutritional content of Aurantiochytrium sp. potential, optimizing the growth process in the culture is essential for the production of its nutritional content. Culture optimization strategies that can be carried out are paying attention to several factors such as salinity, temperature, pH, selection of strains, dissolved oxygen levels, carbon sources, nitrogen sources, and strain concentrations in culture. All of these factors affect the growth, biomass, and lipid productivity of Aurantiochytrium sp. The method used in this study was the experimental method using a completely randomized design (CRD) with 4 treatments and 3 replications. The media salinity treatments used were 7 ppt, 16 ppt, 25 ppt and 32 ppt. The main parameters tested were growth, biomass, lipids, carbohydrates, proteins and carotenoids. Supporting parameters measured in the study were pH, temperature, salinity, and glucose uptake. The results of this study indicate that different salinities have a significant effect on growth, biomass, nutrient content, and carotenoid Aurantiochytrium sp.. Initial media salinity of 7 ppt had the best results on doubling time of 4,768 h-1 and a specific growth rate of 0,146 hour-1, biomass of 1,437 g/L, lipids of 46,20%, protein 1,36 μg/gr, carbohydrates of 33,97%, and carotenoids of 0.0846 μg/gr, . The results of the supporting parameter measurements showed that the pH ranged from 6 to 7, the temperature ranged from 28-30oC, and the salinity ranged from 15-40 ppt. Based on this study, it can be concluded that the initial salinity administration of different media had significantly different effects on growth, biomass, nutrient content, and carotenoids of Aurantiochytrium sp. Further studies are suggested to use different salinities in the study of Aurantiochytrium sp. by evaluating other parameters such as squalene, pigments, EPA, and DHA as well as other nutrients.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | 0523080230 |
| Subjects: | 600 Technology (Applied sciences) > 639 Hunting, fishing & conservation > 639.3 Culture of cold-blooded vertebrates |
| Divisions: | Fakultas Perikanan dan Ilmu Kelautan > Budidaya Perairan |
| Depositing User: | soegeng sugeng |
| Date Deposited: | 19 Sep 2023 02:53 |
| Last Modified: | 19 Sep 2023 02:53 |
| URI: | http://repository.ub.ac.id/id/eprint/203031 |
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