Nusantari L.K.A., Raden Ajeng Safarina and Prof. Dr. Ir. Arief Prajitno,, M.S. and Ir. Heny Suprastyani,, M.S. (2022) Uji Daya Hambat Ekstrak Kasar Daun Keji Beling (Strobilanthes crispus) Terhadap Bakteri Vibrio parahaemolyticus Secara In Vitro. Sarjana thesis, Universitas Brawijaya.
Abstract
Udang vaname (Litopenaeus vannamei) merupakan spesies budidaya dan memiliki nilai ekonomis yang tinggi di dunia. Budidaya udang vaname berkembang pesat menggantikan budidaya udang windu. Penyakit ikan merupakan suatu perubahan kondisi fisik, morfologi serta fungsi dari keadaan normal menjadi tidak normal. Vibrio parahaemolyticus merupakan salah satu bakteri penyebab penyakit di lingkungan budidaya udang. Penyakit yang disebabkan oleh bakteri Vibrio sp. disebut dengan penyakit vibriosis. Penyakit vibriosis menyebabkan kematian masal pada budidaya udang mencapai 80 – 85% dari total populasi. Salah satu tanaman yang berpotensi menjadi tanaman obat yaitu daun keji beling (Strobilanthes crispus). Daun keji beling (S. crispus) telah banyak dimanfaatkan oleh masyarakat untuk pengobatan tradisional. Kandungan senyawa kimia dalam daun keji beling (S. crispus) memiliki banyak manfaat dan kandungan senyawa fitokimia antara lain kalium, natrium, kalsium, asam silikat, asam salisilat, kristal kalsium karbonat, alkaloid, saponin, flavonoid, polilenoid dan tanin. Penelitian ini dilaksanakan mulai tanggal 24 Januari 2022 – 19 Februari 2022 Di Laboratorium Sentral Ilmu Hayati (LSIH), Universitas Brawijaya, Malang. Tujuan dari penelitian ini adalah untuk mengetahui daya hambat ekstrak kasar daun keji beling (S. crispus) terhadap bakteri V. parahaemolyticus secara in vitro. Metode yang digunakan adalah eksperimental. Rancangan penelitian yang digunakan yaitu Rancangan Acak Lengkap (RAL) menggunakan 5 dosis dengan 3 kali ulangan. Dosis perlakuan yang digunakan yaitu A (25 ppm), B (50 ppm), C (75 ppm), D (100 ppm), dan E (125 ppm). Hasil penelitian menunjukkan dosis ekstrak kasar yang memberikan hasil tertinggi pada diameter zona hambat terhadap bakteri V. parahaemolyticus adalah perlakuan E dengan dosis 125 ppm diperoleh rerata sebesar 8,93 + 0,78 mm. Dosis ektrak yang memiliki hasil terendah yaitu pada perlakuan A dengan dosis 25 ppm diperoleh rerata 7,53 + 0,50 mm. Hubungan antara dosis perlakuan dengan diameter zona hambat menunjukkan pola linier dengan persamaan y = 0,0029x + 8,1695 dan koefisien regresi sebesar 0,586. Kesimpulan hasil penelitian ini bahwa ekstrak kasar daun keji beling memiliki daya hambat terhadap bakteri V. parahaemolyticus secara in vitro dan memiliki sifat bakterisidal atau mampu membunuh bakteri. Diameter zona hambat yang didapatkan berkisar 7,53 – 8,93 mm.
English Abstract
Vannamei shrimp (Litopenaeus vannamei) is a cultivated species and has high economic value in the world. Vannamei shrimp cultivation is growing rapidly in tiger shrimp cultivation. Disease is a change in physical condition, morphology and function from normal to abnormal conditions. Vibrio parahaemolyticus is one of the bacteria that causes disease in shrimp culture. Diseases caused by the bacteria Vibrio sp. called vibriosis. Vibriosis disease causes mass mortality in shrimp farming reaching 80-85% of the total population. One of the plants that is used as a medicinal plant is the keji beling leaf (Strobilanthes crispus). The leaves of the keji beling (S. crispus) have been widely used by the community for traditional medicine. The content of chemical compounds in the leaves of keji beling (S. crispus) has many benefits and contains phytochemical compounds including potassium, sodium, calcium, silicic acid, salicylic acid, calcium carbonate crystals, alkaloids, saponins, flavonoids, polylenoid and tannins. This research was conducted from January 24, 2022 – February 19, 2022 at the Central Laboratory of Life Sciences (LSIH), Universitas Brawijaya, Malang. The purpose of this study was to determine the inhibition of crude extract of the leaves of Keji beling (S. crispus) against V. parahaemolyticus bacteria in vitro. The method used is experimental method. The research used was a Completely Randomized Design (CRD) using 5 doses with 3 replications. The treatment doses used were A (25 ppm), B (50 ppm), C (75 ppm), D (100 ppm), and E (125 ppm). The results showed that the dose of crude extract that gave the highest yield on the diameter of the inhibition zone against V. parahaemolyticus was treatment E with a dose of 125 ppm, the average was 8.93 + 0.78 mm. The extract dose that had the lowest yield was in treatment A with a dose of 25 ppm, the average was 7.53 + 0.50 mm. The relationship between the dose of S. crispus leaves extract and the diameter of the inhibition zone was resulted in a linear pattern with the equation y = 0,0029x + 8,1695 and coefficient regression was 0,586. The conclusion of this study was that the crude extract of the leaves of Keji beling had inhibitory power against V. parahaemolyticus bacteria in vitro and had bactericidal properties or was able to kill bacteria. The diameter of the inhibition zone obtained ranges from 7.53 to 8.93 mm.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | 0522080543 |
| Subjects: | 600 Technology (Applied sciences) > 639 Hunting, fishing & conservation > 639.3 Culture of cold-blooded vertebrates |
| Divisions: | Fakultas Perikanan dan Ilmu Kelautan > Budidaya Perairan |
| Depositing User: | soegeng sugeng |
| Date Deposited: | 15 Sep 2023 09:02 |
| Last Modified: | 15 Sep 2023 09:02 |
| URI: | http://repository.ub.ac.id/id/eprint/202977 |
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