Marwulan, - and Prof. Dr. Ir. Happy Nursyam,, M.S and Dr. Yuni Kilawati,, S.Pi., M.Si. (2023) Potensi Penggunaan Ekstrak Tanaman Lantana camara Linn. terhadap Profil Hematologi dan Histopatologi Insang dan Hati Ikan Mas (Cyprinus carpio) yang diinfeksi Bakteri Edwardsiella tarda. Magister thesis, Universitas Brawijaya.
Abstract
Penyakit yang sering menginfeksi ikan budidaya khususnya ikan mas (Cyprinus carpio) adalah penyakit bakterial yang berasal dari bakteri E. tarda. Penggunaan bahan alami menjadi alternatif pengobatan bakteri E. tarda karena penggunaan bahan kimia memiliki dampak negatif. Bahan alami yang digunakan sebagai antibakteri adalah daun L. camara Linn. dengan kandungan bahan aktif flavonoid, alkaloid, tannin, dan triterpenoid. Tujuan penelitian ini untuk menganalisis senyawa pada daun Lantana camara Linn. dan menganalisis dosis terbaik serta gambaran profil hematologi dan histopatologi ikan mas yang diinfeksi E. tarda. Penelitian ini dilaksanakan pada bulan Maret 2023 - Mei 2023 di Laboratorium Budidaya Ikan Divisi Penyakit dan Kesehatan Ikan, Fakultas Perikanan dan Ilmu Kelautan Universitas Brawijaya Malang. Penelitian ini menggunakan metode eksperimen dengan rancangan acak lengkap (RAL) dan terbagi menjadi dari 2 tahapan. Penelitian tahap pertama meliputi ekstraksi dan karakterisasi senyawa antibakteri pada L. camara Linn. (uji fitokimia, FTIR dan GC-MS) dilanjutkan dengan uji aktivitas antibakteri E. tarda meliputi uji cakram, uji MIC dan uji SEM. Tahap kedua, meliputi pengaplikasian ekstrak L. camara Linn. yang bersifat antibakteri pada ikan mas yang diinfeksi E. tarda pada skala laboratorium (uji patogenitas bakteri E. tarda, uji toksisitas ekstrak, pengamatan hematologi yang terdiri dari eritrosit, leukosit, differensial leukosit, hemoglobin, pengamatan histopatologi insang dan hati, pengamatan gejala klinis, kelulushidupan, dan kualitas air). Analisis data pada penelitian ini menggunakan aplikasi software Statistical Package for the Social Sciences (SPSS) 25.0 for Windows. Hasil penelitian tahap pertama, hasil fitokimia ekstrak L. camara Linn. mengandung flavonoid, alkaloid, tannin, dan triterpenoid. Hasil uji GC-MS didapatkan bahwa ekstrak Lantana camara Linn. memiliki komponen senyawa terbanyak yaitu 4H-1-Benzopyran-4-one, 5,7-dihydroxy-6-methoxy-2-(4-methoxyphenyl). Hasil uji MIC (Minimum Inhibitory Concentration) didapatkan nilai absorbansi terendah pada konsentrasi 100 mg/L dengan nilai 0,602 mendekati kontrol positif. Hasil cakram dengan dosis 50, 100, 150, 200, dan 250 mg/L. Hasil cakram menunjukkan dosis 200 mg/L memiliki zona hambat paling kuat dengan nilai 12,63 mm. Hasil penelitian tahap kedua, uji patogenitas (LD50) bakteri E. tarda didapatkan kepadatan sebesar 1,38 x 10^7 sel/ml dan uji toksisitas (LC50) ekstrak Lantana camara Linn pada konsentrasi 282,63 ppm berdasarkan hasil probit. Pemberian ekstrak L. camara Linn. terbukti memberikan pengaruh signifikan setiap perlakuan terhadap eritrosit, leukosit, differensial leukosit, dan hemoglobin serta histopatologi. Nilai dosis ekstrak L. camara Linn. yang paling optimal dan berpengaruh terhadap semua parameter yaitu pada dosis 200 mg/L.
English Abstract
The disease that often infects cultivated fish, especially carp (Cyprinus carpio) is a bacterial disease originating from E. tarda bacteria .The use of natural ingredients is an alternative treatment for E. tarda bacteria because the use of chemicals has a negative impact. Material naturally used as antibacterial is leaf L. Camara Linn. contains active ingredients of flavonoids, alkaloids, tannins, and triterpenoids. Objective study This For analyze compounds in the leaves of L. camara Linn. and analyze dose best as well as description profile hematology and histopathology of carp infected with E. tarda .This research was carried out in March 2023 - May 202 3 at the Fish Culture Laboratory, Division of Fish Disease and Health, Faculty Fisheries and Marine Sciences , University of Brawijaya Malang. This study used an experimental method with a completely randomized design (CRD) and was divided into 2 stages. Study stage First covers extraction and characterization compound antibacterial in L. Camara Linn. (phytochemical test, FTIR and GC-MS) followed by E. tarda antibacterial activity test including disc test, MIC test and SEM test. Stage second includes application extract L. Camara Linn. which has antibacterial properties in common carp infected with E. tarda on a laboratory scale (test E. tarda bacterial pathogenicity, extract toxicity test, hematological observations consisting of from erythrocytes, leukocytes, differential leukocytes, hemoglobin, observations histopathology gills and liver, observation of clinical symptoms, survival, and water quality). Data analysis on research This use software application Statistical Package for the Social Sciences (SPSS) 25.0 for Windows. Research results stage first, results phytochemicals extract L. camara Linn. contains flavonoids, alkaloids, tannins, and triterpenoids. The results of the GC-MS test showed that the Lantana camara Linn. has the most component compounds that is 4H-1-Benzopyran-4-one, 5,7-dihydroxy-6-methoxy-2-(4-methoxyphenyl). The MIC (Minimum Inhibitory Concentration) test results obtained the lowest absorbance value at a concentration of 100 mg/L with a value of 0.602 close to the positive control. Results of discs with doses of 50, 100, 150, 200, and 250 mg/L. The disc results showed that the dose of 200 mg/L had the strongest inhibition zone with a value of 12.63 mm. The results of the second phase of the study, the pathogenicity test (LD50) of E. tarda bacteria obtained a density of 1.38 x 10^7 cells/ml and the toxicity test (LC50) of L. camara Linn extract at a concentration of 282.63 ppm based on the probit results. Administration of extract L. Camara Linn. proven to deliver influence significant every treatment to erythrocytes, leukocytes, differential leukocytes , and hemoglobin as well as histopathology . Dosage value extract L. Camara Linn. the most optimal and influential all parameters that is at a dose of 200 mg/L
| Item Type: | Thesis (Magister) |
|---|---|
| Identification Number: | 0423080008 |
| Subjects: | 600 Technology (Applied sciences) > 639 Hunting, fishing & conservation > 639.3 Culture of cold-blooded vertebrates |
| Divisions: | S2/S3 > Magister Budidaya Perairan, Fakultas Perikanan dan Ilmu Kelautan |
| Depositing User: | soegeng sugeng |
| Date Deposited: | 08 Sep 2023 01:41 |
| Last Modified: | 08 Sep 2023 01:41 |
| URI: | http://repository.ub.ac.id/id/eprint/202852 |
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