Utomo, William Putra and drh. Fajar Shodiq Permata,, M.Biotech (2023) Perbandingan Pemberian Darah Allograft Dan Xenograft Untuk Induksi Hewan Model Autoimmune Hemolytic Anemia Pada Mencit Berdasarkan Jumlah Eritrosit Dan Histopatologi Ginjal. Sarjana thesis, Universitas Brawijaya.
Abstract
Autoimmune hemolytic anemia (AIHA) merupakan penyebab anemia yang paling umum ditemukan dengan tingkat mortalitas yang tinggi, terutama pada anjing. Namun belum ada standar resimen terapi yang tetap, sehingga pembuatan hewan model AIHA diharapkan dapat membantu penelitian selanjutnya untuk membantu lebih memahami patogenesa, terapi dan deteksi dini AIHA. Hewan coba yang digunakan adalah 18 ekor mencit (Mus musculus) jantan berumur 8-12 minggu dan berat badan 20-25 gram. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dan dibagi menjadi 3 kelompok, yaitu kelompok kontrol negatif tanpa perlakuan sebagai kontrol pembanding, kelompok P1 yaitu kelompok yang diberi darah allograft dari mencit dengan strain yang sama dengan hewan coba, dan kelompok P2 yang diberi darah xenograft dari kucing domestik. Masing-masing mencit diinjeksi darah allograft/xenograft secara intraperitoneal sebanyak 0,2 ml 5 hari per minggu selama 7 minggu. Parameter yang digunakan adalah jumlah eritrosit menggunakan Complete Blood Count dan histopatologi ginjal dengan pewarnaan hematoxyline-eosin. Jumlah eritrosit akan dianalisis dengan uji One Way ANOVA dan histopatologi ginjal akan dianalisis secara deskriptif kualitatif. Hasil penelitian menunjukkan bahwa Ginjal kelompok Allograft mengalami pembesaran dibandingkan Xenograft yang mengalami pengecilan secara makroskopis. Kelompok Allograft dan Kelompok Xenograft mengalami penurunan jumlah eritrosit yang signifikan terhadap kelompok perlakuan kontrol negatif. Gambaran histopatologi hepar kelompok Allograft menunjukkan adanya sel radang dan nekrosis di bagian medulla. Gambaran pada kelompok Xenograft menunjukkan edema pada gambaran histopatologis dan mengalami nekrosis pada bagian korteks dan banyak ditemukan sel radang
English Abstract
Autoimmune hemolytic anemia (AIHA) is the most common cause of anemia found with a high mortality rate, especially in dogs. However, there are no fixed therapeutic regiment standard, so the creation of AIHA animal models is expected to help further research to help better understand the pathogenesis, therapy and early detection of AIHA. The experimental animals used were 18 male mice (Mus musculus) aged 8-12 weeks and weighed 20-25 grams. This study used a Completely Randomized Design (CRD) and was divided into 3 groups, namely the negative control group without treatment as a comparison control, the P1 group was the allograft blood group of mice with the same strain as experimental animals, and the P2 group that was given xenograft blood from domestic cats. Each mice intraperitoneally injected allograft / xenograft blood as much as 0.2 ml 5 days per week for 7 weeks. The parameters used were the number of erythrocytes using Complete Blood Count and kidney histopathology with hematoxyline-eosin staining. The results showed that the Kidneys of the Allograft group experienced an experience compared to the Xenograft which experienced macroscopic reduction. The parameters of this study were the amount of erithrocytes and renal histopathology (HE staining). Erithrocyte levels will be analyzed by One Way ANOVA test and renal histopathology will be analyzed descriptively qualitatively. The Allograft group and the Xenograft group experienced a significant decrease in the negative control treatment. Histopathological picture of the liver in the Allograft group showed the presence of supporting cells and necrosis in the medulla. The picture in the Xenograft group showed edema on the histological picture and had necrosis in the cortex and many image cells were found
Other obstract
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| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | 0523130031 |
| Uncontrolled Keywords: | Autoimmune hemolytic anemia, hewan model, mencit, jumlah eritrosit, histopatologi ginjal, Autoimmune hemolytic anemia, animal model, mice, erythrocyte, kidney |
| Subjects: | 600 Technology (Applied sciences) > 636 Animal husbandry > 636.08 Specific topics in animal husbandry > 636.089 Veterinary medicine |
| Divisions: | Fakultas Kedokteran Hewan > Kedokteran Hewan |
| Depositing User: | Nur Cholis |
| Date Deposited: | 29 Aug 2023 01:56 |
| Last Modified: | 29 Aug 2023 01:56 |
| URI: | http://repository.ub.ac.id/id/eprint/202687 |
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