Rizqi, Ade Bestari Anggun and drh. Reza Yesica, M.Sc (2023) Efek Suplementasi Likopen Terhadap Perkembangan Embrio Dan Mortality Rate Serta Survival Rate Ikan Zebra (Danio Rerio ) Pada Proses In Vitro Culture Dengan Paparan Heat Stress. Sarjana thesis, Universitas Brawijaya.
Abstract
Stres panas dapat menyebabkan gangguan sinyal dan kerusakan sel sehingga menyebabkan kegagalan perkembangan sel hingga kematian sel. Kegagalan perkembangan embrio dan kematian embrio akan berdampak pada survival rate dan mortality rate embrio ikan zebra. Likopen berfungsi sebagai antioksidan terhadap radikal bebas sehingga memungkinkan organisme tetap hidup dalam kondisi stres. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh suplementasi likopen dengan tiga konsentrasi 0 μL, 2 μL dan 4 μL terhadap perkembangan embrio, tingkat mortalitas dan survival rate embrio ikan zebra di bawah suhu normal 28 oC (non heat stress) dan dengan paparan heat stress suhu 36 oC. Sebanyak 240 embrio ikan zebra berusia 5 hpf (hours post fertilization) dibagi ke dalam 3 kelompok perlakuan meliputi dosis pemberian 0 μL, 2 μL dan 4 μL likopen dengan 4 kali pengulangan. Data kuantitatif kemudian diolah menggunakan software SPSS dengan Analisa Ragam ANOVA. Likopen mampu melindungi embrio dari stres oksidatif selama masa perkembangan ditandai semua embrio kelompok perlakuan dengan suplementasi likopen berkembang dan menetas dalam waktu perkembangan normal. Hasil uji ANOVA menunjukkan bahwa suplementasi likopen berpengaruh nyata terhadap mortalitas (9,58±2,5%; P=0,001) dan survival rate (90,42±2,5%; P=0,005). Persentase mortalitas pada perlakuan HS 4 μL likopen (5±2,8%) lebih rendah dibandingkan mortalitas pada perlakuan kontrol NHS (7,5±4,7%) membuktikan bahwa likopen dapat memperbaiki mortalitas embrio seperti pada kelompok kontrol NHS. Persentase survival rate HS 4 μL (95±2,8%) lebih tinggi dibanding kontrol NHS (92,5±4,7%). Temuan ini menunjukkan bahwa likopen berpotensi sebagai antioksidan terhadap kematian embrio dan meningkatkan survival rate pada kondisi stres oksidatif akibat modulasi heat stress.
English Abstract
Heat stress could induce interference cell signal transduction and physiological dysfunction that may result in cell death. The disruption of embryonic development and embryonic death will have an impact on the survival rate and mortality rate of zebrafish embryos. Lycopene as a powerful antioxidant to attenuate free radicals can allows organisms to live under stress conditions. The aim of the study is to evaluate the effect of lycopene supplementation with three concentration 0 μL, 2 μL, and 4 μL on development stage, mortality rate, and survival rate zebrafish embryo under normal temperature 28 oC and heat stress induced at 36 oC. Total of 240 zebrafish embryos at 5 hpf of age were used and divided into 3 treatment groups including doses without lycopene, 2 μL lycopene, and dan 4 μL lycopene with 4 replicates. The quantitative data was then processed by statistical analysis using the SPSS application with ANOVA method. Lycopene has the ability to protect the embryos against oxidative stress during embryo development resulting all embryos with lycopene supplementation hatch and developed in normal period. The ANOVA result revealed that lycopene supplementation have a significance value of the mortality rate (9,58±2,5%; P=0,001) and survival rate (90,42±2,5%; P=0,005). The percentage of mortality rate was lower in HS 4 μL lycopene (5±2,8%) than NHS control (7,5±4,7%) prove that lycopene could decrease mortality rate similar to NHS control. The percentage of survival rate was higher in HS 4 μL lycopene (95±2,8%) than NHS control (92,5±4,7%). These findings indicate the potential of lycopene as a protective agent against embryonic mortality and increased the survival rate by heat stress modulation of oxidative stress
Other obstract
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| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | 0523130026 |
| Uncontrolled Keywords: | ikan zebra, heat stress, likopen, mortality rate, survival rate, Zebrafish, heat stress, lycopene, mortality rate, survival rate |
| Subjects: | 600 Technology (Applied sciences) > 636 Animal husbandry > 636.08 Specific topics in animal husbandry > 636.089 Veterinary medicine |
| Divisions: | Fakultas Kedokteran Hewan > Kedokteran Hewan |
| Depositing User: | Nur Cholis |
| Date Deposited: | 28 Aug 2023 03:23 |
| Last Modified: | 23 Oct 2023 02:42 |
| URI: | http://repository.ub.ac.id/id/eprint/202665 |
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