Alamsyah, Aldi Surya and Prof. Dr. Ir. Eddy Suprayitno,, MS (2023) Karakterisasi Peptida Hidrolisat Kolagen Kulit Ikan Gabus (Channa striata) sebagai Anti-Hipertensi. Sarjana thesis, Universitas Brawijaya.
Abstract
Hipertensi merupakan salah satu penyebab morbiditas terbesar didunia. Sistem RAAS (Renin-Angiotensin Aldosteron Secretion) adalah faktor utama dalam memelihara tekanan darah. Angiotensin I yang diubah menjadi Angiotensin II oleh enzim ACE (Angiotensin Converting Enzyme) bertanggung jawab dalam peningkatan tekanan darah. Obat antihipertensi yang beredar di pasaran bertindak sebagai ACE inhibitor masih memiliki efek samping. Oleh karena itu, dibutuhkan alternatif lain dalam menghambat enzim ACE. Peptida bioaktif dari hidrolisat kolagen merupakan salah satu solusi karena diketahui dapat berperan sebagai ACE inhibitor, sehingga penurunan tekanan darah dapat terjadi. Dibutuhkan sumber peptida hidrolisat kolagen yang potensial salah satunya yaitu Kulit Ikan Gabus (Channa striata) yang diketahui memiliki kandungan kolagen ± 5,96%. Oleh karena itu, dilakukan penelitian ini dengan tujuan mengetahui karakteristik peptida hidrolisat kolagen kulit ikan gabus (Channa striata) sebagai antihipertensi. Metode penelitian yang digunakan adalah deskriptif dengan dua tahap penelitian. Tahap pertama bertujuan untuk menghasilkan enzim protease dari daun biduri tua dan hidrolisat kolagen dari kulit ikan gabus. Tahap kedua bertujuan untuk mengetahui karakteristik peptida hidrolisat kolagen kulit ikan gabus dengan beberapa pengujian seperti, derajat hidrolisis, pH, kadar protein, asam amino, berat molekul, dan aktivitas antioksidan yang mendukung sifat antihipertensi hidrolisat kolagen kulit ikan gabus (Channa striata). Berdasarkan hasil proses pembuatan hidrolisat kolagen kulit ikan gabus (Channa striata) didapatkan rendemen sebesar 6%, diketahui pula derajat hidrolisis sebesar 18,79%, meskipun rendah namun tetap terjadi hidrolisis sehingga dapat memutus ikatan peptida kolagen menjadi fragmen yang lebih kecil. Sejalan dengan besarnya derajat hidrolisis, berat molekul dari peptida hidrolisat kolagen kulit ikan gabus didapatkan berat molekul terendah mulai 21,6 kDa sampai yang terbesar 238,6 kDa yang menandakan bahwa hidrolisis terjadi tidak merata. Didapatkan sekuen peptida Asp-Glu-Ser-Gly-His-Arg-Thr-Ala-Pro-Tyr-Val-Met-Cys-Ile-Leu-Phe-Lys, terdapat asam amino yang berpotensi sebagai ACE inhibitor yaitu asam amino aromatik dan hidrofobik (Tyr dan Phe), asam amino bermuatan positif (Arg, Lys, His), asam amino bermuatan negatif (Glu) dengan mekanisme berinteraksi dengan sisi aktif enzim ACE pada atom H, atom karboksil dan ion Zn secara berturut-turut.
English Abstract
Hypertension is one of the biggest causes of morbidity in the world. The RAAS (Renin-Angiotensin-Aldosterone Secretion) system is a major factor in maintaining blood pressure. Angiotensin I which is converted to Angiotensin II by the enzyme ACE (Angiotensin Converting Enzyme) is responsible for increasing blood pressure. Antihypertensive drugs on the market acting as ACE inhibitors still have side effects. Therefore, another alternative is needed to inhibit the ACE enzyme. Bioactive peptides from collagen hydrolyzate are one solution because they are known to act as ACE inhibitors, so that a decrease in blood pressure can occur. A potential source of collagen hydrolyzate peptides is needed, one of which is Snakehead Fish Skin (Channa striata) which is known to have a collagen content of ± 5.96%. Therefore, this research was conducted to know the characteristics of hydrolyzed collagen peptides from snakehead fish (Channa striata) skin as antihypertensives. The research method used is descriptive with two stages of research. The first stage aims to produce protease enzymes from old biduri leaves as hydrolyzers and hydrolyze snakehead fish skin collagen with old biduri leaf protease enzymes. The second stage aims to determine the characteristics of the hydrolyzed collagen peptides of snakehead fish skin collagen with several tests such as the degree of hydrolysis, pH, protein content, amino acids, molecular weight, and antioxidant activity which support the antihypertensive properties of hydrolyzed snakehead fish skin collagen (Channa striata). From the results of the process of making collagen hydrolyzate from snakehead fish (Channa striata) skin, a yield of 6% was obtained, it was also known that the degree of hydrolysis was 18.79%, although it was low, hydrolysis still occurred so that it could break the collagen peptide bonds into smaller fragments. In line with the degree of hydrolysis, the molecular weight of hydrolyzed snakehead collagen peptide obtained from the lowest molecular weight ranging from 21.6 kDa to the largest 238.6 kDa, which indicates that the hydrolysis occurs unevenly. The Asp-Glu-Ser-Gly-His-Arg-Thr-Ala-Pro-Tyr-Val-Met-Cys-Ile-Leu-Phe-Lys peptide sequences, there are amino acids that have potential as ACE inhibitors, namely aromatic amino acids and hydrophobic (Tyr and Phe), positively charged amino acids (Arg, Lys, His), negatively charged amino acids (Glu) with the mechanism of interacting with the active site of the ACE enzyme on H atoms, carboxyl atoms and Zn ions respectively
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | 0523080063 |
| Uncontrolled Keywords: | ACE, Antihipertensi, Hidrolisat, Ikan Gabus, Peptida,ACE, Antihypertension, Hydrolysate, Peptide, Snakehead Fish |
| Subjects: | 600 Technology (Applied sciences) > 639 Hunting, fishing & conservation > 639.2 Commercial fishing, whaling, sealing |
| Divisions: | Fakultas Perikanan dan Ilmu Kelautan > Teknologi Hasil Perikanan |
| Depositing User: | soegeng sugeng |
| Date Deposited: | 28 Aug 2023 02:13 |
| Last Modified: | 28 Aug 2023 02:13 |
| URI: | http://repository.ub.ac.id/id/eprint/202654 |
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