Rinady, Maydella Vista Putri and Dr. Ir. Yulia Nuraini, and Cahyo Prayogo, and Novi Arfarita, (2023) Potensi Kelimpahan Bakteri Selulolitik Pada Berbagai Jenis Sistem Agroforestri Di Kawasan Ub Foresti. Magister thesis, Universitas Brawijaya.
Abstract
Pengelolaan sistem agroforestri yang berbeda mengubah komposisi dan pertumbuhan tanaman. Hal ini akan mempengaruhi masukan bahan organik yang menjadi sumber makanan dan energi bagi mikroorganisme tanah. Organisme tanah itu sendiri memainkan peran penting dalam memperkuat proses biogeokimia tanah dan mengatur reaksi tanah. Proses ini memungkinkan unsur hara dilepaskan ke dalam tanah dan diserap oleh tanaman. Oleh sebab itu, penelitian ini dilakukan bertujuan untuk menambahkan informasi mengenai kelimpahan bakteri selulolitik, aktivitas isolat bakteri selulolitik yang ditemukan, serta hubungan perbedaan penggunaan lahan yang mempengaruhi kualitas serasah terhadap kelimpahan bakteri selulolitik yang ditemukan di berbagai penggunaan lahan di kawasan UB Forest. Penelitian telah dilakukan di UB Forest pada bulan Februari hingga Juli 2022. Analisis bakteri selulolitik dilaksanakan di Laboratorium Universitas Islam Malang. Analisis laboratorium dilaksanakan di Laboratorium Jurusan Tanah, Fakultas Pertanian, Universitas Brawijaya. Penelitian dilakukan menggunakan metode survey di berbagai penggunaan lahan di kawasan UB Forest yang dibedakan berdasarkan tutupan lahan. Rancangan yang digunakan dalam penelitian ini adalah rancangan acak kelompok (RAK). Titik plot pengamatan yaitu agroforestri pinus-kopi (PK), agroforestri pinus-pisang (PPs), agroforestri pinus-rempah (PR), agroforestri pinus-sayuran (PS/kontrol), kebun campuran (KC) dan lahan jeruk (LJ). Terdapat 6 titik plot pengamatan dengan ulangan sebanyak 4 kali di lokasi yang berbeda, sehingga total plot penelitian yaitu 24 plot (ukuran 20 m x 20 m). Variabel yang diukur dalam penelitian ini meliputi biologi tanah (total populasi bakteri, total populasi bakteri selulolitik dan aktivitas bakteri selulolitik), kimia tanah (pH, C organik, N total), bahan organik tanah (kandungan lignin, polifenol, berat basah dan kering serasah, kualitas serasah (C/N rasio) dan ketebalan serasah), vegetasi (diameter batang dan tutupan kanopi), dan iklim mikro (suhu tanah, suhu udara dan kelembaban). Analisis data menggunakan Analysis of Variance (ANOVA) pada taraf 5%, dilanjutkan dengan uji BNJ untuk menganalisis perbedaan antar perlakuan. Guna mengetahui hubungan dan pengaruh antar variabel menggunakan uji korelasi dan regresi. Hasil penelitian menunjukkan bahwa bahwa kandungan C dan N organik tertinggi diperoleh pada petak PK (agroforestri pinus umur 41 tahun dan kopi umur 11 tahun) yaitu sekitar 6,64% dan 0,56%. Plot LJ memiliki kandungan C- organik dan N total terendah masing-masing yaitu 2,65% dan 0,29%. Parameter tersebut sangat sesuai dengan populasi bakteri tanah keseluruhan dan populasi bakteri selulolitik tanah terbesar pada bulan Juni 2022 dengan nilai 1,71 x 105 CFU/g dan 4,24 x 104 CFU/g, sedangkan bulan Juli 2022 sebesar 1,81 x 105 CFU/g dan 3,96 x 104 CFU/g. Plot LJ memiliki nilai terendah pada bulan Juni masing-masing sebesar 0,125 x 105 CFU/g dan 1,35 x 104 CFU/g, sedangkan bulan Juli sebesar 0,125 x 105 CFU/g dan 1,43 x 104 CFU/g. Nilai berat kering serasah in situ pada petak PK lebih besar dibandingkan dengan petak PS dan LJ sebesar 81% dan 87%. Berdasarkan hasil tersebut, dapat disimpulkan bahwa perubahan pengelolaan yang berbeda dapat mempengaruhi kondisi kimia tanah dan perubahan populasi total bakteri tanah dan bakteri tanah seluloliti
English Abstract
Different management of agroforestry systems changes crop composition and growth. This will affect organic matter inputs that become the source of food and energy for soil microorganisms. The soil organisms themself play an important role in amplifying soil biogeochemical processes and regulating soil reactions. This process allows the nutrients to be released into the soil and absorbed by the crop. Therefore, this study aims to add information about the abundance of cellulolytic bacteria, the activity of cellulolytic bacteria isolates found, and to analyze the relationship of various land use that affect the quality of litter to the abundance of cellulolytic bacteria in the UB Forest area. The research will be conducted in UB Forest from February to June 2022. The analysis of cellulolytic bacteria will be held at Microbiology Laboratory of the Islamic University of Malang. Laboratory analysis will be held at the Laboratory of the Department of Soil, Faculty of Agriculture, Brawijaya University. The study was conducted using a survey method in various land uses in the UB Forest area which are differentiated based on land cover. The design used in this study was a randomized completed block design (RCBD). The observation plot points were pine-coffee agroforestry (PK), pine-banana agroforestry (PPs), pine-spice agroforestry (PR), pine-vegetable agroforestry (PS/control), mixed gardens (KC) and citrus fields (LJ). There are 6 observation plot points with 4 replications in different locations, so the total research plot is 24 plots (20 m x 20 m). The variables measured in this study included soil biology (total bacterial population, total population of cellulolytic bacteria and cellulolytic bacterial activity), soil chemistry (pH, organic C, total N), soil organic matter (lignin content, polyphenols, wet and dry weight of litter), litter quality (C/N ratio) and litter thickness), vegetation (stem diameter and canopy cover), and microclimate (soil temperature, air temperature and humidity). Data analysis used Analysis of Variance (ANOVA) at 5% level, followed by BNJ test to analyze the differences between treatments.In order to determine the relationship and influence between variables using regression and correlation tests. The results showed that the highest organic C and N contents were obtained in the PK plots (agroforestry of 41 year old pine and 11 year old coffee) which were around 6.64% and 0.56%. The LJ plot had the lowest C-organic and total N contents of 2.65% and 0.29%, respectively. These parameters are in good agreement with the population of soil bacteria and the largest population of soil cellulolytic bacteria in June 2022 with values of 1.71 x 105 CFU/g and 4.24 x 104 CFU/g, while in July 2022 it was 1.81 x 105 CFU/g. g and 3.96 x 104 CFU/g. The LJ plot has the lowest values in June of 0.125 x 105 CFU/g and 1.35 x 104 CFU/g, while in July it is 0.125 x 105 CFU/g and 1.43 x 104 CFU/g. In situ litter dry weight values in the PK plot were greater than the PS and LJ plots of 81% and 87%. Based on the results, it can be concluded that different management changes can affect soil chemical conditions and changes in the total population of soil bacteria and cellulolytic soil bacteria.
Other obstract
-
| Item Type: | Thesis (Magister) |
|---|---|
| Identification Number: | 0423040003 |
| Subjects: | 600 Technology (Applied sciences) > 630 Agriculture and related technologies |
| Divisions: | S2/S3 > Magister Pengelolaan Tanah dan Air, Fakultas Pertanian |
| Depositing User: | Nur Cholis |
| Date Deposited: | 02 Aug 2023 01:49 |
| Last Modified: | 02 Aug 2023 01:49 |
| URI: | http://repository.ub.ac.id/id/eprint/202303 |
![[thumbnail of DALAM MASA EMBARGO]](http://repository.ub.ac.id/style/images/fileicons/text.png) |
Text (DALAM MASA EMBARGO)
Maydella Vista Putri R.pdf Restricted to Registered users only until 31 December 2025. Download (8MB) |
Actions (login required)
 |
View Item |