Azizah, Syafiatul and Prof. Dr. dr. Loeki Enggar Fitri, M.Kes., Sp.ParK. and Prof. Dian Handayani, S.KM., M. Kes., PhD. (2022) Pengaruh Pemberian Probiotik Lactobacillus Casei Dan Bifidobacterium Longum Terhadap Profil Lactobacillus Spp. Dan Bifidobacterium Spp. Intestinal Mencit C57bl/6 Yang Diinfeksi Plasmodium Berghei. Magister thesis, Universitas Brawijaya.
Abstract
Infeksi Plasmodium menyebabkan kondisi disbiosis yaitu kondisi terjadi ketidakseimbangan mikrobiota usus di dalam tubuh manusia yang menjadi penyebab keparahan penyakit malaria. Infeksi Plasmodium menyebabkan perubahan penurunan filum Firmicutes, Lactobacillaceae peningkatan Proteobacteria dan Verrucomicrobia, yang berhubungan dengan derajat parasitemia dan skor malaria serebral. Eritrosit yang tersekuestrasi kedalam pembuluh darah jaringan usus menyebabkan mastositosis usus selama infeksi, menyebabkan peningkatan histamin ileum dan plasma sehingga terjadi inflamasi dan kerusakan epitel usus yang dapat menyebabkan invasi bakteri patogen dan mengganggu keseimbangan mikrobiota usus. Pemberian probiotik Lactobacillus casei dapat meningkatkan diferensiasi Th1 dan mengurangi parasitemia melalui stimulasi sistem imun. Probiotik Bifidobacterium longum dapat mempengaruhi respon imun dan meregulasi sel T regulator serta terbukti dapat meningkatkan bakteri baik di usus. Pemberian probiotik spesies Lactobacillus dan Bifidobacterium dapat menurunkan beban parasitemia dan memperbaiki komposisi mikrobiota usus pada tikus dengan infeksi Plasmodium. Penelitian ini bertujuan mengetahui pengaruh probiotik L. casei dan B. longum terhadap mikrobiota usus Lactobacillus spp. dan Bifidobacterium spp. mencit galur C57BL/6 yang diinfeksi Plasmodium berghei. Terdapat 5 kelompok perlakuan yaitu kontrol negatif, kontrol positif, kelompok intervensi L. casei, kelompok intervensi B. longum dan kelompok intervensi kombinasi L. casei dan B longum. Intervensi probiotik diberikan sebanyak 109 CFU dalam 100 l / hari pada 5 hari sebelum infeksi hingga hari pembedahan. Pada Penelitian ini dilakukan pengukuran pada time point 1, 2 dan 3. Hasil analisis statistik bakteri Lactobacillus spp. menunjukkan pada time point kedua (p=0,003), uji statistik pos hoc pada kelompok negatif perlakuan kombinasi (p=0,040), kontrol positif dengan perlakuan kombinasi (p=0,009) dan perlakuan L. casei dengan perlakuan kombinasi (p=0,009). Analisis statistik jumlah bakteri Bifidobacterium spp. didapatkan bahwa hasil uji statistik Kruskall wallis didapatkan hasil pada TP 1, TP 2, TP 3 masing masing secara berurutan (p=1,000), (p=0,066), (p=0,206). Sedangkan pada uji pengaruh L. casei dan B. longum terhadap derajat parasitemia didapatkan hasil (p=0,264). Pemberian probiotik B. longum dapat menghambat peningkatan derajat parasitemia dan meningkatkan mikrobiota usus genus Lactobacillus spp. dan Bifidobacterium spp. sebelum dan sesudah infeksi.
English Abstract
Plasmodium infection causes a dysbiosis which cause of the severity of malaria. Plasmodium infection caused dysbiosis that decrease Firmicutes phylum, Lactobacillaceae and increase in Proteobacteria, Verrucomicrobia which related to the degree of parasitemia and increase of cerebral malaria incidents. Sequestration of malaria into the blood vessels of intestinal tissue cause intestinal mastocytosis during infection, influence in ileal plasma histamin, resulting in inflammation and damage to the intestinal epithelium that can lead to invasion of pathogenic bacteria and bleeding. This condition can disrupt the balance of the intestinal microbiota and correlate with a increase in the degree of parasitemia. The new paradigm suggest that the immune system has evolved to accommodate colonization of the gut microbiota under homeostatic conditions against malaria parasites. Administration of probiotic Lactobacillus casei can increase Th1 differentiation and reduce parasitemia through stimulation of the immune system. The probiotic Bifidobacterium longum can influence the immune response and regulate regulatory T cells and has been shown to increase good bacteria in the gut. Probiotic administration can reduce the burden of parasitemia and improve the gut microbiota in rats with Plasmodium infection. This study aimed to determine the effect of probiotic Lactobacillus casei and Bifidobacterium longum on Lactobacillus spp. and Bifidobacterium spp. profile in mice infected with Plasmodium berghei. There were five groups, negative control, positive control, L. casei group, B. longum group and kombinasi (L. casei and B. longum group). Probiotic intervention was given as much as 109 CFU / day before and after infection. This study gut microbiota measured in time point 1, 2 and 3. Results of statistical analysis of Lactobacillus spp. showed a significant differences at the second time point (p=0,003), post hoc test showed that there was a significant differences in the negative group with kombinasi group (p=0,040), positive group with kombinasied group (p=0,009) and L. casei group with kombinasi group (p=0,009). Kruskall Wallis test in Bifidobacterium spp. genus TP 1, TP 2, TP 3 respectively (p=1.000), (p=0,066), (p=0,206). The result of the effect of L. casei and B. longum and the degree of parasitemua showed (p=0,264),. Oral administration of B. longum probiotics has been shown to inibit the degree of parasitemia and increase gut microbiota Lactobacillus spp. and Bifidobacterium spp. genus before and after infection.
Other obstract
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| Item Type: | Thesis (Magister) |
|---|---|
| Identification Number: | '0422060194 |
| Subjects: | 600 Technology (Applied sciences) > 616 Diseases > 616.02 Special topics of disease > 616.025 Medical emergencies / Emergency medicine / Emergency nursing / Triage (Medicine) |
| Divisions: | S2/S3 > Magister Ilmu Biomedis, Fakultas Kedokteran |
| Depositing User: | Endang Susworini |
| Date Deposited: | 17 Jul 2023 08:28 |
| Last Modified: | 17 Jul 2023 08:28 |
| URI: | http://repository.ub.ac.id/id/eprint/201904 |
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