Sakti, Pandu Tridana and Dr. dr. Nur Samsu, SpPD, K-GH and dr. Achmad Rifai, SpPD, K-GH (2023) Efek Pemberian Candesartan Dibandingkan Losartan Terhadap Penghambatan Ekspansi Mesangial Dan Penurunan Volume Glomerulus Pada Tikus Model Diabetes Yang Mendapatkan Asam Rosmarinat. Magister thesis, Universitas Brawijaya.
Abstract
Pendahuluan: Stres oksidatif dan aktivasi renin angiotensin system (RAS) akibat kondisi hiperglikemia kronis merupakan patogenesis dari nefropati diabetik (ND). Pemberian antioksidan seperti asam rosmarinat (AR) mampu menurunkan progesivitas ND, meskipun hasilnya belum memuaskan akibat ketidakmampuan AR dalam menghambat angiotensin II reseptor tipe 1. Penambahan angiotensin II reseptor blocker (ARB) dengan AR diharapkan mampu memberikan efek sinergitas dalam mencegah progesivitas ND melalui penghambatan RAS. Tujuan: Mengetahui efek penghambatan ekspansi mesangial dan penurunan volume glomerulus pada tikus model diabetes setelah diberikan kombinasi AR dengan ARB (candesartan atau losartan). Metode: Desain true experimental laboratory dengan metode randomized post-test only control group pada tikus model diabetik yang diberikan diet tinggi lemak dan injeksi streptozotocin. Tikus dibagi kedalam 4 kelompok yang masing-masing terdiri dari 4 tikus: kontrol negatif; kontrol positif; perlakuan AR dan candesartan; perlakuan AR dan losartan. Ekspansi mesangial dan volume glomerulus dinilai setelah 8 minggu perlakuan. Analisis data dengan uji One-way ANOVA dan post hoc Tukey. Hasil: Kelompok perlakuan AR + candesartan secara signifikan menghambat ekspansi mesangial (MD: [-19.92]; 95%CI: [-21.97], [-17.87]; p: 0.000) dan penurunan volume glomerulus (MD: [2669.06]; 95%CI: [2066.31], [3271.80]; p: 0.000) dibandingkan kontrol positif. Demikian pula kelompok perlakuan AR + losartan secara signifikan menghambat ekspansi mesangial (MD: [-19.21]; 95%CI: [-21.26], [-17.16]; p: 0.000) dan penurunan volume glomerulus (MD: [2488.04]; 95%CI: [1885.29], [3090.78]; p: 0.000) dibandingkan kontrol positif. Kesimpulan: Kombinasi asam rosmarinat dengan candesartan atau losartan memiliki efek penghambatan ekspansi mesangial dan penurunan volume glomerulus pada tikus diabetes.
English Abstract
Background: Oxidative stress and activation of the renin-angiotensin system (RAS) are the pathogenesis of diabetic nephropathy (DN). Rosmarinic acid (RA) can reduce the progression of ND, although the results are not satisfactory due to the inability to inhibit angiotensin II receptor type 1. The addition of angiotensin receptor blockers (ARB) with RA is expected to provide synergistic effect in preventing the progression of ND. Aims: To determine the effect of inhibiting mesangial expansion and decrease glomerular volume in diabetic rats after being given a combination of AR with ARB (candesartan or losartan). Methods: A true experimental laboratory design with a randomized post-test only control group. Sixteen rats were divided into 4 groups: negative control; positive control; RA and candesartan treatment; RA and losartan treatment. Mesangial expansion and glomerular volume were assessed after 8 weeks of treatment. Data analysis with One-way ANOVA and Tukey's post hoc tests. Results: The RA + candesartan treatment group significantly inhibited mesangial expansion (MD: [-19.92]; 95%CI: [-21.97], [-17.87]; p: 0.000) and decrease glomerular volume (MD: [2669.06]; 95 %CI: [2066.31], [3271.80]; p: 0.000) compared to the positive control. Likewise the RA + losartan treatment group significantly inhibited mesangial expansion (MD: [-19.21]; 95%CI: [-21.26], [-17.16]; p: 0.000) and decrease glomerular volume (MD: [2488.04]; 95 %CI: [1885.29], [3090.78]; p: 0.000) compared to the positive control. Conclusion: The combination of rosmarinic acid with candesartan or losartan has the effect of inhibiting mesangial expansion and decrease glomerular volume in diabetic rats.
Other obstract
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| Item Type: | Thesis (Magister) |
|---|---|
| Identification Number: | '0423060006 |
| Uncontrolled Keywords: | Asam Rosmarinat, Candesartan, Ekspansi Mesangial, Losartan, Volume Glomerulus,-Candesartan, Glomerular Volume, Losartan, Mesangial Expansion, Rosmarinic Acid. |
| Subjects: | 600 Technology (Applied sciences) > 616 Diseases |
| Divisions: | Profesi Kedokteran > Spesialis Ilmu Penyakit Dalam, Fakultas Kedokteran |
| Depositing User: | Endang Susworini |
| Date Deposited: | 17 Jul 2023 05:10 |
| Last Modified: | 17 Jul 2023 05:10 |
| URI: | http://repository.ub.ac.id/id/eprint/201891 |
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