Andriani, Nurvia and dr. Zamroni Afif, Sp.S (K), M. Biomed and dr. Rodhiyan Rakhmatiar, Sp.S (K), FINA, M. Biomed and dr. Holipah, Ph.D (2023) Pengaruh Ekstrak Pegagan (Centella Asiatica) Terhadap Ekspresi Deacetylase Sirtuin-1 (Sirt1) Dan Period Circadian Regulator-2 (Per2) Pada Larva Ikan Zebra (Danio Rerio) Model Insomnia. Magister thesis, Universitas Brawijaya.
Abstract
Latar Belakang : Insomnia didefinisikan sebagai kesulitan untuk memulai tidur, mempertahankan tidur, atau kondisi tidur yang tidak mampu merestorasi kondisi tubuh dan mental dengan adekuat. Namun terapi insomnia belum adekuat untuk memperbaiki kondisi insomnia. Terdapat beberapa pilihan terapi alternatif untuk terapi insomnia, misalnya salah satu yang terkenal adalah daun pegagan (Centella asiatica). Banyak jalur yang menyebabkan regulasi tidur, termasuk irama sirkardian dan reaksi seluler. Dibutuhkan penelitian untuk memperbaiki kondisi insomnia melalui jalur irama sirkadian dengan pemberian Centella asiatica dengan pengamatan pada ikan zebra model insomnia. Tujuan Penelitian : Membuktikan pemberian ekstrak Pegagan (Centella asiatica) mempengaruhi jalur irama sirkadian melalui ekspresi SIRT1 dan Per2 pada larva ikan zebra (Danio rerio) model insomnia. Metode dan Sampel Penelitian : Studi true laboratory experimental dengan pendekatan randomized control group post test only, dengan sampel 50 larva ikan zebra (Danio rerio) model insomnia yang diberikan perlakuan ekstrak pegagan (Centella asiatica) konsentrasi 2.5 μg/mL, 5 μg/mL dan 10 μg/mL yang dilakukan di Laboratorium Farmakologi dan Laboratorium Biomedik Fakultas Kedokteran Universitas Brawijaya Malang selama 3 bulan, yakni pada bulan September hingga November 2022. Hasil Penelitian : Dari penelitian ini didapatkan bahwa terdapat pengaruh yang dari pemberian ekstrak pegagan terhadap ekspresi SIRT1 (p=0.001), dengan ekstrak pegagan dengan konsentrasi 10 μg/mL paling optimal meningkatkan ekspresi SIRT1 pada kelompok larva ikan Zebra model insomnia. Tidak terdapat pengaruh yang dari pemberian ekstrak pegagan terhadap ekspresi Per2 pada kelompok larva ikan Zebra model insomnia (p=0.051). Kesimpulan : Pemberian ekstrak pegagan (Centella asiatica) pada konsentrasi 10 μg/mL dapat meningkatkan ekspresi Deacetylase Sirtuin-1 (SIRT1) tetapi tidak memiliki pengaruh terhadap ekspresi Per2 pada larva ikan zebra model insomnia.
English Abstract
Background : Insomnia is defined as difficulty falling asleep, maintaining sleep, or sleeping conditions that are unable to adequately restore the body and mental state. However, insomnia therapy has not been adequate to improve the condition of insomnia. There are several alternative therapy options for treating insomnia, for example one that is well known is Pegagan leaves (Centella asiatica). Many pathways lead to regulation of sleep, including circadian rhythms and cellular reactions. Research is needed to improve the condition of insomnia through circadian rhythms by administering Centella asiatica with observations on zebrafish models of insomnia. Objectives : Proving that administration of Centella asiatica extract influences the circadian rhythms pathway through SIRT1 and Per2 expression in zebrafish larvae (Danio rerio) insomnia model. Material and Methods : A true laboratory experimental study with a randomized control group post test only approach, with a sample of 50 zebrafish larvae (Danio rerio) insomnia model that was given treatment with Centella asiatica extract at concentrations of 2.5 μg/mL, 5 μg/mL and 10 μg/mL which carried out at the Pharmacology Laboratory and the Biomedical Laboratory, Faculty of Medicine, Brawijaya University, Malang for 3 months, from September to November 2022. Results : This study found a significant effect of having Centella Asiatica extract on the expression of SIRT1 (p=0.001), with Centella Asiatica extract at concentrations of 10 μg/mL elevating SIRT1 expression in the group of zebrafish larvae with the insomnia model. There was no significant effect of having Centella Asiatica extract on Per2 expression in the group of zebrafish larvae with the insomnia model (p=0.051). Conclusion : Administration of Centella asiatica extract at a concentration 10 μg/mL can elevate the expression of SIRT1 and have no significant effect on Per2 expression in zebrafish larvae with insomnia models.
Other obstract
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| Item Type: | Thesis (Magister) |
|---|---|
| Identification Number: | '0423060005 |
| Uncontrolled Keywords: | Insomnia, Ekstrak pegagan (Centella asiatica), ikan zebra (Danio rerio), SIRT1, Per2, Irama sirkadian,-Insomnia, Centella asiatica extract, Zebrafish larvae (Danio rerio), SIRT1, Per2, Circadian rhythms |
| Subjects: | 600 Technology (Applied sciences) > 616 Diseases > 616.8 Diseases of nervous system and mental disorders |
| Divisions: | Profesi Kedokteran > Spesialis Neurologi, Fakultas Kedokteran |
| Depositing User: | Endang Susworini |
| Date Deposited: | 17 Jul 2023 04:16 |
| Last Modified: | 17 Jul 2023 04:16 |
| URI: | http://repository.ub.ac.id/id/eprint/201885 |
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