Hafiz, Farah Betari and Ningrum Paramita Sari,, S.Keb.Bd, M.Biomed and dr. Andrew William Tulle, M.Sc. (2022) Uji Efek Antibakteri Ekstrak Etanol Kulit Bawang Merah (Allium ascalonicum L.) terhadap Streptococcus agalactiae secara In Vitro. Sarjana thesis, Universitas Brawijaya.
Abstract
Grup B Streptococcus (GBS) juga dikenal sebagai Streptococcus agalactiae berkolonisasi pada vagina dan saluran pencernaan pada 15-45% wanita sehat, dengan potensi infeksi intrauterin asendens atau penularan selama persalinan yang dapat menyebabkan neonatus berisiko terkena infeksi. Resistensi Streptococcus agalactiae terhadap beberapa kelas antibiotik menyulitkan pemberian terapi kepada pasien yang telah terinfeksi. Kulit bawang merah (Allium ascalonicum L.) diketahui memiliki kandungan senyawa aktif yang bersifat antibakteri seperti saponin, alkaloid, flavonoid, dan tanin. Tujuan dari penelitian ini untuk mengetahui efek antibakteri ekstrak etanol kulit bawang merah (Allium ascalonicum L.) terhadap Streptococcus agalactiae secara in vitro. Penelitian ini dilakukan dengan well diffusion method dengan konsentrasi ekstrak etanol kulit bawang merah sebesar 2%, 4%, 6%, 8%, 10% serta kontrol positif berupa antibiotik penisilin konsentrasi 0,0075 μg/ml dan kontrol negatif berupa aquades dengan pengulangan sebanyak 5 kali. Penelitian menunjukkan terbentuknya zona hambat pada pemberian ekstrak etanol kulit bawang merah pada konsentrasi 2%, 4%, 6%, 8%, 10% dan kontrol positif Penisilin dengan rata – rata zona hambat secara berurutan sebesar 10 mm, 11,5 mm, 11,95 mm, 12,35 mm, 12,95 mm dan 15 mm. Hasil uji non parametrik Kruskal-Wallis didapatkan nilai p < 0.05 menunjukkan perbedaan signifikan antara seluruh kelompok perlakuan terhadap rerata diameter zona hambat. Dampat disimpulkan ekstrak etanol kulit bawang merah (Allium ascalonicum L.) memiliki efek sebagai antibakteri terhadap Streptococcus agalactiae secara in vitro.
English Abstract
Group B Streptococcus (GBS) is also known as Streptococcus agalactiae colonizes the vaginal and digestive tract in 15-45% of healthy women, with potential for ascending intrauterine infection or transmission during parturition, which can creating the neonate at risk of infection. Streptococcus agalactiae resistance against several classes of antibiotics cause difficulty in choosing therapy for patients who have been infected. Red onion skin leaves (Allium ascalonicum L.) are known to have antibacterial active compounds such as saponins, alkaloids, flavonoids and tanins. The purpose of this study was to determine the antibacterial effect of ethanolic extract of red onion skin (Allium ascalonicum L.) against Streptococcus agalactiae in vitro. The study used the well diffusion method with a concentration of ethanol extract of red onion skin of 2%, 4%, 6%, 8%, 10%, aquadest as a negative control and 0.0075 μg/ml antibiotic Penicillin as a positive control, each treatment was repeated five times. The results showed the presence of inhibition zones at each concentration with average diameter at the concentrations of 2%, 4%, 6%, 8%, 10% and positive control were 10 mm, 11.5 mm, 11.95 mm, 12.35 mm, 12.95 mm and 15 mm respectively. The results of the non-parametric Kruskal-Wallis test obtained p value <0.05, indicating a significant difference between all treatment groups to the average diameter of the inhibition zone. As conclusion, the ethanolic extract of red onion skin has potential as antibacterial effect against Streptococcus agalactae in vitro.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | 0522060226 |
| Uncontrolled Keywords: | Antibakteri, Streptococcus agalactiae, kulit bawang merah,Antibacterial, Streptococcus agalactiae, Red Onion Skin |
| Subjects: | 600 Technology (Applied sciences) > 618 Gynecology, obstetrics, pediatrics, geriatrics > 618.2 Obstetrics |
| Divisions: | Fakultas Kedokteran > Kebidanan |
| Depositing User: | Budi Wahyono Wahyono |
| Date Deposited: | 26 May 2023 02:16 |
| Last Modified: | 26 May 2023 02:16 |
| URI: | http://repository.ub.ac.id/id/eprint/200392 |
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