Barazani, Hero and Prof. Dr. dr. Loeki Enggar Fitri,, M. Kes, Sp. ParK (2023) Uji Antimaaria Fraksi Metabolit Sekunder Streptomyces hygroscopicus subsp. Hygroscopicus Isolat Indonesia dalam Menghambat Pertumbuhan Parasit secara In Vitro Menggunakan LDH Assay. Sarjana thesis, Universitas Brawijaya.
Abstract
Pemberantasan kasus malaria di Indonesia masih belum tercapai yang terbukti adanya peningkatan Angka Kesakitan Malaria pada tahun 2018 ke tahun 2019. Penanganan malaria memiliki berbagai hambatan, salah satunya resistensi obat antimalaria di Indonesia bahkan Asia Tenggara. Penelitian terdahulu membuktikan ekstrak metabolit sekunder Streptomyces hygroscopicus isolat Indonesia mempunyai efek antimalaria secara in vivo dan in vitro. Penelitian eksperimental ini bertujuan membuktikan efektivitas fraksi 45 - 49 S. hygroscopicus yang belum diteliti sebagai agen antimalaria efektif. Penelitian menggunakan kultur in vitro Plasmodium berghei untuk uji efektivitas dengan metode LDH Assay. Untuk menentukan efek antimalaria metabolit sekunder S hygroscopicus, kultur P. berghei dipapar fraksi 45 – 49 dosis 0.05, 0.5, 5, 50, dan 500 mg/L. Hasil LDH assay menunjukkan fraksi 49 yang berpotensi sebagai antimalaria dibandingkan fraksi nomor lain dengan kadar LDH dosis 5 mg/L lebih rendah dibanding kontrol (p=0,000).Berdasarkan uji statistik Post-Hoc Tukey HSD, pada fraksi 49 terlihat bahwa dosis 0.05 mg/L berbeda signifikan dengan Artemisinin (p=0,000), sedangkan fraksi 49 dimulai pada dosis 0.5, 5, 50, dan 500 mg/L secara berurutan tidak memiliki perbedaan yang signifikan terhadap Artemisinin (p=0,077; p=0,565; p=0,998; p=1,000). Maka dapat disimpulkan fraksi 49 metabolit sekunder S. hygroscopicus memiliki efek antimalaria yang sama seperti Artemisinin.
English Abstract
Eradication of malaria cases in Indonesia has yet to be achieved as evidenced by an increase in malaria morbidity rates from 2018 to 2019. The management of malaria has various obstacles, one of which is the resistance of antimalarial drugs in Indonesia and even Southeast Asia. Previous studies have proven that the Indonesian isolate Streptomyces hygroscopicus secondary metabolite extract has antimalarial effects in vivo and in vitro. This experimental study aims to prove the effectiveness of the 45 - 49 fraction of S. hygroscopicus which has not been studied as an effective antimalarial agent. This study used Plasmodium berghei in vitro culture to test the effectiveness of the LDH assay method. To determine the antimalarial effect of S. hygroscopicus secondary metabolites, P. berghei cultures were exposed to fractions 45 – 49 doses of 0.05, 0.5, 5, 50, and 500 mg/L. The results of the LDH assay showed that fraction 49 had potential as an antimalarial compared to other fraction numbers with LDH levels at a dose of 5 mg/L lower than the control (p=0.000). Based on the Tukey HSD Post-Hoc statistical test, 49 fraction of S. hygroscopicus secondary metabolites showed that the dose of 0.05 mg/L L was significantly different from Artemisinin (p=0.000), whereas fraction 49 started at doses of 0.5, 5, 50, and 500 mg/L respectively did not have a significant difference to Artemisinin (p=0.077; p=0.565; p=0.998; p=1,000). It could be concluded that 49 fraction of S. hygroscopicus secondary metabolites has antimalaria activity similar to those of Artemisinin.
Other obstract
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| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | 0522060297 |
| Uncontrolled Keywords: | Antimalaria, LDH Assay, Plasmodium berghei, metabolit sekunder, Streptomyces hygroscopicus |
| Subjects: | 600 Technology (Applied sciences) > 610 Medicine and health |
| Divisions: | Fakultas Kedokteran > Pendidikan Dokter |
| Depositing User: | Endang Susworini |
| Date Deposited: | 08 May 2023 01:18 |
| Last Modified: | 08 May 2023 01:18 |
| URI: | http://repository.ub.ac.id/id/eprint/198795 |
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