Salsabila, Yofa and apt. Uswatun Khasanah, , S.Farm., M.Farm and apt. Bachtiar Rifai Pratita Ihsan., ,S.Farm.,M.Farm (2022) Penentuan Indeks Selektivitas Antimalaria Ekstrak N-Hek- sana Batang Kayu Songga (Strychnos lucida) Terhadap Plasmodium falciparum 3D7 Sensitif Klorokuin Secara In-vitro. Sarjana thesis, Universitas Brawijaya.
Abstract
Batang Kayu Songga (Strychnos lucida) merupakan salahsatu tanaman obat yang secara empiris telah digunakan oleh masyarakat sebagai obat berbagai macam jenis penyakit salah satunya sebagai antimalaria. Penelitian ini bertujuan untuk mengetahui potensi aktivitas antimalaria dan toksisitas dari Batang Kayu Songga (Strychnos lucida) terhadap Plasmodium falciparum dan sel normal (Vero cell line). Kayu Songga diekstraksi menggunakan n-heksana. Masing-masing seri konsentrasi ekstrak Batang Kayu Songga (100; 10; 1; 0,1; 0,01 μg/mL) dan klorokuin (100; 10; 1; 0,1; 0,01; 0,001 ; 0,0001 μg/mL) diuji aktivitas antimalaria terhadap Plasmodium falciparum dan uji toksisitas terhadap sel Vero (ginjal monyet hijau Afrika) dengan seri konsentrasi (0,01; 0,1; 1; 10; 100; 0,01 μg/mL) menggunakan metode MTT. Aktivitas antimalaria Batang Kayu Songga (Strychnos lucida) dan Klorokuin dalam menghambat Plasmodium falciparum berdasarkan nilai IC50 berturut-turut yaitu 0,016 dan 0,000 μg/mL. Tidak terdapat perbedaan antara aktivitas antimalaria ekstrak n-heksana Batang Kayu Songga (Strychnos lucida) dengan klorokuin (t test, p = 0,118). Selain itu, Batang Kayu Songga (Strychnos lucida) memilki perbedaan toksisitas terhadap sel Vero (ginjal monyet hijau Afrika) dengan nilai CC50 247,26 μg/mL, serta memiliki nilai SI sebesar 15.454,315 μg/mL. Ekstrak n-heksana batang kayu songga dinyatakan selektif dalam menghambat pertumbuhan Plasmodium falciparum dan aman terhadap sel normal nilai SI > 10 .
English Abstract
Batang Kayu Songga (Strychnos lucida) is one of the medicinal plants that empirically has been used by the community as a medicine for various types of diseases, one of which is antimalarial. The purpose of this study was to analyze the potential antimalarial activity and toxicity of Batang Kayu (Strychnos lucida) against Plasmodium falciparum and normal cells (Vero cell line). Batang Kayu Songga (Strychnos lucida) were extracted using n-hexane. Each series concentration of Songga Kayu Trunk extract (100; 10; 1; 0.1; 0.01 g/mL) and chloroquine (100; 10; 1; 0.1; 0.01; 0.001; 0.0001 g /mL) were tested for antimalarial activity against Plasmodium falciparum and for toxicity against Vero cells with concentration series (0.01; 0.1; 1; 10; 100; 0.01 g/mL) using the MTT method. The antimalarial activity Batang Kayu Songga (Strychnos lucida) and Chloroquine in inhibiting Plasmodium falciparum based on IC50 values were 0.016 and 0.000 μg/mL, respectively. There was no difference between the antimalarial activity of the n-hexane extract of Batang Kayu Songga (Strychnos lucida) and chloroquine (t test, p = 0.118).In addition, Batang Kayu Songga (Strychnos lucida) has different toxicity to Vero cells (African green monkey) with a CC50 value of 247,26 μg/mL, and an SI value of 15,454.315 μg/mL. The n-hexane extract of Batang Kayu Songga (Strychnos lucida) was found to be selective in inhibiting the growth of Plasmodium falciparum and safe against normal cells with SI values > 10 .
Other obstract
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| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | 0522060238 |
| Uncontrolled Keywords: | Kayu Songga (Strychnos lucida), antimalaria, ekstrak n-heksana, toksisitas, Plasmodium falciparum, dan sel Vero (ginjal monyet hijau Afrika) |
| Subjects: | 600 Technology (Applied sciences) > 615 Pharmacology and therapeutics > 615.1 Drugs (materia medica) |
| Divisions: | Fakultas Kedokteran > Farmasi |
| Depositing User: | Endang Susworini |
| Date Deposited: | 03 May 2023 02:06 |
| Last Modified: | 03 May 2023 02:06 |
| URI: | http://repository.ub.ac.id/id/eprint/198470 |
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