Kasilani, Dharmapadmi Pradnya and Dr. Yuli Nugraeni, drg., Sp.KG (2022) Uji Toksisitas Senyawa pada Daun Tanaman Alamanda (Allamanda cathartica L.) secara In Silico sebagai Antibakteri pada Bahan Irigasi Saluran Akar. Sarjana thesis, Universitas Brawijaya.
Abstract
Daun Alamanda mengandung senyawa turunan yang berkhasiat sebagai antimikroba, meliputi β-sitosterol, β�amyrin, phytol, ursolic acid, allamandin, plumericin, isoplumericin, iridoid, sesquiterpenes, dan plumeride. Prosedur perawatan saluran akar tentu membutuhkan bahan irigasi saluran akar. Namun bahan irigasi saluran akar yang digunakan saat ini masih memiliki kelemahan, contohnya sifat sitotoksisk dan kaustik pada NaOCl. Tujuan penelitian ini, menganalisis toksisitas senyawa turunan yang terkandung dalam daun Alamanda dengan uji in silico. Metode yang digunakan merupakan penelitian eksperimental berbasis komputasi. Senyawa uji, dalam bentuk data Canonical SMILE yang diunduh pada database PubChem Compound, diuji toksisitasnya menggunakan program pKCSM. Hasil uji diinterpretasi berdasarkan jurnal berjudul pkCSM: Predicting Small�Molecule Pharmacokinetic and Toxicity Properties Using Graph-Based Signatures oleh Pires dkk (2015). Hasil uji menunjukkan, β-sitosterol berpotensi menjadi inhibitor hERG II, toksik pada prediktor T. Pyriformis toxicity, dan toksisitas akut tinggi; begitu pula β-amyrin. Phytol berpotensi sebagai inhibitor hERG II, menyebabkan alergi kulit, toksik pada prediktor T. Pyriformis toxicity, dan toksisitas akut tinggi. Ursolic acid berpotensi menyebabkan gangguan fungsi hati, toksik pada prediktor T. Pyriformis toxicity, dan toksisitas akut tinggi. Allamandin berpotensi menjadi mutagenik dan toksik pada prediktor T. Pyriformis toxicity. Plumericin memiliki potensi sebagai mutagenik dan toksik pada prediktor T. Pyriformis toxicity; begitu pula isoplumericin. Iridoid berpotensi toksik pada prediktor T. Pyriformis toxicity; begitu pula plumierida. Sesquiterpene berpotensi toksik pada prediktor T. Pyriformis toxicity dan menyebabkan sensitifitas kulit. Dari hasil uji, apabila senyawa uji dijadikan obat maka perlu dilakukan uji laboratorium terlebih dahulu sebelum diujikan pada manusia.
English Abstract
Alamanda leaves contain derivative compounds that have antimicrobial properties, including β-sitosterol, β�amyrin, phytol, ursolic acid, allamandin, plumericin, isoplumericin, iridoids, sesquiterpenes, and plumeride. Root canal treatment procedures certainly require root canal irrigation materials. However, the root canal irrigant currently used still has weaknesses, for example, the cytotoxic and caustic properties of NaOCl. The purpose of this study was to analyze the toxicity of derivative compounds contained in Alamanda leaves with the in silico test. The method used is computational-based experimental research. The test compound, in the form of Canonical SMILE data (from PubChem Compound database), was tested for its toxicity using the pKCSM program. The test results are interpreted based on a journal entitled pkCSM: Predicting Small�Molecule Pharmacokinetic and Toxicity Properties Using Graph-Based Signatures by Pires et al. (2015). The test results showed that β-sitosterol has the potential to be an inhibitor of hERG II, toxic to (T. Pyriformis toxicity predictor), and high acute toxicity; as well as β-amyrin. Phytol has potential as an hERG II inhibitor, causing skin allergies, toxic (T. Pyriformis toxicity predictor), and high acute toxicity. Ursolic acid has the potential to cause impaired liver function, toxic (T. Pyriformis toxicity predictor), and is of high acute toxicity. Allamandin is potentially mutagenic and toxic (T. Pyriformis toxicity predictor). Plumericin has the potential to be mutagenic and toxic (T. Pyriformis toxicity predictor); as well as isoplumericin. Iridoids are potentially toxic (T. Pyriformis toxicity predictor); as well as plumieride. Sesquiterpenes are potentially toxic (T. Pyriformis toxicity predictor) and cause skin sensitivity. From the test results, if the test compound is used as a drug, it is necessary to carry out laboratory tests before testing it on humans
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | 0522140016 |
| Uncontrolled Keywords: | Allamanda cathartica L.; in silico; irigasi saluran akar; pKCSM; uji toksisitas, Allamanda cathartica L.; in silico; root canal irrigation; pKCSM; toxicity test. |
| Subjects: | 600 Technology (Applied sciences) > 617 Surgery, regional medicine, dentistry, ophthalmology, otology, audiology > 617.6 Dentistry |
| Divisions: | Fakultas Kedokteran Gigi > Kedokteran Gigi |
| Depositing User: | Zainul Mustofa |
| Date Deposited: | 20 Mar 2023 02:34 |
| Last Modified: | 20 Mar 2023 02:34 |
| URI: | http://repository.ub.ac.id/id/eprint/197703 |
![[thumbnail of DALAM MASA EMBARGO]](http://repository.ub.ac.id/style/images/fileicons/text.png) |
Text (DALAM MASA EMBARGO)
Dharmapadmi Pradnya Kasilani.pdf Restricted to Registered users only until 31 December 2024. Download (3MB) |
Actions (login required)
 |
View Item |