Isnaeni, Miftaqul Fajar and Prof. Dr. Ir. Bambang Dwi Argo,, DEA and Farida Rahayu, , S.Si., M.P., Ph.D (2022) Uji Efektivitas Ekstrak Pepaya (Carica Papaya L.) : Kulit, Biji, Daging Dan Daun Sebagai Antibakteri Untuk Menghambat Bakteri Penyebab Turunnya Kadar Sukrosa Terhadap Tanaman Tebu (Saccharum Officinarum L.). Sarjana thesis, Universitas Brawijaya.
Abstract
Menurunnya kualitas nira tebu pasca panen dapat disebabkan oleh beberapa faktor salah satunya adanya kontaminasi bakteri pendegradasi sukrosa. Kontaminasi oleh bakteri dapat menyebabkan rendahnya rendemen gula dengan kualitas yang menurun sehingga harga jual tebu setelah panen juga mengalami penurunan. Dalam hal ini perlu dilakukan upaya pencegahan untuk mengurangi dampak kerusakan yang diakibatkan oleh bakteri dengan antibiotic alami dari pemanfaatan biomassa disekitar. Penelitian ini bertujuan untuk melakukan ekstraksi maserasi pada tanaman papaya yaitu daging, kulit, biji dan daun papaya, mengetahui potensi aktivitas senyawa antibakteri dari ekstrak daging, kulit, biji, dan daun papaya dengan metode uji difusi cakram dan dilusi cair dengan konsentrasi 25%, 50%, 75%, 85%, 95% serta control positif (kloramfenicol) dan control negative (DMSO), dan mempelajari konsumsi nira oleh bakteri pendegradasi sukrosa. Ekstraksi menghasilkan nilai rendemen ekstrak hasil maserasi secara berturut-turut yaitu ekstrak biji papaya sebesar (78.161%), ekstrak daun papaya sebesar (76.4062%), ekstrak kulit papaya sebesar (74.4162%), dan ekstrak daging papaya sebesar (59.8886%). Melalui analisis Kruskal Wallis dan Post Hoc Mann Whitney didapatkan pengaruh berbeda nyata pada uji difusi cakram dengan ekstrak daging papaya 95% dan 85% memiliki daya hambat paling berpengaruh diikuti dengan ekstrak kulit papaya 85%, sedangkan uji dilusi cair didapatkan nilai KHM 50% (ekstrak daging, kulit, dan biji papaya) dan 25% (ekstrak daun papaya). Sedangkan nilai KBM 50% (ekstrak daging, biji dan daun papaya) dan 85% (ekstrak kulit papaya). Pengamatan perubahan zona hambat dan nilai absorbansi dilakukan selama 7 hari pengamatan. Uji total gula nira tebu pada konsorsium bakteri mampu menyebabkan terjadinya penurunan sukrosa dari 0.78 mg/ml menjadi 0.48 mg/ml selama 7 hari pengamatan
English Abstract
The decline in the quality of post-harvest sugarcane juice can be caused by several factors, one of which is contamination with sucrose-degrading bacteria. Contamination by bacteria can cause low sugar yield with decreased quality so that the selling price of sugarcane after harvest also decreases. In this case, prevention efforts need to be made to reduce the impact of damage caused by bacteria with natural antibiotics from the use of surrounding biomass. This study aims to perform maceration extraction on papaya plants, namely flesh, skin, seeds and leaves of papaya, to determine the potential activity of antibacterial compounds from extracts of flesh, skin, seeds, and leaves of papaya with the disc diffusion test method and liquid dilution with a concentration of 25%, 50 %, 75%, 85%, 95% and positive control (chloramphenicol) and negative control (DMSO), and studied the consumption of sap by sucrose-degrading bacteria. The extraction resulted in the yield value of the macerated extract, respectively, namely papaya seed extract (78.161%), papaya leaf extract (76.4062%), papaya peel extract (74.4162%), and papaya flesh extract (59.8886%). Through the analysis of Kruskal Wallis and Post Hoc Mann Whitney, found that there was a significantly different effect on the disc diffusion test with papaya flesh extract 95% and 85% having the most influential inhibition followed by 85% papaya peel extract, while the liquid dilution test obtained a MIC value of 50% (extract flesh, skin, and seeds of papaya) and 25% (pawpaw leaf extract). While the value of KBM 50% (extract of meat, seeds and leaves of papaya) and 85% (extract of papaya skin). Observations of changes in the inhibition zone and absorbance values were carried out for 7 days of observation. The total test of sugar cane juice on a consortium of bacteria was able to cause a decrease in sucrose from 0.78 mg/ml to 0.48 mg/ml for 7 days of observation.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | 0522100394 |
| Uncontrolled Keywords: | Antibakteri, Ekstraksi, Asam Sulfat-Fenol, Difusi Cakram, Dilusi Cair,Antibacterial, Extraction, Sulfuric Acid-Phenol, Disc Diffusion, Liquid Dilution |
| Subjects: | 600 Technology (Applied sciences) > 630 Agriculture and related technologies |
| Divisions: | Fakultas Teknologi Pertanian > Keteknikan Pertanian |
| Depositing User: | soegeng sugeng |
| Date Deposited: | 31 Jan 2023 02:34 |
| Last Modified: | 31 Jan 2023 02:34 |
| URI: | http://repository.ub.ac.id/id/eprint/197139 |
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