Pengaruh Aplikasi Bakteri Indigenous Ub Forest Terhadap Pertumbuhan Dan Kesehatan Tanaman Tomat Cherry (Solanum Lycopersicum Var. Cerasiforme)

Rahayuningtyas, Ninda and Luqman Qurata Aini, (2022) Pengaruh Aplikasi Bakteri Indigenous Ub Forest Terhadap Pertumbuhan Dan Kesehatan Tanaman Tomat Cherry (Solanum Lycopersicum Var. Cerasiforme). Sarjana thesis, Universitas Brawijaya.

Abstract

Salah satu jenis tomat yang populer di dunia dan banyak diminati masyarakat yakni Tomat Cherry (Solanum lycopersicum var. cerasiforme). Permintaan terhadap tomat cherry di Indonesia sangat tinggi, tetapi hanya dapat dipenuhi sekitar 60% dari produksi dalam negeri, sehingga sisanya harus import. Faktor penyebab kurang maksimalnya produktivitas tanaman tomat cherry antara lain tingkat kesuburan tanah, keadaan cuaca yang tidak menentu, kondisi benih dan adanya serangan hama maupun penyakit. Salah satu cara peningkatan pertumbuhan dan kesehatan tanaman secara preventif dan ramah lingkungan yakni penggunaan rizobakteri. Rizobakteria memiliki peran utama yakni mampu memacu pertumbuhan tanaman melalui fitohormon atau sebagai Biostimulan. Sumber isolat rizobakteria yang sangat melimpah terdapat pada hutan pendidikan UB Forest. Tingginya potensi bakteri Indigenous UB Forest dalam bidang pertanian, sehingga perlu dilakukan penelitian untuk mengetahui kemampuan beberapa isolat tunggal bakteri Indigenous UB Forest sebagai biostimulan tanaman tomat cherry serta pengaruh kandungan senyawa klorofil, karotenoid, dan total fenol terhadap ketahanan dan kesehatan tanaman. Penelitian ini dilaksanakan pada bulan Desember 2021-April 2022. Pelaksanaan penelitian secara in vivo berlangsung di kebun Lily Harvest yang bertempat di Jalan Raya Selekta No. 87, Kelurahan Punten, Kecamatan Bumiaji, Kota Batu, Jawa Timur. Tahap penelitian yang dilakukan meliputi: pembuatan suspensi bakteri Indigenous UB Forest, persiapan bahan tanam, persiapan lahan, penanaman tomat cherry, aplikasi suspensi bakteri sesuai perlakuan, pemeliharaan tanaman, analisa kandungan klorofil dan karotenoid, serta analisa kandungan total fenol menggunakan metode folin-ciocalteu. Pengujian in vivo yang berupa pengaplikasian suspensi bakteri menggunakan Rancangan Acak Kelompok (RAK) dengan 9 perlakuan dan 3 ulangan. Setiap perlakuan terdiri dari 4 tanaman, sehingga total tanaman dalam penelitian ini sebanyak 108 tanaman. Data dianalisis dengan sidik ragam dan diuji lanjut DMRT dengan taraf signifikansi 5% menggunakan Software IBM SPSS ver. 25. Perlakuan aplikasi bakteri Pseudomonas sp. (UB 53), Pseudomonas sp. (UB 52), dan Clostridium sp. (UB 20) memiliki kemampuan dalam memacu pertumbuhan tanaman tomat cherry terhadap tinggi tanaman, jumlah daun, luas daun, bobot basah akar, dan bobot basah tanaman. Sedangkan Aplikasi Bakteri Bacillus sp. (UB 36), Pseudomonas sp. (UB 52), dan Pseudomonas sp. (UB 55) memiliki kemampuan dalam meningkatkan kesehatan tanaman tomat cherry ditinjau dari intensitas penyakit hawar daun, intensitas penyakit layu bakteri, kandungan klorofil total, karotenoid, dan total fenolik. Bakteri Pseudomonas sp. (UB 52) memiliki pengaruh yang baik dalam meningkatkan pertumbuhan dan kesehatan tanaman sehingga berpotensi digunakan sebagai bakteri PGPR (Plant Growth Promoting Rhizobacteria).

English Abstract

The cherry tomato (Solanum lycopersicum var. cerasiforme) is very popular in the world and in high demand. The demand for this type of tomato in Indonesia is very high, but approximately only 60% of demand can be met through domestic production, while the rest must be supplied by importing. There are several factors causing sub-optimal cherry tomato plant productivity, including soil fertility, uncertain weather conditions, seed conditions, pests and diseases. One method to improve plant growth and health in a preventive and environmentally friendly way is by using rhizobacteria. Rhizobacteria have a major role in stimulating plant growth through phytohormones, acting as biostimulants. The most abundant source of rhizobacteria isolates is found in UB Forest. The high potential of Indigenous UB Forest bacteria in agriculture makes it necessary to conduct research in determining the potential of several single isolates of Indigenous UB Forest bacteria as biostimulants for cherry tomato plant and the effect of chlorophyll, carotenoid, and total phenol compounds on plant resistance and health. This research was conducted in December 2021 to April 2022. The research took place in vivo in the Lily Harvest garden located at Jalan Raya Selekta No. 87, Punten Village, Bumiaji District, Batu City, of East Java. The research consisted of several stages, including making suspension of Indigenous UB Forest bacteria, preparing planting medium, preparing planting space, planting cherry tomato, applying bacterial suspension according to treatment, performing plant maintenance, analysing chlorophyll and carotenoid content, and analysing total phenol content using the Folin-Ciocalteu method. The in vivo testing in the form of application of bacterial suspension using a Randomized Block Design was done with 9 treatments and 3 replications. Each treatment consisted of 4 plants, with the total number of plants used in this research amounted to 108 plants. The obtained data were then analysed by means of variance and further tested by DMRT with a significance level of 5% using the IBM SPSS software ver. 25. The application treatments of Pseudomonas sp. (UB 53), Pseudomonas sp. (UB 52), and Clostridium sp. (UB 20) have been shown to stimulate the growth of cherry tomato plant on the aspects of plant height, number of leaves, leaf area, root wet weight, and plant wet weight. Meanwhile, the application treatments of Bacillus sp. (UB 36), Pseudomonas sp. (UB 52), and Pseudomonas sp. (UB 55) have been shown to improve the health of cherry tomato plant in terms of the intensity of leaf blight, the intensity of bacterial wilt disease, the total chlorophyll content, carotenoids, and phenolics. Pseudomonas sp. (UB 52) has a good effect in increasing plant growth and health, offering the potential to be used as PGPR (Plant Growth Promoting Rhizobacteria)

Other obstract

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Item Type: Thesis (Sarjana)
Identification Number: 0522040264
Subjects: 600 Technology (Applied sciences) > 632 Plant injuries, diseases, pests > 632.6 Animal pests
Divisions: Fakultas Pertanian > Hama dan Penyakit Tanaman
Depositing User: Nur Cholis
Date Deposited: 03 Jan 2023 03:44
Last Modified: 03 Jan 2023 03:44
URI: http://repository.ub.ac.id/id/eprint/196541
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