Studies on the induction of oxidative stress by 4-tert-octylphenol in Ceratophyllum demersum.

Cahyanurani, Annisa’ Bias and Dr. Tsung-Meng Wu, - and Dr. Chun-Hung Liu, - and Dr. Uun Yanuhar, S.Pi., M.Si. (2016) Studies on the induction of oxidative stress by 4-tert-octylphenol in Ceratophyllum demersum. Magister thesis, Universitas Brawijaya.



English Abstract

Alkylphenol ethoxylates (APEOs) are surfactants which have been widely used in a variety of commercial products and can be degraded in the aquatic environment becoming more toxic metabolites. 4-tert-octylphenol (OP) is one of the primary breakdown products of APEOs with endocrine disrupting properties that has been known as persistent and ubiquitos pollutant. In order to investigate the effect of OP toxicity to aquatic plant, the submersed macrophyte Ceratophyllum demersum was chosen to treat with various concentrations of OP (0, 0.5, 1, 1.5, 2 and 3 mg L-1) for 5 days. The toxic effect and oxidative stress caused by OP resulted in an inhibition of growth rate, reduction of total chlorophyll content (chlorophyll a and b) and an increase in the levels of reactive oxygen species (ROS), O2•− and H2O2. However, there was no significant change in the content of malondialdehyde (MDA). The antioxidative enzyme activities showed a significant increase in superoxide dismutase (SOD), guaiacol peroxidase (POD), catalase (CAT), gluthathione reductase (GR) and ascorbate peroxidase (APX). The contents of non-enzymatic antioxidants, ascorbate (AsA) and glutathione (GSH), were also significantly increased under OP exposure. To confirm the role of GSH in C. demersum under OP exposure, BSO, a specific and potent inhibitor of GSH biosynthesis, was used. After BSO pretreatment, the total GSH content was significantly reduced. The decreasing of total GSH indicated that the synthesis of GSH has been blocked, it was followed by the decreasing of total AsA content and also GR and APX enzyme activity. Interestingly, C. demersum showed much more severe phenotype under OP exposure with BSO pretreatment. In conclusion, C. demersum might actively regulate the antioxidant machinery, especially GSH biosynthesis, to against OP-induced oxidative stress.

Item Type: Thesis (Magister)
Identification Number: TES/583.29/CAH/s/041608000...
Uncontrolled Keywords: 4-tert-octylphenol, Ceratophyllum demersum, oxidative stress, ROS, GSH.
Subjects: 500 Natural sciences and mathematics > 583 Magnoliopsida (Dicotyledons) > 583.2 Magnoliidae
Divisions: S2/S3 > Magister Budidaya Perairan, Fakultas Perikanan dan Ilmu Kelautan
Depositing User: Zainul Mustofa
Date Deposited: 21 Oct 2022 03:37
Last Modified: 21 Oct 2022 03:37
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